Induction of M2 Polarization in Primary Culture Liver Macrophages from Rats with Acute Pancreatitis

Xu, Lixia; Yang, Fang; Lin, Rong; Han, Chaoqun; Li, Jun

doi:10.1371/journal.pone.0108014

Cited by 21 publications

(17 citation statements)

References 28 publications

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“…More importantly, the levels of plasma CRP were positively correlated with the numbers of CD14+CD163- and CD14+CD163-MAC387+ M1 monocytes in the MAP patients. These data were consistent with a previous report of M1-polarized macrophages at the early stage in rats with AP[ 29 ]. Similarly, our previous study had found that peripheral blood polarized M1 monocytes are present in patients with tuberculous pleural effusion (TPE)[ 15 ].…”

Section: Discussionsupporting

confidence: 94%

Different phenotypes of monocytes in patients with new-onset mild acute pancreatitis

Zhang

Jiang

Wang

et al. 2017

WJG

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AIMTo evaluate the numbers of different subsets of monocytes and their associations with the values of clinical measures in mild acute pancreatitis (MAP) patients.METHODSThe study included one group of 13 healthy controls and another group of 24 patients with new-onset MAP. The numbers of different subsets of monocytes were examined in these two groups of subjects by flow cytometry. The concentrations of plasma interleukin (IL)-10 and IL-12 were determined by cytometric bead array. The acute physiology and chronic health evaluation (APACHE) II scores of individual patients were evaluated, and the levels of plasma C-reactive protein (CRP) as well as the activities of amylase and lipase were measured.RESULTSIn comparison with that in the controls, significantly increased numbers of CD14+CD163-, CD14+CD163-MAC387+ M1 monocytes, but significantly reduced numbers of CD14+CD163+IL-10+ M2 monocytes were detected in the MAP patients (P < 0.01 or P < 0.05). Furthermore, significantly higher levels of plasma IL-10 and IL-12 were observed in the MAP patients (P < 0.01 for all). More importantly, the levels of plasma CRP were positively correlated with the numbers of CD14+CD163- (R = 0.5009, P = 0.0127) and CD14+CD163-MAC387+ (R = 0.5079, P = 0.0113) M1 monocytes and CD14+CD163+CD115+ M2 monocytes (R = 0.4565, P = 0.0249) in the patients. The APACHE II scores correlated with the numbers of CD14+CD163+CD115+ (R = 0.4581, P = 0.0244) monocytes and the levels of plasma IL-10 (R = 0.4178, P = 0.0422) in the MAP patients. However, there was no significant association among other measures tested in this population.CONCLUSIONIncreased numbers of CD14+CD163- and CD14+ CD163-MAC387+ monocytes may contribute to the pathogenesis of MAP, and increased numbers of CD14+CD163+CD115+ monocytes may be a biomarker for evaluating the severity of MAP.

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Section: Discussionsupporting

confidence: 94%

Different phenotypes of monocytes in patients with new-onset mild acute pancreatitis

Zhang

Jiang

Wang

et al. 2017

WJG

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“…The M1 state was confirmed by the up-regulated expression of M1 phenotypes and down-regulated expression of M2 phenotypes. And the results conform to the conventional theory that macrophages can induce pro-inflammatory cytokines and amplify the degree of the inflammation during early phase of SAP[31,32]. …”

Section: Discussionsupporting

confidence: 85%

IRF5 regulates lung macrophages M2 polarization during severe acute pancreatitisin vitro

