Induction of lipid metabolism dysfunction, oxidative stress and inflammation response by tris(1-chloro-2-propyl)phosphate in larval/adult zebrafish

Yan, Jin; Zhao, Zelin; Xia, Min; Chen, Shuya; Wan, Xiancheng; He, Anfei; Sheng, Guangyao; Wang, Xuedong; Qian, Qiuhui; Wang, Huili

doi:10.1016/j.envint.2022.107081

Cited by 24 publications

(10 citation statements)

References 74 publications

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“…Changes in the mRNA transcription levels of certain genes related to lipid metabolism induced by the OPEs were also observed in several previous studies. Specially, Yan et al reported that tris(1-chloro-2-propyl)phosphate (TCIPP) exposure increased the expression level of fasn but decreased the expression level of ppar α and cpt1 in zebrafish, which agreed with the present results . Moreover, treatment with EHDPP also significantly changed the transcription of genes associated with lipid metabolism in zebrafish, including up-regulation of cpt1 , ldlr , ppar α, and fasn and down-regulation of ppar γ and cyp7a1 .…”

Section: Resultssupporting

confidence: 91%

“…However, the silver carp with different developmental stages should be used to further confirm the lipid metabolic disorders induced by OPEs in future studies. Similar to our findings, exposure to great concentrations of tris(1-chloro-2-propyl)phosphate (TCIPP) led to significant increases in the contents of TG and CHO in both larval and adult zebrafish . Elevated CHO and TG levels were reported to be associated with a serious cardiovascular disease, suggesting that the lipid accumulation induced by OPE exposure probably poses health threat to silver carp.…”

Section: Resultssupporting

confidence: 85%

“…Specially, Yan et al reported that tris(1chloro-2-propyl)phosphate (TCIPP) exposure increased the expression level of fasn but decreased the expression level of pparα and cpt1 in zebrafish, which agreed with the present results. 27 Moreover, treatment with EHDPP also significantly changed the transcription of genes associated with lipid metabolism in zebrafish, including up-regulation of cpt1, ldlr, pparα, and fasn and down-regulation of pparγ and cyp7a1. 57 Though some gene expressions were not consistent with our results, the above results both demonstrated that OPEs could lead to significant changes in the transcription of genes involved in lipid metabolism.…”

Section: Opes-induced Changes In Hepatic Lipidmentioning

confidence: 95%

“…Similar to our findings, exposure to great concentrations of tris(1-chloro-2-propyl)phosphate (TCIPP) led to significant increases in the contents of TG and CHO in both larval and adult zebrafish. 27 Elevated CHO and TG levels were reported to be associated with a serious cardiovascular disease, 28 suggesting that the lipid accumulation induced by OPE exposure probably poses health threat to silver carp. Actually, it was verified that exposure to mixed OPEs was positively related to the increased prevalence of cardiovascular disease among US adults.…”

Section: Ope-induced Lipid Metabolism Disorder In Laboratory Exposurementioning

confidence: 99%

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Lipid Metabolic Disorders Induced by Organophosphate Esters in Silver Carp from the Middle Reaches of the Yangtze River

Liu,

Zhang,

et al. 2024

Environ. Sci. Technol.

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The Yangtze River fishery resources have declined strongly over the past few decades. One suspected reason for the decline in fishery productivity, including silver carp (Hypophthalmichthys molitrix), has been linked to organophosphate esters (OPEs) contaminant exposure. In this study, the adverse effect of OPEs on lipid metabolism in silver carp captured from the Yangtze River was examined, and our results indicated that muscle concentrations of the OPEs were positively associated with serum cholesterol and total lipid levels. In vivo laboratory results revealed that exposure to environmental concentrations of OPEs significantly increased the concentrations of triglyceride, cholesterol, and total lipid levels. Lipidome analysis further confirmed the lipid metabolism dysfunction induced by OPEs, and glycerophospholipids and sphingolipids were the most affected lipids. Hepatic transcriptomic analysis found that OPEs caused significant alterations in the transcription of genes involved in lipid metabolism. Pathways associated with lipid homeostasis, including the peroxisome proliferator-activated receptor (PPAR) signal pathway, cholesterol metabolism, fatty acid biosynthesis, and steroid biosynthesis, were significantly changed. Furthermore, the affinities of OPEs were different, but the 11 OPEs tested could bind with PPARγ, suggesting that OPEs could disrupt lipid metabolism by interacting with PPARγ. Overall, this study highlighted the harmful effects of OPEs on wild fish and provided mechanistic insights into OPE-induced metabolic disorders.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 85%

