Induction of IL-8 production in human alveolar macrophages and human bronchial epithelial cells in vitro by swine dust

Palmberg, Lena; Larsson, Britt‐Marie; Malmberg, Per; Larsson, Kjell

doi:10.1136/thx.53.4.260

Cited by 99 publications

(90 citation statements)

References 28 publications

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“…Endotoxin has been demonstrated to increase airway epithelial cell IL-8 expression cells (25,26). In the latter studies, LPS concentrations of 10 -100 g/mL were used.…”

Section: Discussionmentioning

confidence: 99%

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Interleukin (IL)-1β in Tracheal Aspirates from Premature Infants Induces Airway Epithelial Cell IL-8 Expression via an NF-κB Dependent Pathway

et al. 2004

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Tracheal aspirate IL-8 concentration and airway epithelial cell IL-8 expression are each increased in premature infants undergoing mechanical ventilation. We sought to determine the cytokines responsible for IL-8 expression in this context. Tracheal aspirates were collected from 18 mechanically ventilated premature infants. IL-8 protein abundance was high in tracheal aspirates from ventilated premature infants (mean, 5806 Ϯ 4923 pg/mL). IL-1␣ (mean, 20 Ϯ 6 pg/mL), IL-1␤ (mean 67 Ϯ 46 pg/mL), and tumor necrosis factor (TNF)-␣ (mean, 8 Ϯ 2 pg/mL) were also found. Incubation of tracheal aspirates with 16HBE14o-human bronchial epithelial cells increased IL-8 protein in both cell lysates and supernatants, as well as transcription from the IL-8 promoter. Aspirates also induced nuclear factor (NF)-B activation. Mutation of the IL-8 promoter NF-B site abolished aspirate-induced IL-8 transcription. Endotoxin concentrations in the tracheal aspirates were negligible and incapable of inducing IL-8 promoter activity. Finally, incubation of tracheal aspirates with a neutralizing antibody against IL-1␤ reduced epithelial cell IL-8 production, whereas neutralizing antibodies against IL-1␣ and TNF-␣ had no effect. We conclude that airway fluid from mechanically ventilated premature infants contains soluble factors capable of inducing airway epithelial cell IL-8 expression via a NF-B-dependent pathway, and that IL-1␤ plays a specific role in this process. Despite advances in neonatal care, BPD continues to be a major cause of morbidity and mortality in premature neonates. Accordingly, interest remains high in the pathogenesis of this chronic lung disease. Recent evidence suggests the importance of inflammation in the pathogenesis of BPD. During the first days of postnatal life, an influx of polymorphonuclear leukocytes and macrophages is seen in the tracheal aspirates of mechanically ventilated premature infants who later develop BPD (1). Elevations of pro-inflammatory cytokines such as IL-8, IL-6, IL-1␤, and TNF-␣ are also seen (2-7). Similar findings are present in animal models of BPD (8) and hyperoxic exposure (9). Of the cytokines implicated in BPD, IL-8 may be of special importance, as it is the most potent neutrophil chemotactic factor.IL-8 may be elaborated by macrophages, T-lymphocytes, neutrophils, and respiratory epithelial cells (4). In a recent study examining IL-8 expression in the lung tissue of neonates with hyaline membrane disease, the majority of cases demonstrated IL-8 immunoreactivity in fetal and neonatal neutrophils, and in almost half of these cases, airway epithelial cell IL-8 expression (10). An earlier study in ventilated preterm infants detected IL-8 in bronchoalveolar macrophages, neutrophils, and exfoliated epithelial cells (6). In newborn rats, hyperoxic exposure induced the expression of cytokineinduced neutrophil chemoattractant-1, an IL-8 homologue, in alveolar macrophages and epithelial cells (9). Numerous studies have shown that cultured airway epithelial cells may produce IL-8 in response to a...

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“…Endotoxin has been demonstrated to increase airway epithelial cell IL-8 expression cells (25,26). In the latter studies, LPS concentrations of 10 -100 g/mL were used.…”

Section: Discussionmentioning

confidence: 99%

“…Endotoxin has been reported to increase IL-8 expression in airway epithelial cells (25,26). Endotoxin concentrations of tracheal aspirates from three premature infants with respiratory disease were measured by Limulus Amebocyte Lysate assay.…”

Section: Tracheal Aspirate Il-8 Il-1␣ Il-1␤mentioning

confidence: 99%

Interleukin (IL)-1β in Tracheal Aspirates from Premature Infants Induces Airway Epithelial Cell IL-8 Expression via an NF-κB Dependent Pathway

et al. 2004

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“…Acute and chronic exposures of experimental animals to animal facility dust are associated with recruitment of neutrophils and macrophages into the lung and lung pathology similar to that in humans exposed to animal facility dust (9,16,43). In vitro exposure of macrophages and lung epithelial cells to animal facility and agricultural dust extracts increases production of proinflammatory cytokines interleukin-6 (IL-6) and IL-8 (40,46,61).…”

mentioning

confidence: 99%

Transcriptional mechanisms and protein kinase signaling mediate organic dust induction of IL-8 expression in lung epithelial and THP-1 cells

Gottipati

Bandari

Nonnenmann

et al. 2015

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Increased concentrations of TNF-a, IL -1, IL-6 and IL-8 were also found in BAL fluid following exposure to swine dust in vivo [3,4] and in supernatant of epithelial cells and alveolar macrophages following exposure to dust in vitro [9,10]. Granulocytes have also been shown to release many of these cytokines, i.e.…”

Section: Discussionmentioning

confidence: 89%

Lymphocyte activation after exposure to swine dust: a role of humoral mediators and phagocytic cells

Müller-Suur¹,

Larsson²,

Larsson³

et al. 2002

Eur Respir J

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Induction of IL-8 production in human alveolar macrophages and human bronchial epithelial cells in vitro by swine dust

Cited by 99 publications

References 28 publications

Interleukin (IL)-1β in Tracheal Aspirates from Premature Infants Induces Airway Epithelial Cell IL-8 Expression via an NF-κB Dependent Pathway

Interleukin (IL)-1β in Tracheal Aspirates from Premature Infants Induces Airway Epithelial Cell IL-8 Expression via an NF-κB Dependent Pathway

Transcriptional mechanisms and protein kinase signaling mediate organic dust induction of IL-8 expression in lung epithelial and THP-1 cells

Lymphocyte activation after exposure to swine dust: a role of humoral mediators and phagocytic cells

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