Induction of Interferon Production by Conformation-Modified Proteins of Plasma γ-Globulin Fraction

Cheknyov, S. B.; Babayants, A. A.; Денисова, Е. А.

doi:10.1007/s10517-009-0349-x

Cited by 3 publications

(14 citation statements)

References 9 publications

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“…The same regularities were observed under conditions of late induction with predominant production of IFN-γ, when zinc reduced and copper stimulated the manifestation of IFN-inducing activity of γ-globulin, binding these cations. In parallel, zinc lost its own IFN inductor potential, while copper (presumably) realizes its potential even as a component of the protein-metal complex [4].…”

Section: Resultsmentioning

confidence: 99%

“…The content of IFN-α in supernatants of induced leukocytes reached 60-90 pg/ml and correlated with antiviral activity of the samples. Zinc bound to human serum γ-globulin attenuated and copper stimulated the realization of IFN-inducing characteristics of the protein at early terms of incubation.Previous studies showed that the pool of IFN produced in the presence of plasma γ-globulin fraction proteins contains mainly the cytokine corresponding to IFN-γ by its properties [4]. Metal-specific conformation changes of Fc region of the antibody molecule caused by incorporation of copper or zinc cations [5] potentiate or attenuate the effect of IFN induction by the protein which bound the metal and was modified by it [4].…”

mentioning

confidence: 99%

“…Previous studies showed that the pool of IFN produced in the presence of plasma γ-globulin fraction proteins contains mainly the cytokine corresponding to IFN-γ by its properties [4]. Metal-specific conformation changes of Fc region of the antibody molecule caused by incorporation of copper or zinc cations [5] potentiate or attenuate the effect of IFN induction by the protein which bound the metal and was modified by it [4].…”

mentioning

confidence: 99%

“…Metal-specific conformation changes of Fc region of the antibody molecule caused by incorporation of copper or zinc cations [5] potentiate or attenuate the effect of IFN induction by the protein which bound the metal and was modified by it [4].…”

mentioning

confidence: 99%

“…On the other hand, it manifests in induction of gene transcription and production of IFN-γ upon FcR binding to Fc fragment of IgG [9]. Realization of these interactions in an experimental system approximating the physiological system by a number of properties, suggested that conformation changes of Fc fragments of antibody molecule caused by binding of metal cations serve as a natural factor regulating intracellular signaling triggered by activated FcR and normally inducing the production of IFN-γ [4,5].…”

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confidence: 99%

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Detection of IFN-α Produced in the Presence of Plasma γ-Globulin Fraction Proteins

et al. 2009

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Interferon-alpha was detected in IFN pool produced by human leukocytes in the presence of gamma-globulin fraction proteins, copper and zinc cations, and metal-modified gamma-globulins. The cytokine appeared in culture medium at early terms (24 h) of incubation, is characterized by acid resistance, and is neutralized by antibodies to IFN-alpha. The content of IFN-alpha in supernatants of induced leukocytes reached 60-90 pg/ml and correlated with antiviral activity of the samples. Zinc bound to human serum gamma-globulin attenuated and copper stimulated the realization of IFN-inducing characteristics of the protein at early terms of incubation.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Detection of IFN-α Produced in the Presence of Plasma γ-Globulin Fraction Proteins

et al. 2009

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Production of Late IFN-α Induced by Plasma γ-Globulin Fraction Proteins and Their Metal Complexes

et al. 2011

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Plasma γ-globulin fraction proteins, copper and zinc cations, and metal complexes they form with human serum γ-globulin induce the production of IFN-α by human blood cells throughout the periods of up to 72 h. Zinc cation-modified protein by 1.6 times (p<0.05) more actively induces late IFN-α than the control γ-globulin; γ-globulin-copper metal complex is 2-fold (p<0.002) more effective than the control protein. The results indicate that functional relationships between the components inducing the production of late IFN-α differ from the effects realized during the early period of induction.

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The Enhancing Effects of 10% PBS Washout of Holocene Minerals on HuIFN-αN3 Inducing Capacity of NDV ZG1999HDS or Sendai virus (Cantell Strain)

Filipič¹,

Gradišnik

Pereyra³

et al. 2022

Life

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Different strains of Newcastle disease viruses (NDV) or Sendai viruses (SV) are used to induce the production of human leukocyte multi subtype interferon-alpha (HuIFN-αN3). Their inducing capacity can be enhanced in different ways. One includes 10% PBS washout of Holocene minerals (HM). The presented study aims to compare the HuIFN-αN3 inducing capacity of NDV ZG1999HDS or SV (Cantell strain) strain in vitro, and to evaluate the enhancing effect of 10% PBS washouts of HM on both viruses. The NDV strains’ ZG1999HDS interferon inducing capacity (483.23 ± 4.5 pg/mL) was similar to that of the SV (Cantell strain) (584.16 ± 5.9 pg/mL). It was shown that the HuIFN-αN3 inducing capacity of the strain of NDV ZG1999HDS can be strongly enhanced with 10% PBS washout of HM to 3818.21 ± 41.9 pg/mL and 4790.34 ± 33.5 pg/mL with SV (Cantell strain), u. The RP-HPLC analyses of such HuIFN-αN3 induced with the strain of NDV ZG1999HDS show the difference to SV (Cantell strain) induced HuIFN-αN3 in the absence of subtype α14 and the lower level of the subtype α1. The possible ways of such enhancement were also studied and it was postulated that the Fe2+ ions from 10% PBS washouts of HM, while stimulating the reactive oxygen species (ROS) and nitric oxide (NO) formation, activate the transcription factor NF- κB and consequently the production of HuIFN-αN3.

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Induction of Interferon Production by Conformation-Modified Proteins of Plasma γ-Globulin Fraction

Cited by 3 publications

References 9 publications

Detection of IFN-α Produced in the Presence of Plasma γ-Globulin Fraction Proteins

Detection of IFN-α Produced in the Presence of Plasma γ-Globulin Fraction Proteins

Production of Late IFN-α Induced by Plasma γ-Globulin Fraction Proteins and Their Metal Complexes

The Enhancing Effects of 10% PBS Washout of Holocene Minerals on HuIFN-αN3 Inducing Capacity of NDV ZG1999HDS or Sendai virus (Cantell Strain)

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