Induction of fructose 1,6‐bisphosphatase in HL‐60 leukemia cells by retinoic acid

Abstract

The expression of the fructose 1,6‐bisphosphatase gene in HL‐60 cells was induced by retinoic acid. The levels of mRNA, enzyme activity and enzyme protein in the cell line began to rapidly increase after culturing with retinoic acid for 72 h. Retinoic acid dosedependently increased the enzyme activity with maximal stimulation at 1 μM. The responses of the fructose 1,6‐bisphosphatase gene expression by retinoic acid were markedly slower than those of the enzyme expression by 1α,25‐ dihydroxyvitamin D3. When HL‐60 cells were cultured in the presence of both retinoic acid and 1α,25‐dihydroxyvitamin D3, the effects of the two agents on enzyme activity, protein and mRNA were additive.