Induction of Melanin Synthesis in Albino Mouse Skin by Dna From Pigmented Mice

Ottolenghi-Nightingale, Elena

doi:10.1073/pnas.64.1.184

Cited by 20 publications

(1 citation statement)

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“…Whether this can be attributed to inadequacies in technique or cloning, a special requirement for competence in the recipient cell population, or a variability in the biological activity of the isolated DNA is not known at this time. Despite improvements in understanding and technique, mammalian celltransformation studies have often given variable results (6,18,19). These problems might be minimized if the efficiency of DNA-mediated change and selection of altered cells could be improved.…”

Section: Interaction Of Chinese Hamster Cells and Dna From Ehrlich Asmentioning

confidence: 99%

Studies of Dna-Induced Heritable Alteration of Mammalian Cells

Borenfreund

Honda

Steinglass

et al. 1970

The Journal of Experimental Medicine

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The chemical nature of genetic determinants was eludicated in 1944 by Avery, MacLeod, and McCarty in their classic finding of DNA-mediated transformation of pneumococcal types (1). This provided knowledge of the kinds of biochemical phenomena which are directly controlled by DNA, in bacteria. That DNA is involved in similar functions in somatic mammalian cells is not in dispute; rigorous experimental evidence however has been difficult to obtain (2). Heritable acquisition of genetic activity has resulted from the "infection" of mammalian cells by DNA isolated from oncogenic viruses (3-5), but convincing examples of transformation by mammalian DNA have beenvery rare indeed. Perhaps the most persuasive instance of such change is the recent report (6) that the implantation of amelanotic cells, after incubation with DNA isolated from melanin-producing embryonic cells, into white mice, induced the formation of melanin-producing nodules; unfortunately, controls with DNA from white mice or other sources were not included.The present studies are concerned with heritable acquisition of cellular genetic properties by the exposure of cells to genetically distinct cells or to D N A isolated from them. The properties chosen were the ability to synthesize mouse gene products (antigens), and the expression of oncogenic potential. The donors were mouse Ehrlich ascites tumor cells and the recipients were nonmalignant Chinese hamster cells. A preliminary account of the background and the results of our earlier experiments has appeared (7). Materials and MethodsCells and Reagents.---Chinese hamster (CH) 1 cells were used preferentially because their easily recognizable chromosomes (11 = 22) greatly simplify study of their karyotypes (8, 9).

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Section: Interaction Of Chinese Hamster Cells and Dna From Ehrlich Asmentioning

confidence: 99%