Induction of immune-related gene expression by seminal exosomes in the porcine endometrium

Bai, Rulan; Latifi, Zeinab; Kusama, Kaoru; Nakamura, Keigo; Shimada, M.; Imakawa, Kazuhiko

doi:10.1016/j.bbrc.2017.11.100

Cited by 61 publications

(54 citation statements)

References 32 publications

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“…These small intraluminal vesicles fuse with the plasma membrane of target cells when released into the extracellular environment ( 7 ). Exosomes can be detected in all bodily fluids, such as urine ( 8 ), seminal fluid ( 9 ), blood ( 10 ) and amniotic fluids ( 11 ). In the transmission of information and regulation of cell signal transduction between different cells, exosomes serve a significant role by selectively delivering biologically active substances to the target cells ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

A highly efficient method for isolating urinary exosomes

Zhu

Wang

et al. 2018

Int J Mol Med

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In the present study, a highly efficient method, referred to as optimized ultrafiltration (OUF), was developed. This method is effective for exosome purification and also facilitates clinical work involving substantial urinary exosome isolation. In the OUF method, 0.22-µm filters along with a dialysis membrane with a molecular weight cut-off of 10,000 kDa were introduced, in order to remove extracellular microvesicles that were >200 nm and concentrate the supernatant up to 1/50 of the initial volume. The existence, purity and production of the exosomes isolated by OUF and conventional ultracentrifugation (UC) were systematically compared by transmission electron microscopy, western blotting and nanoparticle tracking analysis. In addition, colloidal Coomassie-stained gel and reverse transcription-quantitative polymerase chain reaction were used to investigate the stability and integrity of exosomes isolated by these two protocols. The time required and cost of these two methods in the process of isolating urinary exosomes were also estimated. The results indicated that OUF clearly outperforms UC in quantity, quality and biological stability, and this improved method may have extensive applications in the growing fields of clinical biomarker discovery and exosome research.

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Section: Introductionmentioning

confidence: 99%

A highly efficient method for isolating urinary exosomes

Zhu

Wang

et al. 2018

Int J Mol Med

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“…Among the many methods applied in different fluids and species (reviewed by Konoshenko, Lekchnov, Vlassov, & Laktionov, ) to isolate EVs (microvesicles and/or exosomes), immunoaffinity precipitation and capture is the method most often commercially offered (Exo‐Quick‐TC; System Biosciences) as being easy to perform and quick (Bai et al, ). However, due to the low density and small size of the exosomes, purity is low‐to‐poor and demands further screening of other exosome surface markers, such as CD44 for example (Alvarez‐Rodriguez, Ljunggren, et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…However, not only do the EVs carry ncRNAs, and specific proteins; lipoproteins are also carriers with similar delivery capacity for miRNAs, both low‐density (LDL) and particularly high‐density lipoproteins (HDL) (Vickers, Palmisano, Shoucri, Shamburek, & Remaley, ). Since exosomes are comprised to a large extent by membrane lipids, further studies are needed to aid in the more accurate isolation and classification of the different EVs (Piehl et al, ; Skotland, Hessvik, Sandvig, & Llorente, ) and to determine their eventual protective role on chicken spermatozoa (Atikuzzaman, Alvarez‐Rodriguez, et al, ) and its signalling for already documented changes in gene expression by the female genital tract, including immune process genes (Alvarez‐Rodriguez, Atikuzzaman, et al, ; Bai et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Samples were explored for presence, aspect and distribution frequency using electron microscopy (EM), FC (incubated with an antibody against CD9) and WB (using an antibody against the membrane receptor CD44). Boar isolate EVs (microvesicles and/or exosomes), immunoaffinity precipitation and capture is the method most often commercially offered (Exo-Quick-TC; System Biosciences) as being easy to perform and quick (Bai et al, 2018). However, due to the low density and small size of the exosomes, purity is low-to-poor and demands further screening of other exosome surface markers, such as CD44 for example (Alvarez-Rodriguez, Ljunggren, et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…In the mammalian SP, the EVs are classified by size, from those as small (30-100 nm) as exosomes to those as large (100-1,000 nm) as microvesicles (Aalberts, Stout, & Stoorvogel, 2014;Machtinger, Laurent, & Baccarelli, 2015). The exosomes are also classified by point of origin, such as epididymosomes, prostasomes or even vesiculosomes (Bai et al, 2018;Höög & Lötvall, 2015;Sullivan & Saez, 2013), and they interact with both spermatozoa and the internal epithelial lining of the female genital tract (Bai et al, 2018;Du et al, 2016). Sperm modifications after spermatogenesis are caused by, amongst other factors, by EVs trafficking components to the spermatozoa (Girouard, Frenette, & Sullivan, 2009).…”

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confidence: 99%

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Chicken seminal fluid lacks CD9‐ and CD44‐bearing extracellular vesicles

Álvarez‐Rodríguez

Ntzouni

Wright

et al. 2020

Reprod Domestic Animals

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The avian seminal fluid (SF) is a protein‐rich fluid, derived from the testis, the rudimentary epididymis and, finally, from the cloacal gland. The SF interacts with spermatozoa and the inner cell lining of the female genital tract, to modulate sperm functions and female immune responsiveness. Its complex proteome might either be free or linked to extracellular vesicles (EVs) as it is the case in mammals, where EVs depict the tetraspanin CD9; and where those EVs derived from the epididymis (epididymosomes) also present the receptor CD44. In the present study, sperm‐free SF from Red Jungle Fowl, White Leghorn and an advanced intercross (AIL, 12th generation) were studied using flow cytometry of the membrane marker tetraspanin CD9, Western blotting of the membrane receptor CD44 and electron microscopy in non‐enriched (whole SF) or enriched fractions obtained by precipitation using a commercial kit (Total Exosome Precipitation Solution). Neither CD9‐ nor CD44 could be detected, and the ultrastructure confirmed the relative absence of EVs, raising the possibility that avian SF interacts differently with the female genitalia as compared to the seminal plasma of mammals.

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Exosome‐based intercellular communication in female reproductive microenvironments

Ranjbaran

Latifi

Nejabati

et al. 2019

Journal Cellular Physiology

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Different strategies are applied for cellular cross-talk and organization in multicellular organisms. Exosomes are a homogenous population of biological nanoparticles (30-100 nm), originated from multivesicular bodies. The exosomes (Exos) could regulate and affect both cellular physiology and pathophysiology in various organs, such as the female reproductive tract, by altering gene pathways and/or epigenetic programming. Besides, engineered Exos have the potential to be used as a novel drug and gene delivery tools. Here in this review, we discussed various aspects of exosome-based intercellular communication in female reproductive microenvironments. Furthermore, we addressed the findings and issues related to Exos in reproductive biology to give a better view of the involved molecular mechanisms. Moreover, clinical applications of the Exos and their isolation source/methods have been considered to throw some light on the progression of new biological, diagnostic, and therapeutic approaches in clinical embryology. K E Y W O R D S cell communication, embryology, exosome, female reproduction

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Induction of immune-related gene expression by seminal exosomes in the porcine endometrium

Cited by 61 publications

References 32 publications

A highly efficient method for isolating urinary exosomes

A highly efficient method for isolating urinary exosomes

Chicken seminal fluid lacks CD9‐ and CD44‐bearing extracellular vesicles

Exosome‐based intercellular communication in female reproductive microenvironments

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