Induction of  -Lactamase in Proteus vulgaris

Okonogi, Kenji; Kuno, Mitsuzo; Higashide, Eiji

doi:10.1099/00221287-132-1-143

Cited by 15 publications

(9 citation statements)

References 12 publications

(15 reference statements)

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“…bacteria are ,-lactamase positive or ,-lactamase negative (1,6). Moreover, it is well known that the ,-lactamase inducibility of bacteria depends markedly on the P-lactam antibiotic used and its concentration in vitro (8,10). In this study, we have shown that this phenomenon could also take place in vivo.…”

Section: Discussionsupporting

confidence: 51%

Paradoxical activity of beta-lactam antibiotics against Proteus vulgaris in experimental infection in mice

Ikeda

Fukuoka

Motomura

et al. 1990

Antimicrob Agents Chemother

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reported that many of the 7-aminothiazolyl cephalosporins, such as cefmenoxime, showed paradoxically reduced activity against Proteus vulgaris at higher concentrations, whereas these paradoxical effects were not observed for other types of cephalosporins, such as cefbuperazone and cefoperazone. In this study, we compare the therapeutic effect of cefmenoxime with that of cefbuperazone and explore the in vivo paradoxical effect of cefmenoxime by using an experimental infection model in mice. In an intraperitoneal infection with P. vulgaris 11, the survival rate with cefmenoxime was increased to 43% at 3.13 mg/kg but was lower at higher doses. On the other hand, cefbuperazone did not show such a paradoxical therapeutic effect. In mice infected with P. vulgaris 11, cefmenoxime levels in both serum and peritoneal washings were rapidly reduced and f-lactamase activities in the peritoneal cavity were increased at higher cefmenoxime doses. These findings suggested that high levels of cefmenoxime at the infection site induced increased production of j(-lactamase, which then rapidly inactivated the antibiotic. We conclude that the paradoxical therapeutic effect of cefmenoxime against P. vulgaris occurs by the same mechanisms as the in vitro effect and that the high P-lactamase inducibility and low (-lactamase stability may account for the paradoxical therapeutic effect of cefmenoxime against P. vulgaris.The antibacterial activity of an antibiotic is generally proportional to its concentration. However, several exceptions to this trend have been reported (3, 9, 13). In previous papers (4, 5), we reported that some 7-aminothiazolyl-type cephalosporins showed paradoxically reduced activities against Proteus vulgaris when they were present at high concentrations. Indeed, it has been difficult to demonstrate such paradoxical antibacterial activity clinically, but it appears that the in vivo paradoxical effect can be confirmed by using an experimental infection model. Eagle et al. (2), using an animal model of Staphylococcus aureus infection, found that the paradoxical antibacterial effect holds true in vivo. However, the mechanism of this effect was not explained sufficiently. In this study, we confirm that cefmenoxime has a paradoxical therapeutic effect in an experimental intraperitoneal model of P. vulgaris infection in mice. We also investigate the mechanism involved. MATERIALS AND METHODSAntibiotics. Cefmenoxime, prepared by Takeda Chemical Industries, Co., Ltd., Osaka, Japan, and cefbuperazone, prepared by Toyama Chemical, Co., Ltd., Tokyo, Japan, were used as the test antibiotics.Bacterial strains. P. vulgaris 11 and P. vulgaris 11-S, a ,-lactamase-noninducing mutant of P. vulgaris 11 (4), were used in this study.Intraperitoneal infection. Male ICR strain mice (Shizuoka Agricultural Cooperative Associations for Laboratory Animals, Shizuoka, Japan) weighing 18 to 19 g were used in this study. Bacterial cells from an overnight culture on heart infusion agar (Eiken Chemical, Tokyo, Japan) were suspended in physiological...

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Section: Discussionsupporting

confidence: 51%

Paradoxical activity of beta-lactam antibiotics against Proteus vulgaris in experimental infection in mice

Ikeda

Fukuoka

Motomura

et al. 1990

Antimicrob Agents Chemother

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“…The result supports the findings of (Nordstrom & Sykes, 1973;Okonogi, Kuno,& Higashide, 1986;Raimondi, Sisto & Nikaido, 2007) who reported that the specific activity of β-lactamase increased linearly with increasing concentrations of inducer. In addition, Khan, Sallum, Zheng, Nau & Hassan (2014) in their study on rapid determination of β-lactamase and antibiotic activity detected β-lactamase in the absence of inducer however, at a lower level when compared with induction.…”

Section: Discussionsupporting

confidence: 81%

Beta-lactamase Induction in Nosocomial Staphylococcus aureus Strains Cultured from the Nasal Cavity and Wound of Patients

Torimiro¹,

T²

2017

IJB

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The study was conducted to determine β-lactamase induction in Staphylococcus aureus strains cultured from the nasal cavity and surgical wounds of in-patients at a tertiary teaching hospital.Swab samples were taken from the nasal cavity -wound pair of 110 consented patients. The S. aureus isolates were identified and the antibiotics susceptibility tests were conducted using standard protocols. β-lactamase induction was assayed with the micro-iodometric method. The data obtained were statistically analysed.A total of 81 S. aureus isolates were cultured from the nasal cavity and wounds of the 110 samples collected. β-lactamase was produced by 14 (41.2%) and 26 (55.3%) of the isolates cultured from the nasal cavity and wounds respectively. Constitutive and induced β -lactamase production was observed.β-lactamase production in the isolates is not dependent on antibiotic exposure alone. The findings underscore the need for appropriate infection control and adequate hygiene practices to prevent the spread of S. aureus in the hospital settings.

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“…Other Proteus vulgaris cephalosporinases have similar substrate profiles but slightly different molecular sizes and isoelectric points: strain TN1945, pI 8.8; molecular mass, 28 kDa; strain GN4413, pI 8.2; molecular mass, 27.5 kDa; strain GN4818, pI 6.9; molecular mass, 27 kDa (212).…”

Section: Discussionmentioning

confidence: 99%

A functional classification scheme for beta-lactamases and its correlation with molecular structure

Bush¹,

Jacoby²,

Medeiros³

1995

Antimicrob Agents Chemother

2,211

1,680

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Induction of -Lactamase in Proteus vulgaris

Cited by 15 publications

References 12 publications

Paradoxical activity of beta-lactam antibiotics against Proteus vulgaris in experimental infection in mice

Paradoxical activity of beta-lactam antibiotics against Proteus vulgaris in experimental infection in mice

Beta-lactamase Induction in Nosocomial Staphylococcus aureus Strains Cultured from the Nasal Cavity and Wound of Patients

A functional classification scheme for beta-lactamases and its correlation with molecular structure

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