Induction of micronuclei and erythrocyte alterations in the catfish Clarias batrachus by 2,4-dichlorophenoxyacetic acid and butachlor

Ateeq, Bushra; Farah, Mohammad Abul; Ali, M. Niamat; Ahmad, Waseem

doi:10.1016/s1383-5718(02)00075-x

Cited by 145 publications

(74 citation statements)

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“…There are a number of experimental studies which demonstrates, that nuclear abnormalities other than MN are sensitive and informative biomarkers (Pacheco and Santos, 1999;Gracia-Vazquez, 2000, 2001;Ateeq et al, 2002;Çavaş and Ergene-Gözükara, 2003;Baršienė et al, 2006a;Baršienė and Andreikėnaitė, 2007). Mutagenic potential of secondary-treated bleached pulp mill effluents was revealed by laboratory and field experiments performed by Pacheco and Santos (1999).…”

Section: Discussionmentioning

confidence: 99%

“…Exposure of Nile tilapia (Oreochromis niloticus) with different concentrations of textile mill effluent and cyclophosphamide induced dose-dependent increase of MN as well as binuclei, lobed, blebbed and notched nuclei in peripheral blood erythrocytes (Çavaş and Ergene-Gözükara, 2003). Two herbicides, 2,4-dichlorophenoxyacetic acid and butachlor induced dose and time dependant genotoxic and cytotoxic effects in peripheral erythrocytes of catfish (Clarias batrachus) (Ateeq et al, 2002). In eel treatment with 0.3, 0.9 and 2.7 µM of naphtalene caused induction of MN and other nuclear abnormalities (Teles et al, 2003).Elevated frequencies of erythrocytes with MN, lobed and blebbed nuclei were observed in butterfish (Pholis gunnellus) inhabiting contaminated areas of the Firth of Forth, Scotland (Bombail et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, growing attention has been paid to nuclear abnormalities other than MN and it was demonstrated that these nuclear abnormalities can serve as an index of genotoxic and cytotoxic damage (Carrasco et al, 1990;Dolcetti and Venier, 2002;Ateeq et al, 2002;Pacheco and Santos, 2002;Santos, 2002, 2003;Ergene-Gözükara, 2003, 2005b;Frenzilli et al, 2004, Bolognesi et al, 2006b, Baršienė et al, 2006a, 2006cBaršienė and Andreikėnaitė, 2007). Nuclear buds are morphologically similar to micronuclei with the exception that they are joined to the nucleus by a thin nucleoplasmic connection.…”

Section: Introductionmentioning

confidence: 99%

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Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Rybakovas

Baršienė

Lang

2009

Marine Environmental Research

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Please cite this article as: Rybakovas, A., Baršienė, J., Lang, T., Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and North Seas, Marine Environmental Research (2009Research ( ), doi: 10.1016Research ( /j.marenvres. 2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT AbstractMicronuclei (MN), nuclear buds (NB) and fragmented-apoptotic cells (FA) were analyzed in mature peripheral blood and immature cephalic kidney erythrocytes of flounder (Platichthys flesus), dab (Limanda limanda) and cod (Gadus morhua) from 12 offshore sites in the Baltic Sea (479 specimens) and 11 sites in the North Sea (291 specimens), which were collected during three research vessel cruises in December 2002 and in September 2004. The highest levels of environmental genotoxicity (frequencies of MN up to 0.5 ‰, NB -up to 0.75 ‰) and cytotoxicity (FA -up to 0.53 ‰) were observed in flatfishes from areas close to oil and gas platforms in the North Sea and in zones related to the extensive shipping and potentially influenced by contamination from large European Rivers (Elbe, Vistula, Oder). In dab from the offshore zones of the North Sea, the levels of nuclear abnormalities were higher as compared to those in dab from the Baltic Sea. Responses in immature kidney erythrocytes were higher than in mature erythrocytes from peripheral blood. MN frequency lower than 0.05 ‰ (the Baltic Sea) and lower than 0.1 ‰ (the North Sea) could be suspected as a reference level in the peripheral blood erythrocytes of flatfish.Keywords: Genotoxicity; Cytotoxicity; Micronuclei; Nuclear abnormalities; Erythrocytes; Baltic Sea; North Sea. ACCEPTED MANUSCRIPT 2 IntroductionSurrounded by European countries, the Baltic and the North Seas are constantly exposed to anthropogenic pressure. With pollution from land-based discharges, coastal industries, municipal outflows, agricultural activities, oil and gas industry (drilling and transportation) and transport activities, thousands of chemicals are entering the marine environment. A large proportion of these compounds are potentially toxic, genotoxic and carcinogenic to aquatic organisms (Jha, 2004(Jha, , 2008. The interaction of genotoxic contaminants with DNA causes various genetic disturbances, which often are irreversible and can be transmitted to the next generations (Depledge and Fossi, 1994;Dixon et al., 1999;Jha, 2004). The analysis of environmental genotoxicity provides early warning signs of adverse long-term effects of contamination. A number of biomarkers have been applied for assessment of environmental genotoxicity and the micronuclei (MN) test,...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Rybakovas

