Induction of food tolerance is dependent on intestinal inflammatory state

Silva, Airton Pereira e; Marmello, Bárbara Oliveira; Soares, João Ricardo Almeida; Mazza-Guimaraes, Isabelle; Teixeira, Gerlinde Agate Platais Brasil

doi:10.1016/j.imlet.2021.04.009

Cited by 2 publications

(4 citation statements)

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“…These antigenspecific memory cells were first described over 20 years ago and are responsible for quick response upon antigen restimulation by switching to a B220 low phenotype [74,75]. This may indicate that EXP-2.3 was sensitized to OVA during the CD, which has been observed in previous studies in our group [32] and also in the literature [76,77]. B220 + CD3 -CD27 + memory B cells are also noteworthy for induction of class-switching to IgG1 in plasma cells through activation of Th2 memory T cells [78], which can explain why EXP-2 and PC showed an increase of this population.…”

Section: Discussionsupporting

confidence: 78%

“…In-house serial dilution enzyme-linked immunosorbent assays (ELISA) were performed to detect specific polyisotypic IgG and IgG1 as previously described [32]. Briefly, after coating 96-well microplates (low binding, Corning, Acton, USA) with 4 μ g of PPE, a 3-fold serial dilution of each serum (starting at 1/100 v/v) was performed.…”

Section: Methodsmentioning

confidence: 99%

“…Negative control (NC) group was kept with mouse chow throughout the experiment [29][30][31]. Food intake was measured three times a week and the mean caloric intake was calculated per gram of body weight (gbw) in each group as previously described [32].…”

Section: Induction Of the Antigen-specific Inflammatory Gut Reactionmentioning

confidence: 99%

“…EXP-1.2 (Experimental 1.2): A hybrid diet (mouse chow and peanuts) was offered. EXP-2.2 (Experimental 2.2): Animals were offered an experimental OVA chow as previously described [32]. OVA chow was used to understand the effect of an unrelated protein used both to induce tolerance or allergy during the treatment period.…”

Section: Experimental Groups and Timelinementioning

confidence: 99%

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Expansion of Antigen- Specific Memory Cells as a Potential Booster for Food Tolerance Induction

Silva¹,

Marmello²,

Bentes³

et al. 2023

Preprint

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Approximately 3% of children in Western countries are diagnosed with peanut allergy, a likely lifelong disease. The preferred treatment for food allergy is allergen avoidance. However, oral immunotherapy is an FDA-approved treatment to re-induce tolerance, still, not all patients respond as expected. Thus, the aim of this work is to evaluate whether the association of an antigen-specific tolerogenic (oral tolerance) bystander effect can ameliorate the recovery of inflamed intestinal mucosa. Adult male C57BL/6 mice were divided into five groups, four of which were submitted to an intestinal inflammation induction protocol to peanuts. After sensitization, experimental groups were orally challenged with either peanuts or a hybrid diet (peanuts + mouse chow). In a second stage, groups were sensitized, challenged with peanuts, and then received either peanuts, hybrid diet, or ovalbumin chow during the recovery period of the inflamed mucosa. Results showed no changes in diet intake and body weight. Polyisotypic anti-peanut IgG and IgG1 were significantly increased in the serum from animals in allergic groups. The group that received the hybrid diet showed an increase in CD4+CD25+Foxp3+ regulatory T cells, as well as in B220+CD3-CD27+ memory B cells. Histology of the duodenum showed a decrease in intraepithelial leukocytes in animals who received hybrid diet. Together, our results show that when the tolerogen is added to a diet containing the allergen, it can ameliorate the induction of local inflammation. Simultaneously offering the allergen with a tolerated food increased the mucosal recovery due to the expansion of previously induced memory cells.

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