Induction of human IgE synthesis in B cells by mast cells and basophils

Gauchat, J F; Henchoz, Sybille; Mazzei, Gonzalo J.; Aubry, J. P.; Brunner, Thomas; Blasey, Horst; Life, Paul; Talabot, Dominique; Flores‐Romo, Leopoldo; Thompson, Jeffrey P.; Kishi, Kenji; Butterfield, Joseph H.; Dahinden, Clemens A.; Bonnefoy, Jean Yves

doi:10.1038/365340a0

Cited by 599 publications

(343 citation statements)

References 29 publications

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“…We therefore also examined the expression of TNF-TNFR family molecules on BMCMCs. It has been reported that human mast cell lines and tissue mast cells can express CD40L (CD154), and mast cell-derived CD40L is thought to contribute to IgE production by B cells (38,39). However, we did not detect any CD40L expression on BMCMCs in our experiments (Fig.…”

Section: Expression Of Members Of the Tnf And Tnfr Families Of Molecucontrasting

confidence: 62%

Mast Cells Enhance T Cell Activation: Importance of Mast Cell Costimulatory Molecules and Secreted TNF

Nakae

Suto

Iikura

et al. 2006

The Journal of Immunology

353

306

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We recently reported that mast cells stimulated via FcεRI aggregation can enhance T cell activation by a TNF-dependent mechanism. However, the molecular mechanisms responsible for such IgE-, Ag- (Ag-), and mast cell-dependent enhancement of T cell activation remain unknown. In this study we showed that mouse bone marrow-derived cultured mast cells express various costimulatory molecules, including members of the B7 family (ICOS ligand (ICOSL), PD-L1, and PD-L2) and the TNF/TNFR families (OX40 ligand (OX40L), CD153, Fas, 4-1BB, and glucocorticoid-induced TNFR). ICOSL, PD-L1, PD-L2, and OX40L also are expressed on APCs such as dendritic cells and can modulate T cell function. We found that IgE- and Ag-dependent mast cell enhancement of T cell activation required secreted TNF; that TNF can increase the surface expression of OX40, ICOS, PD-1, and other costimulatory molecules on CD3+ T cells; and that a neutralizing Ab to OX40L, but not neutralizing Abs to ICOSL or PD-L1, significantly reduced IgE/Ag-dependent mast cell-mediated enhancement of T cell activation. These results indicate that the secretion of soluble TNF and direct cell-cell interactions between mast cell OX40L and T cell OX40 contribute to the ability of IgE- and Ag-stimulated mouse mast cells to enhance T cell activation.

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Section: Expression Of Members Of the Tnf And Tnfr Families Of Molecucontrasting

confidence: 62%

Mast Cells Enhance T Cell Activation: Importance of Mast Cell Costimulatory Molecules and Secreted TNF

Nakae

Suto

Iikura

et al. 2006

The Journal of Immunology

353

306

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“…Similar observations of isotype switching have been made by SAXON et al [22] in nasal mucosal cells. Not only T-cells but also mast cells and basophils have the capacity to stimulate IgE synthesis by B-cells, as producers of IL-4 and IL-13 and by the interaction of mast cells CD154 (CD40 ligand) with B-cell CD40 [18,30,31]. The mast cell/basophilic induction and stimulation of B-cell IgE-production indicate that immunoglobulin switching, previously thought to take place only in lymph node germinal centres, may also occur in peripheral organs such as the nose.…”

Section: Discussionmentioning

confidence: 99%

Local production and detection of (specific) IgE in nasal B-cells and plasma cells of allergic rhinitis patients

et al. 2000

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Allergic diseases are characterized by allergic complaints in the shock organ and specific immunoglobulin (Ig)E in serum. Literature data indicate that the nasal mucosa itself could produce at least a large part of the specific IgE in allergic rhinitis patients.In order to investigate this hypothesis, nasal mucosal biopsies from the inferior turbinate were taken from symptomatic grass pollen allergic rhinitis patients, symptomatic house dust mite allergic rhinitis patients and nonallergic healthy controls, confirmed by radioallergosorbent test and skin-prick test. Immunohistochemical double-staining was performed for B-cells (CD19) with IgE, plasma cells (CD138) with IgE and plasma cells with biotinylated allergens.Significantly more IgE-positive B-cells and IgE-positive plasma cells were found in the nasal mucosa of allergic patients than in that of nonallergic controls. Double staining with biotinylated allergens and plasma cells showed allergen-positive plasma cells in the nasal mucosa of allergic patients and no allergen-positive plasma cells in the nasal mucosa of nonallergic patients. Blocking experiments using polyclonal antibodies directed against IgE showed a significant reduction in the number of allergenpositive cells in contrast to experiments using polyclonal antibodies directed against IgG, IgA or IgM.This study describes new evidence that specific immunoglobulin E is produced locally in the nasal mucosa in patients with seasonal allergic rhinitis and perennial allergic rhinitis, but not in nonallergic controls. Eur Respir J 2000; 15: 491±497.

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“…The corresponding ligand CD40L is transiently expressed on activated T cells [28][29][30][31], natural killer (NK) cells [32], mast cells, basophils [33] and eosinophils [34]. CD40 and CD40L are members of the tumour necrosis factor (TNF) receptor ligand family [35,36] and key players in T cell-B cell and T cell-antigen-presenting cell (APC) interaction [37,38].…”

Section: Introductionmentioning

confidence: 99%

Soluble CD40 in the serum of healthy donors, patients with chronic renal failure, haemodialysis and chronic ambulatory peritoneal dialysis (CAPD) patients

Schwabe

Engelmann

Hess

et al. 1999

Clinical and Experimental Immunology

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SUMMARYCD40 and its ligand CD40L are key players in T cell-B cell interaction and T cell-antigen-presenting cell (APC) interaction. Inhibition of CD40-CD40L interaction leads to severe humoral and cellular immunodeficiency. In this study we examined the presence of soluble CD40 (sCD40) in the serum of haemodialysis (HD) patients, CAPD patients, chronic renal failure (CRF) patients and healthy donors in order to evaluate the possible involvement of CD40 in uraemic immunodeficiency. Soluble CD40 was detected in the serum of healthy donors (n ¼ 41) with a mean of 0·14 Ϯ 0·12 ng/ml and in the urine of healthy donors with a mean of 1·80 Ϯ 0·74 ng/ml. Soluble CD40 was highly elevated in all patients with impaired renal function. HD patients (n ¼ 22) had up to 100-fold elevated sCD40 levels with a mean concentration of 8·32 Ϯ 4·11 ng/ml, whereas CAPD patients (n ¼ 10) had considerably lower levels of sCD40 with a mean of 3·58 Ϯ 2·40 ng/ml. A strong correlation between sCD40 and serum creatinine levels was noted in CRF patients (n ¼ 66). The highly elevated levels of sCD40 may point to the involvement of CD40 and its ligand CD40L in the clinical manifestation of uraemic immunodeficiency.

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Induction of human IgE synthesis in B cells by mast cells and basophils

Cited by 599 publications

References 29 publications

Mast Cells Enhance T Cell Activation: Importance of Mast Cell Costimulatory Molecules and Secreted TNF

Mast Cells Enhance T Cell Activation: Importance of Mast Cell Costimulatory Molecules and Secreted TNF

Local production and detection of (specific) IgE in nasal B-cells and plasma cells of allergic rhinitis patients

Soluble CD40 in the serum of healthy donors, patients with chronic renal failure, haemodialysis and chronic ambulatory peritoneal dialysis (CAPD) patients

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