Abstract:ABSTRACT-The hemagglutinating activity (HA) of Edwardsiella tarda, which had been isolated from cultured fish and culture environments, was investigated in relation to NaCl concentration of the growth medium. E. tarda cells were cultured in a peptone-yeast extract broth supplemented with 3% NaCl (3%-NaCl culture) and without NaCl (0%-NaCl culture). Hemagglutination assays with guinea pig erythrocytes classified the strains into three HA patterns. Seventeen strains exhibited HA only with the 3%-NaCl culture (ty… Show more
“…In the present study, we demonstrated that the adhesive patterns of E. tarda strains to HEp-2 cells well correlated with their HA patterns against guinea pig erythrocytes described previously, where the HA of E. tarda FK1051 and KG8401 was enhanced by the increase of NaCl concentration in the culture media (Yasunobu et al, 2006). The NaClinduced adherence was also described in Salmonella typhi (Tartera and Metcalf, 1993), in which the adherence ability to Henle 407 epithelial cells increased under higher osmolarity (until 0.3 M NaCl) of the culture medium.…”
Section: Discussionsupporting
confidence: 87%
“…The NaClinduced adherence was also described in Salmonella typhi (Tartera and Metcalf, 1993), in which the adherence ability to Henle 407 epithelial cells increased under higher osmolarity (until 0.3 M NaCl) of the culture medium. Our previous infection experiments with E. tarda FK1051 showed that the 3%-NaCl culture was more virulent to Japanese founder than the 0%-NaCl culture, when fish were challenged orally, and thus we speculated that NaCl-induction of HA is important for adherence of E. tarda to the intestinal epithelial cells of fish (Yasunobu et al 2006). The present result, i.e.…”
Section: Discussionmentioning
confidence: 90%
“…Three strains of E. tarda, representing the different types of HA described previously (Yasunobu et al 2006), were used: strain FK1051 (type A) isolated from diseased Japanese flounder Paralichthys olivaceus, strain KG8401 (type B) from diseased Japanese eel Anguilla japonica, and a non-clinical strain SU166 (type C). These strains were pre-cultured on Trypto-Soya Agar (TSA, Nissui) at 30∞C for 24 h. According to the method described before (Yasunobu et al, 2006), each of the pre-cultured E. tarda strains was inoculated into 10 mL of a liquid medium consisted of 1% peptone-0.5% yeast extract (pH 6.5).…”
Section: Bacterial Strains and Culture Conditionsmentioning
confidence: 99%
“…These strains were pre-cultured on Trypto-Soya Agar (TSA, Nissui) at 30∞C for 24 h. According to the method described before (Yasunobu et al, 2006), each of the pre-cultured E. tarda strains was inoculated into 10 mL of a liquid medium consisted of 1% peptone-0.5% yeast extract (pH 6.5). The bacterial suspension was diluted 100-fold in the same medium, and then 100 mL of the dilution was inoculated into 10 mL of the liquid medium supplemented with 3% NaCl or without NaCl.…”
Section: Bacterial Strains and Culture Conditionsmentioning
confidence: 99%
“…Recently, Sakai et al (2003Sakai et al ( , 2004 reported that a fimbrial major subunit (19.3 kDa protein) is associated with the mannose-resistant hemagglutinating activity (HA) in E. tarda. Furthermore, our previous study revealed that the HA of E. tarda was induced by high (3%) NaCl concentration in the culture medium and E. tarda strains were classified into three types; type A showed HA only with the 3%-NaCl culture, type B exhibited HA in both 0%-and 3%-NaCl cultures, and type C demonstrated no HA in both the cultures (Yasunobu et al, 2006).…”
ABSTRACT-The adherence of Edwardsiella tarda strains to HEp-2 cells was studied by the viable cell count and direct microscopic count methods. Three selected E. tarda strains having different hemagglutination activities against guinea pig erythrocytes were cultured in a peptone-yeast extract broth supplemented with 3% NaCl (3%-NaCl culture) or without NaCl (0%-NaCl culture). Strain FK1051 showed significantly higher adherence in the 3%-NaCl culture than in the 0%-NaCl culture. A similar adherence pattern was observed in strain KG8401, though the adherence of the 0%-NaCl culture was higher than that of FK1051. In contrast, both cultures of strain SU166 were low in the adherence. These adherence patterns to the HEp-2 cells correlated well with the previously reported hemagglutinating activities of the strains. N-acetylneuraminic acid markedly inhibited the adherence as well as the hemagglutination. This NaCl-enhanced adherence may be involved in pathogenesis of E. tarda infection particularly in marine fish.