Baršienė

Lang

2009

Marine Environmental Research

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“…The micronucleus test is often used to predict the carcinogenic potential of compounds (Sato et al, 2001), although the scope of this test is continuously broadening by incorporating new technologies for the detection of different genetic alterations (Zuñiga González et al, 2001a, b;Ateeq et al, 2002;Cristaldi et al, 2004). Several authors have reported modifications to the test, including alterations in culture media, β-cytochalasin concentration, osmolarity, pH and staining techniques, all of which are capable of introducing artifacts and producing intra-and inter-laboratory variability (Fenech et al, 2003;Mentières and Marzin, 2004).…”

mentioning

confidence: 99%

The bone marrow micronucleus test and metronidazole genotoxicity in different strains of mice (Mus musculus)

Abrevaya¹,

Carballo²,

Mudry³

2007

Genet. Mol. Biol.

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The mouse (Mus musculus) bone marrow micronucleus test was carried out using 24 outbred National Institutes of Health (NIH) mice, 24 inbred Swiss Webster (CFW) mice and 20 inbred Bagg albino/color locus Jackson (BALB/cJ) mice. The mice in the experimental group (n = 32) were injected intraperitoneally with 133 mg kg -1 of metronidazole parenteral solution and the control group consisted of mice (n = 36) which had not been injected with metronidazole. There was no significant difference (p > 0.05) between the sexes regarding the micronucleus frequency in either the experimental or the control group. When the Mn frequencies of the three strains were compared, the results for the CFW and BALB/cJ strains did not differ statistically (p > 0.05) for either the experimental or control groups but there were significant (p < 0.05) differences between the CFW and NIH strains and the NIH and BALB/cJ strains for the experimental and control groups, with the NIH strain always showing the highest micronucleus frequency. Our results also show that metronidazole was possible genotoxic agent because it produced a significant increase (p < 0.05) in the micronucleus frequency of the experimental group as compared to the control group for all the three mouse strains tested.

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“…A genética toxicológica surgiu da preocupação com a vulnerabilidade do material genético às agressões impostas pela contaminação ambiental (RABELLO -GAY et al, 1991 Entre os vegetais superiores utilizados como organismos-teste, a espécie de Allium cepa vem se destacando devido a sua eficiência na detecção de danos genéticos a partir da avaliação de aberrações cromossômicas e micronúcleos induzidos por poluentes ambientais (FISKESJÖ, 1985;RAN;NIELSEN, 1994;SMAKA-KINCL et al, 1996;MATSUMOTO et al, 2006;FATIMA;AHMAD, 2006;MIGID et al, 2007), bem como por sustâncias químicas puras (FISKESJÖ, 1982(FISKESJÖ, , 1985(FISKESJÖ, , 1988RANK et al, 1993;NIELSEN, 1997;ATEEQ et al, 2002;TÜRKOGLU, 2007). Além da sensibilidade, a espécie de A. cepa tem sido indicada por vantagens como baixo custo, fácil manuseio e por apresentar cromossomos grandes e em número reduzido (2n=16), sendo ideal nos estudos de avaliação de danos cromossômicos e/ou de distúrbios do ciclo de divisão celular (FISKESJÖ, 1985, MATSUMOTO et al, 2006FERNANDES et al, 2007).…”

Section: Genotoxicidadeunclassified

Ecotoxicologia de um sistema florestal de eucalipto tratado com lodo de esgoto

Brossi¹

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Induction of micronuclei and erythrocyte alterations in the catfish Clarias batrachus by 2,4-dichlorophenoxyacetic acid and butachlor

Cited by 145 publications

References 29 publications

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

Environmental genotoxicity and cytotoxicity in the offshore zones of the Baltic and the North Seas

The bone marrow micronucleus test and metronidazole genotoxicity in different strains of mice (Mus musculus)

Ecotoxicologia de um sistema florestal de eucalipto tratado com lodo de esgoto

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