Section: Opes-induced Changes In Hepatic Lipidmentioning

confidence: 95%

Section: Ope-induced Lipid Metabolism Disorder In Laboratory Exposurementioning

confidence: 99%

See 2 more Smart Citations

Lipid Metabolic Disorders Induced by Organophosphate Esters in Silver Carp from the Middle Reaches of the Yangtze River

Liu,

Zhang,

et al. 2024

Environ. Sci. Technol.

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“…S2B, Supporting Information. Several chemicals have previously been found to act as obesogens using this assay, including TBBPA and TCBPA [24] , tris(1-chloro-2-propyl)phosphate (TCPP) [47] ], perfluorooctanesulfonic acid (PFOS) [48] , or a mixture of endocrine disruptors [49].…”

Section: Acetochlor Exposure Induces Lipid Accumulation In Zebrafish ...mentioning

confidence: 99%

The herbicide acetochlor causes lipid peroxidation by inhibition of glutathione peroxidase 4

Mesmar

Muhsen

Tourigny

et al. 2023

Preprint

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Obesity is increasing worldwide, particularly in rural communities, where people are likely exposed to high levels of pesticides. We here investigated whether six commonly used agricultural pesticides on corn and soy fields have adipogenic activity and act as obesogens. Exposure to two pesticides, the herbicides acetochlor and metolachlor, induced adipogenesisin vitroin mouse 3T3-L1 preadipocytes. The most potent compound, acetochlor, was selected for further studies in zebrafish. Acetochlor exposure caused morphological malformations and lethality in zebrafish larvae with an EC50of 7.8 µM and an LC50of 12 µM. Acetochlor exposure also resulted in lipid accumulation is zebrafish larvae when simultaneously fed a high cholesterol diet. To decipher the molecular mechanisms behind acetochlor action, we preformed transcriptomic and targeted lipidomic analysis of exposed animals. The combined omics results suggested that acetochlor exposure increased Nrf2 activity in response to reactive oxygen species, as well as induced lipid peroxidation and ferroptosis. We further discovered that acetochlor structurally shares a chloroacetamide group with known inhibitors of glutathione peroxidase 4 (GPX4). Computational docking analysis suggested that acetochlor covalently binds to the active site of GPX4. Consequently, Gpx4 activity was efficiently repressed by acetochlor, and lipid peroxidation was increased in zebrafish. We propose that acetochlor disrupts lipid homeostasis by inhibiting Gpx4, resulting in accumulation of lipid peroxidation, 4-hydroxynonenal, and reactive oxygen species in the cells, which in turn activate Nrf2. Because metolachlor, among other acetanilide herbicides, also contain the chloroacetamide group, inhibition of Gpx4 activity may represent a novel, common molecular initiating event of obesogens.SynopsisRural populations have a high prevalence of metabolic disease and are highly exposed to pesticides. This study reports that the herbicide acetochlor, heavily used on soy and corn fields, inhibits an enzyme that protects from oxidation of lipids in the cell membrane, oxidative stress and a type of cell death called ferroptosis, features that are linked to metabolic disruption and obesity.

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Association between organophosphate esters individual and mixed exposure with the risk of hyperlipidemia and serum lipid levels among adults in Wuhan, China

Xu,

Xie,

Yang

et al. 2024

Environ Sci Pollut Res

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Induction of lipid metabolism dysfunction, oxidative stress and inflammation response by tris(1-chloro-2-propyl)phosphate in larval/adult zebrafish

Cited by 24 publications

References 74 publications

Lipid Metabolic Disorders Induced by Organophosphate Esters in Silver Carp from the Middle Reaches of the Yangtze River

Lipid Metabolic Disorders Induced by Organophosphate Esters in Silver Carp from the Middle Reaches of the Yangtze River

The herbicide acetochlor causes lipid peroxidation by inhibition of glutathione peroxidase 4

Association between organophosphate esters individual and mixed exposure with the risk of hyperlipidemia and serum lipid levels among adults in Wuhan, China

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