Induction of intestinal ischemia reperfusion injury by portal vein outflow occlusion in rats

Vincenti, Marco; Behrends, Matthias; Dang, Kim; Park, Yeon H.; Hirose, Ryutaro; Blasi-Ibanez, Annabel; Liu, Tao; Serkova, Natalie J.; Niemann, Claus U.

doi:10.1007/s00535-010-0262-0

Cited by 20 publications

(17 citation statements)

References 20 publications

(25 reference statements)

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“…Moreover, greater degree of intestinal mucosa damage in isolated venous occlusion has been seen versus arterial occlusion. Similar insults, local or remote, result from the occlusion of both mesenteric vessels 11,12 .…”

Section: Discussionmentioning

confidence: 99%

Pentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats

Marques

Rasslan²,

Belon³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To evaluate intestinal inflammatory and apoptotic processes after intestinal ischemia/reperfusion injury, modulated by pentoxifylline and hypertonic saline. METHODS:It was allocated into four groups (n=6), 24 male Wistar rats (200 to 250g) and submitted to intestinal ischemia for 40 min and reperfusion for 80 min: IR (did not receive any treatment); HS group (Hypertonic Saline, 4ml/kg-IV); PTX group (Pentoxifylline, 30mg/ kg-IV); HS+PTX group (Hypertonic Saline and Pentoxifylline). All animals were heparinized (100U/kg). At the end of reperfusion, ileal fragments were removed and stained on hematoxylin-eosin and histochemical studies for COX-2, Bcl-2 and cleaved caspase-3. RESULTS:The values of sO 2 were higher on treated groups at 40 minutes of reperfusion (p=0.0081) and 80 minutes of reperfusion (p=0.0072). Serum lactate values were lower on treated groups after 40 minutes of reperfusion (p=0.0003) and 80 minutes of reperfusion (p=0.0098). Morphologic tissue injuries showed higher grades on IR group versus other groups: HS (p=0.0006), PTX (p=0.0433) and HS+PTX (p=0.0040). The histochemical study showed lesser expression of COX-2 (p=0.0015) and Bcl-2 (p=0.0012) on HS+PTX group. A lower expression of cleaved caspase-3 was demonstrated in PTX (p=0.0090; PTXvsIR). CONCLUSION:The combined use of pentoxifylline and hypertonic saline offers best results on inflammatory and apoptotic inhibitory aspects after intestinal ischemia/reperfusion.

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Section: Discussionmentioning

confidence: 99%

Pentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats

Marques

Rasslan²,

Belon³

et al. 2014

Acta Cir. Bras.

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“…Intestinal ischemia-reperfusion injury (IRI) is a major and frequent clinical problem in perioperative settings such as vascular surgery, 1 trauma, 2 and liver 3 and intestinal 4 transplantation. In addition, acute mesenteric ischemia is a dire surgical emergency with a mortality rate approaching 60–90%.…”

Section: Introductionmentioning

confidence: 99%

Isoflurane Post-conditioning Protects Against Intestinal Ischemia-Reperfusion Injury and Multiorgan Dysfunction via Transforming Growth Factor-β1 Generation

et al. 2012

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Objective This study examined volatile anesthetic-mediated protection against intestinal ischemia-reperfusion injury (IRI). Background Intestinal IRI is a devastating complication in the perioperative period leading to systemic inflammation and multi-organ dysfunction. Volatile anesthetics, including isoflurane, have anti-inflammatory effects. We aimed to determine whether isoflurane, given after intestinal ischemia, protects against intestinal IRI and the mechanisms involved in this protection. Methods After IACUC approval, mice were anesthetized with pentobarbital and subjected to 30 min of superior mesenteric artery ischemia, followed by 4 hrs of equianesthetic doses of pentobarbital or isoflurane. Five hrs after reperfusion, small intestine tissues were analyzed for morphological injury, apoptosis, neutrophil infiltration, pro-inflammatory mRNAs, and TGF-β1 levels. We also assessed hepatic and renal injury after intestinal IRI. Results Intestinal IRI with pentobarbital led to significant small intestinal dysfunction with increased mucosal injury, TUNEL-positive cells, neutrophil infiltration, and pro-inflammatory mRNAs as well as elevated plasma alanine aminotransferase and creatinine levels. Isoflurane exposure after IRI led to significant attenuation of intestinal, hepatic, and renal injuries. Furthermore, the protective effects of isoflurane were abolished by treatment with a TGF-β1 neutralizing antibody prior to induction of IRI. Finally, isoflurane exposure led to increased TGF-β1 levels in intestinal epithelial cells and in plasma. Conclusions Our findings demonstrate that isoflurane postconditioning protects against small intestinal injury as well as hepatic and renal dysfunction after severe intestinal IRI via induction of intestinal epithelial TGF-β1. Our findings support therapeutic applications of volatile anesthetics during the intraoperative as well as postoperative periods and imply an important role of TGF-β1 signaling in modulating multi-organ injury.

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“…[5] The main causes of portal venous occlusion are liver transplantation and liver surgery with PV occlusion (Pringle maneuver) or PV reconstruction following trauma or extensive cancer surgery. [6] Histopathological assessment as a ''gold standard'' for definitive assessment of the intestinal injury severity is time consuming and is unable to quantify exactly the degree of injury. [7] The significance of this work is a pilot correlation of histopathological assessment of the intestine injury with the fluorescence analysis.…”

Section: Introductionmentioning

confidence: 99%

Detection of Intestinal Ischemia-Reperfusion Injury by Fluorescence Analysis of Intestinal Samples

Tóth

Pekárová

Tomečková

et al. 2013

Spectroscopy Letters

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The aim of this study was to evaluate endogenous fluorescence of small intestine samples and compare them with the histopathological results. The autofluorescence of intestinal homogenates and histopathological injury index after 1h of mesenteric or portal ischemia, and 1 or 24 hours of reperfusion was analysed. Our results revealed two maximum peaks on the EEM. Increase of the intestinal histopathological injury index was accompanied with significant increase of fluorescence intensities in both zones. Close correlation between autofluorescence and histopathological results was found. Autofluorescence can be used was revealed suggesting use of autofluorescence as a new diagnostic method of intestinal injury.

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Induction of intestinal ischemia reperfusion injury by portal vein outflow occlusion in rats

Cited by 20 publications

References 20 publications

Pentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats

Pentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats

Isoflurane Post-conditioning Protects Against Intestinal Ischemia-Reperfusion Injury and Multiorgan Dysfunction via Transforming Growth Factor-β1 Generation

Detection of Intestinal Ischemia-Reperfusion Injury by Fluorescence Analysis of Intestinal Samples

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