Sun¹,

He²,

Qu³

et al. 2016

WJG

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AIMTo investigate the role of interferon regulatory factor 5 (IRF5) in reversing polarization of lung macrophages during severe acute pancreatitis (SAP) in vitro.METHODSA mouse SAP model was established by intraperitoneal (ip) injections of 20 μg/kg body weight caerulein. Pathological changes in the lung were observed by hematoxylin and eosin staining. Lung macrophages were isolated from bronchoalveolar lavage fluid. The quantity and purity of lung macrophages were detected by fluorescence-activated cell sorting and evaluated by real-time polymerase chain reaction (RT-PCR). They were treated with IL-4/IRF5 specific siRNA (IRF5 siRNA) to reverse their polarization and were evaluated by detecting markers expression of M1/M2 using RT-PCR.RESULTSSAP associated acute lung injury (ALI) was induced successfully by ip injections of caerulein, which was confirmed by histopathology. Lung macrophages expressed high levels of IRF5 as M1 phenotype during the early acute pancreatitis stages. Reduction of IRF5 expression by IRF5 siRNA reversed the action of macrophages from M1 to M2 phenotype in vitro. The expressions of M1 markers, including IRF5 (S + IRF5 siRNA vs S + PBS, 0.013 ± 0.01 vs 0.054 ± 0.047, P < 0.01), TNF-α (S + IRF5 siRNA vs S + PBS, 0.0003 ± 0.0002 vs 0.019 ± 0.018, P < 0.001), iNOS (S + IRF5 siRNA vs S + PBS, 0.0003 ± 0.0002 vs 0.026 ± 0.018, P < 0.001) and IL-12 (S + IRF5 siRNA vs S + PBS, 0.000005 ± 0.00004 vs 0.024 ± 0.016, P < 0.001), were decreased. In contrast, the expressions of M2 markers, including IL-10 (S + IRF5 siRNA vs S + PBS, 0.060 ± 0.055 vs 0.0230 ± 0.018, P < 0.01) and Arg-1 (S + IRF5 siRNA vs S + PBS, 0.910 ± 0.788 vs 0.0036 ± 0.0025, P < 0.001), were increased. IRF5 siRNA could reverse the lung macrophage polarization more effectively than IL-4.CONCLUSIONTreatment with IRF5 siRNA can reverse the pancreatitis-induced activation of lung macrophages from M1 phenotype to M2 phenotype in SAP associated with ALI.

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“…Thus, in models of alcoholic and nonalcoholic liver disease, M2-polarized Kupffer cells induced apoptosis of Kupffer cells with an M1-phenotype and that had positive effect on the state of the liver [133]. Similar data were obtained on a model of acute pancreatitis in rats [134]. In pancreatitis, the accumulation of Kupffer cells with the M1 phenotype in the liver is observed.…”

Section: Potential Therapeutic Approachesmentioning

confidence: 75%

Phenotypical and Functional Polymorphism of Liver Resident Macrophages

Elchaninov

Fatkhudinov

Vishnyakova

et al. 2019

Cells

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Liver diseases are one of the main causes of mortality. In this regard, the development of new ways of reparative processes stimulation is relevant. Macrophages play a leading role in the regulation of liver homeostasis in physiological conditions and in pathology. In this regard, the development of new liver treatment methods is impossible without taking into account this cell population. Resident macrophages of the liver, Kupffer cells, represent a unique cell population, first of all, due to their development. Most of the liver macrophages belong to the self-sustaining macrophage cell population, whose origin is not bone marrow. In addition, Kupffer cells are involved in such processes as regulation of hepatocyte proliferation and apoptosis, remodeling of the intercellular matrix, lipid metabolism, protective function, etc. Such a broad spectrum of liver macrophage functions indicates their high functional plasticity. The review summarizes recent data on the development, phenotypic and functional plasticity, and participation in the reparative processes of liver macrophages: resident macrophages (Kupffer cells) and bone marrow-derived macrophages.

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Induction of M2 Polarization in Primary Culture Liver Macrophages from Rats with Acute Pancreatitis

Cited by 21 publications

References 28 publications

Different phenotypes of monocytes in patients with new-onset mild acute pancreatitis

Different phenotypes of monocytes in patients with new-onset mild acute pancreatitis

IRF5 regulates lung macrophages M2 polarization during severe acute pancreatitisin vitro

Phenotypical and Functional Polymorphism of Liver Resident Macrophages

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