Induction of IL-6 and IL-8 by activation of thermosensitive TRP channels in human PDL cells

Son, Ga Yeon; Hong, Jeong Hee; Chang, Inik; Shin, Dong Min

doi:10.1016/j.archoralbio.2014.12.014

Cited by 21 publications

(18 citation statements)

References 18 publications

(21 reference statements)

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“…It also demonstrated a full agonist activity at GPR18 when screened in its ability to activate p‐ERK (EC 50 3.83 µM) 88 . Additionally, AEA also activates the vanilloid (TRPV1) receptor (potency of 0.7‐5 µM) 89 to regulate bone metabolism in PD, 90 peroxisome proliferator‐activated receptor‐γ (PPAR) (8 µM), 91,92 which are reported to be expressed in PDL cells 93‐95 . Moreover, AEA directly inhibits voltage‐dependent T‐ Type calcium channels (1 µM), 96 which are involved in the proliferation of hPDLFs via bFGF, 97 and the acid‐sensitive K + channel (TASK‐1) at 3 µM, 98 also expressed in hPDLFs 99 .…”

Section: Discussionmentioning

confidence: 99%

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Abidi

Alghamdi

Dabbous

et al. 2020

J of Periodontal Research

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Objective The aim of this study is to understand the role of cannabinoid type 2 receptor (CB2R) during periodontal inflammation and to identify anti‐inflammatory agents for the development of drugs to treat periodontitis (PD). Background Cannabinoid type 2 receptor is found in periodontal tissue at sites of inflammation/infection. Our previous study demonstrated anti‐inflammatory responses in human periodontal ligament fibroblasts (hPDLFs) via CB2R ligands. Methods Anandamide (AEA), HU‐308 (agonist), and SMM‐189 (inverse agonist) were tested for effects on IL‐1β‐stimulated cytokines, chemokines, and angiogenic and vascular markers expressed by hPDLFs using Mesoscale Discovery V‐Plex Kits. Signal transduction pathways (p‐c‐Jun, p‐ERK, p‐p‐38, p‐JNK, p‐CREB, and p‐NF‐kB) were investigated using Cisbio HTRF kits. ACTOne and Tango™ ‐BLA functional assays were used to measure cyclic AMP (cAMP) and β‐arrestin activity. Results IL‐1β stimulated hPDLF production of 18/39 analytes, which were downregulated by the CB2R agonist and the inverse agonist. AEA exhibited pro‐inflammatory and anti‐inflammatory effects. IL‐1β increased phosphoproteins within the first hour except p‐JNK. CB2R ligands attenuated p‐p38 and p‐NFĸB, but a late rise in p‐38 was seen with HU‐308. As p‐ERK levels declined, a significant increase in p‐ERK was observed later in the time course by synthetic CB2R ligands. P‐JNK was significantly affected by SMM‐189 only, while p‐CREB was elevated significantly by CB2R ligands at 180 minutes. HU‐308 affected both cAMP and β‐arrestin pathway. SMM‐189 only stimulated cAMP. Conclusion The findings that CB2R agonist and inverse agonist may potentially regulate inflammation suggest that development of CB2R therapeutics could improve on current treatments for PD and other oral inflammatory pathologies.

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Section: Discussionmentioning

confidence: 99%

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Abidi

Alghamdi

Dabbous

et al. 2020

J of Periodontal Research

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“…[29][30][31] Immune functions have been associated with this sensor and some of these have been linked to the central nervous system. 32,33 It is possible that this temperature sensor in the body, if dysfunctional, is associated with the incidence of MS reported in the world and may explain temperature dependent incidences and prevalences of multiple sclerosis. The reason why variation towards a particular temperature would affect the results is presumably because this is roughly the point at which the TRPM8 receptor would cease to be reactive and crossing this point would cause prevalence rates to rise or fall.…”

Section: Discussionmentioning

confidence: 99%

Multiple sclerosis prevalence in Italy associated with temperature variation towards 8.60°C

Heisten¹

2018

Pol. Ann. Med.

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I n t r o d u c t i o n : Italy is an exception to the association of increasing Latitudinal prevalence of multiple sclerosis (MS) with temperature. This is noteworthy because even though northern Italy's average yearly temperature is significantly colder than southern Italy's MS rates are lower instead of higher. If the reason for this inverse epidemiology of MS were determined it would give important insight as to the causes of MS. A i m : This study was done to determine if variation towards a particular temperature was responsible for MS prevalence rates in Italy.

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“…Moreover, Ca 2+ signals modulate inflammatory and immune responses in different tissues and cells, including dental pulp, hPDLCs, and human gingival fibroblasts (Colotta, Jansson, & Bonelli, ; G. Y. Son, Hong, Chang, & Shin, ; Szymanski et al, ; Tanaka et al, ). In the study of the immune defense mechanisms in dental pulp, particular attention has been paid to odontoblasts that are situated at the pulp–dentin interface and that constitute the first line of defense against cariogenic bacteria entering dentin after enamel disruption (Farges et al, ; Farges et al, ).…”

Section: Cellular Responses To Extracellular Ca2+ and The Signaling Pmentioning

confidence: 99%

The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

2018

Journal Cellular Physiology

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Calcium ions (Ca ) is the main element of dental pulp capping materials. Ca signaling plays a crucial role in a myriad of cell activities. An overwhelming array of studies have already reported the experimental and clinical benefits of Ca -enriched materials in the treatment of teeth with accidental vital pulp exposure and incomplete root formation. Thus, Ca signaling has always been an excellent target for the design of various novel biomaterials for use in revitalizing or regenerative endodontic procedures. However, the molecular mechanisms that enable dental pulp cells (DPCs) to detect and respond to extracellular Ca have not been characterized in detail before. In this review, we mainly outline the pathways by which the cell detects and responds to extracellular Ca , as well as the relevant regulatory paths in DPCs and odontoblasts, and discuss the potential role of Ca as a therapeutic tool. Moreover, because DPCs share many of the same functional properties that are found in osteoblasts, some comparisons with bone cells were additionally incorporated into this text.

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Induction of IL-6 and IL-8 by activation of thermosensitive TRP channels in human PDL cells

Cited by 21 publications

References 18 publications

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Multiple sclerosis prevalence in Italy associated with temperature variation towards 8.60°C

The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

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Induction of IL-6 and IL-8 by activation of thermosensitive TRP channels in human PDL cells

Cited by 21 publications

References 18 publications

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Cannabinoid type‐2 receptor agonist, inverse agonist, and anandamide regulation of inflammatory responses in IL‐1β stimulated primary human periodontal ligament fibroblasts

Multiple sclerosis prevalence in Italy associated with temperature variation towards 8.60°C

The emerging role of extracellular Ca2+ in osteo/odontogenic differentiation and the involvement of intracellular Ca2+ signaling: From osteoblastic cells to dental pulp cells and odontoblasts

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The emerging role of extracellular Ca²⁺ in osteo/odontogenic differentiation and the involvement of intracellular Ca²⁺ signaling: From osteoblastic cells to dental pulp cells and odontoblasts