“…In the present study, we demonstrated that the adhesive patterns of E. tarda strains to HEp-2 cells well correlated with their HA patterns against guinea pig erythrocytes described previously, where the HA of E. tarda FK1051 and KG8401 was enhanced by the increase of NaCl concentration in the culture media (Yasunobu et al, 2006). The NaClinduced adherence was also described in Salmonella typhi (Tartera and Metcalf, 1993), in which the adherence ability to Henle 407 epithelial cells increased under higher osmolarity (until 0.3 M NaCl) of the culture medium.…”
Section: Discussionsupporting
confidence: 87%
“…The NaClinduced adherence was also described in Salmonella typhi (Tartera and Metcalf, 1993), in which the adherence ability to Henle 407 epithelial cells increased under higher osmolarity (until 0.3 M NaCl) of the culture medium. Our previous infection experiments with E. tarda FK1051 showed that the 3%-NaCl culture was more virulent to Japanese founder than the 0%-NaCl culture, when fish were challenged orally, and thus we speculated that NaCl-induction of HA is important for adherence of E. tarda to the intestinal epithelial cells of fish (Yasunobu et al 2006). The present result, i.e.…”
Section: Discussionmentioning
confidence: 90%
“…Three strains of E. tarda, representing the different types of HA described previously (Yasunobu et al 2006), were used: strain FK1051 (type A) isolated from diseased Japanese flounder Paralichthys olivaceus, strain KG8401 (type B) from diseased Japanese eel Anguilla japonica, and a non-clinical strain SU166 (type C). These strains were pre-cultured on Trypto-Soya Agar (TSA, Nissui) at 30∞C for 24 h. According to the method described before (Yasunobu et al, 2006), each of the pre-cultured E. tarda strains was inoculated into 10 mL of a liquid medium consisted of 1% peptone-0.5% yeast extract (pH 6.5).…”
Section: Bacterial Strains and Culture Conditionsmentioning
confidence: 99%
“…These strains were pre-cultured on Trypto-Soya Agar (TSA, Nissui) at 30∞C for 24 h. According to the method described before (Yasunobu et al, 2006), each of the pre-cultured E. tarda strains was inoculated into 10 mL of a liquid medium consisted of 1% peptone-0.5% yeast extract (pH 6.5). The bacterial suspension was diluted 100-fold in the same medium, and then 100 mL of the dilution was inoculated into 10 mL of the liquid medium supplemented with 3% NaCl or without NaCl.…”
Section: Bacterial Strains and Culture Conditionsmentioning
confidence: 99%
“…Recently, Sakai et al (2003Sakai et al ( , 2004 reported that a fimbrial major subunit (19.3 kDa protein) is associated with the mannose-resistant hemagglutinating activity (HA) in E. tarda. Furthermore, our previous study revealed that the HA of E. tarda was induced by high (3%) NaCl concentration in the culture medium and E. tarda strains were classified into three types; type A showed HA only with the 3%-NaCl culture, type B exhibited HA in both 0%-and 3%-NaCl cultures, and type C demonstrated no HA in both the cultures (Yasunobu et al, 2006).…”
ABSTRACT-The adherence of Edwardsiella tarda strains to HEp-2 cells was studied by the viable cell count and direct microscopic count methods. Three selected E. tarda strains having different hemagglutination activities against guinea pig erythrocytes were cultured in a peptone-yeast extract broth supplemented with 3% NaCl (3%-NaCl culture) or without NaCl (0%-NaCl culture). Strain FK1051 showed significantly higher adherence in the 3%-NaCl culture than in the 0%-NaCl culture. A similar adherence pattern was observed in strain KG8401, though the adherence of the 0%-NaCl culture was higher than that of FK1051. In contrast, both cultures of strain SU166 were low in the adherence. These adherence patterns to the HEp-2 cells correlated well with the previously reported hemagglutinating activities of the strains. N-acetylneuraminic acid markedly inhibited the adherence as well as the hemagglutination. This NaCl-enhanced adherence may be involved in pathogenesis of E. tarda infection particularly in marine fish.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.