Induction of HLA class‐II antigen expression on human carcinoma cell lines by IFN‐gamma

Schwartz, Reinhard; Momburg, Frank; Moldenhauer, Gerhard; Dörken, Bernd; Schirrmacher, Volker

doi:10.1002/ijc.2910350217

Cited by 75 publications

(21 citation statements)

References 24 publications

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“…The peak fraction was used for the cellular radioimmuno assay (CRIA). The CRIA was performed in flexible polyvinyl chloride microtiter plates, as described previously (Schwartz et al, 1985). Plates were blocked for nonspecific binding by preincubation with PBS + 0.2% (v/v) In order to test for binding specificity of u-PA, representative cell lines of squamous cell carcinoma, large cell carcinoma, SCLC and mesothelioma were incubated with 2 x 106 c.p.m.…”

mentioning

confidence: 99%

Three types of human lung tumour cell lines can be distinguished according to surface expression of endogenous urokinase and their capacity to bind exogenous urokinase

Schwartz‐Albiez

Heidtmann²,

Wolf³

et al. 1992

Br J Cancer

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Summary This study evaluates the cell surface expression of urokinase-type plasminogen activator (u-PA) and the capacity to bind exogenous urokinase as possible parameters for the distinction of various types of human lung tumours. Twelve different tumour cell lines including four small cell carcinoma, two large cell carcinoma, three squamous cell carcinoma, one adenocarcinoma and two mesothelioma cell lines of lung origin were investigated. Surface expression of endogenous u-PA was determined in a cellular radioimmunoassay (CRIA) using the u-PA-specific monoclonal antibody 98/6. To estimate additional u-PA binding capacity, exogenous two-chain, 54 kDa u-PA was employed in the CRIA. The influence of phorbol ester (PMA) treatment on expression and binding of these molecules was studied. Three different groups of lung tumour cell lines could be distinguished according to their expression of u-PA and u-PA-binding ability: (i) non small cell lung carcinoma (NSCLC) cell lines of squamous cell carcinoma/adenocarcinoma origin expressed small amounts of u-PA and bound little u-PA. Large cell carcinoma cell lines expressed high amounts of u-PA and bound large amounts of u-PA. In general, expression of u-PA and u-PA binding was enhanced after PMA treatment. (ii) Mesothelioma cell lines did not express u-PA, but were able to bind u-PA. (iii) Small cell carcinoma (SCLC) lines were devoid of surface-expressed u-PA and could not bind u-PA, both under untreated and PMA-treated conditions. It could thus be demonstrated that these three groups of lung tumour cell lines differ in their ability to express u-PA and to bind external u-PA. This may reflect the different in vivo growth behaviour and origin of the respective tumour groups.

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confidence: 99%

Three types of human lung tumour cell lines can be distinguished according to surface expression of endogenous urokinase and their capacity to bind exogenous urokinase

Schwartz‐Albiez

Heidtmann²,

Wolf³

et al. 1992

Br J Cancer

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“…This indicates that HLA-DR antigens on adenoma and cancer cells may be related to local mononuclear cell infiltration, as well as to the neoplastic change of the epithelia. The association between HLA-DR antigens on cancer cells and infiltrated lymphocytes in the lesions was suggested from the fact that interferon gamma (Carrel et al 1985;Schwartz et al 1985), tumor necrosis factor (Pfizenmaier et al 1987), and other unknown agents produced by human lymphocytes (Groenewegen et al 1986) have the ability to induce expression of HLA-DR antigens on various human cell lines, including human colon cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…Cytokines, such as interferon gamma (Schwartz et al 1985) and tumor necrosis factor (Pfizenmaier et al 1987), that are released from lymphoid cells are known to induce HLA-DR antigens on the colonic cancer cells in vitro. Therefore, mononuclear cell infiltration, capable of releasing such cytokines in the lesion, may be related to the induction of HLA-DR antigens in the neoplastic cells.…”

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confidence: 99%

HLA antigens on colorectal adenoma and cancer cells.

Horie

Chiba

Iizuka

et al. 1990

Tohoku J. Exp. Med.

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“…GarciaEspejo et al [20] suggested that the release of lymphokines by the host immune system could play a role in inducing the expression of HLA-DR, on previously negative cells. Indeed colon carcinoma cell lines can be induced to express HLA-DR upon exposure to interferon [22], which is known to up-regulate class I and class II. In colon cancer HLA-DR appeared mainly in poorly differ entiated tumors [23].…”

Section: Discussionmentioning

confidence: 99%

Expression of HLA Class I and Class II in Gastric Carcinoma in Relation to Pathologic Stage

Klein

Nyska

et al. 1991

Tumor Biol

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The expression of HLA class I and II antigens was studied in 41 patients with gastric cancer and in 2 normal stomachs. The normal gastric epithelia were diffusely stained for class I antigens. No staining was observed for class II. In gastric cancer an overall reduction in staining was observed, which was related to the degree of tumor penetration through the gastric wall. In tumors which penetrated the mucosa and submucosa only, 80–90% of tumor cells were stained for class I, as well as the normal areas surrounding the tumor. On the other hand, in tumors which penetrated through the organ to the fat, only 10–20% of cells were stained. Staining for class II was observed in only 12 cases, all of them advanced, with penetration to muscle or fat. No staining was observed in the normal areas surrounding the tumor. The importance of HLA antigens in immune surveillance is discussed.

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Induction of HLA class‐II antigen expression on human carcinoma cell lines by IFN‐gamma

Cited by 75 publications

References 24 publications

Three types of human lung tumour cell lines can be distinguished according to surface expression of endogenous urokinase and their capacity to bind exogenous urokinase

Three types of human lung tumour cell lines can be distinguished according to surface expression of endogenous urokinase and their capacity to bind exogenous urokinase

HLA antigens on colorectal adenoma and cancer cells.

Expression of HLA Class I and Class II in Gastric Carcinoma in Relation to Pathologic Stage

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