Induction of fetal hemoglobin synthesis with recombinant human erythropoietin in anemic patients with heterozygous beta-thalassemia during pregnancy

Breymann, Christian; Fibach, Eitan; Visca, E.; Huettner, Claudia S.; Huch, A.; Huch, R.

doi:10.1002/(sici)1520-6661(199901/02)8:1<1::aid-mfm1>3.0.co;2-o

Cited by 17 publications

(6 citation statements)

References 36 publications

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“…However, in these patients Epo stimulates thalassemic erythropoiesis with production of abnormal RBC having excess α -globin chains, membrane damage, and short survival. As for stimulation of fetal Hb, contradictory results were reported [ 8 , 42 , 43 ]. Our findings raise the possibility that Epo administration may benefit thalassemic patients also by reducing oxidative stress and thereby prolonging the survival of their RBC as well as lowering the state of activation of their platelets.…”

Section: Discussionmentioning

confidence: 99%

The Antioxidant Effect of Erythropoietin on Thalassemic Blood Cells

Amer

Dana

Fibach

2010

Anemia

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Because of its stimulating effect on RBC production, erythropoietin (Epo) is used to treat anemia, for example, in patients on dialysis or on chemotherapy. In β-thalassemia, where Epo levels are low relative to the degree of anemia, Epo treatment improves the anemia state. Since RBC and platelets of these patients are under oxidative stress, which may be involved in anemia and thromboembolic complications, we investigated Epo as an antioxidant. Using flow-cytometry technology, we found that in vitro treatment with Epo of blood cells from these patients increased their glutathione content and reduced their reactive oxygen species, membrane lipid peroxides, and external phosphatidylserine. This resulted in reduced susceptibility of RBC to undergo hemolysis and phagocytosis. Injection of Epo into heterozygous (Hbbth3/+) β-thalassemic mice reduced the oxidative markers within 3 hours. Our results suggest that, in addition to stimulating RBC and fetal hemoglobin production, Epo might alleviate symptoms of hemolytic anemias as an antioxidant.

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Section: Discussionmentioning

confidence: 99%

The Antioxidant Effect of Erythropoietin on Thalassemic Blood Cells

Amer

Dana

Fibach

2010

Anemia

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“…Inappropriately low serum erythropoietin levels may be found in SCD (de Klerk et al 1981) and erythropoietin increases fetal haemoglobin levels (Breymann et al 1999). The increase in both fetal and total haemoglobin makes erythropoietin therapy a useful treatment in SCD.…”

Section: Discussionmentioning

confidence: 99%

Use of erythropoietin in a pregnant Jehovah's Witness with sickle-cell disease

Tan¹,

Ahmad

Jhavar³

et al. 2007

Journal of Obstetrics and Gynaecology

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1999). There is a significantly increased prevalence of tinnitus in pregnant women compared with a similar non-pregnant group (Gurr et al. 1993).There are several speculations why tinnitus occurs in pregnancy. Hyperdynamic circulation, increase in perilymphatic fluid pressure and hormonal changes have been suggested (Preece et al. 1975).Tinnitus could be a warning sign of early pre-eclampsia (Shapiro et al. 1999). A rise in intracranial pressure (resulting from pre-eclampsia) can be transmitted to the perilymphatic fluid through the cochlear duct.We did a Medline search from 1966 to 2005, searching for cases of severe tinnitus in pregnancy, necessitating a caesarean delivery. No case has been reported so far.In this case, normal findings at otoscopy ruled out local pathology as a cause of tinnitus. A normal CT brain scan excluded temporal lobe lesions and glomus tumours of the internal ear. A normal carotid artery ultrasound ruled out a carotid body tumour. Our patient did not have any affective disorder (as is present in 40% of cases with tinnitus) or pre-eclampsia.With all major causes ruled out, we believe that the severe tinnitus was attributable to the pregnancy. We agree with other authors that tinnitus is common in pregnancy, and that complete resolution occurs once pregnancy is over. The uniqueness of our case lies in its severity, i.e. so severe that caesarean delivery was needed. We present this case as an unusual manifestation in pregnancy.

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“…Current Protocols in Cytometry of therapies, such as hydroxyurea, having the specific therapeutic intent of raising F-cell levels (Atweh et al, 1999;Iyamu et al, 2000;. The increase in F-cell or HbF levels parallels an improved clinical course in both sickle cell disease and thalassemia (Breymann et al, 1999;Nagel et al, 1993); however, since the therapies that induce increased F cells and HbF all have toxic side effects, it follows that therapeutic monitoring by F-cell enumeration should facilitate more optimal dosing for these patients. Additionally, F-cell assays could identify patients not requiring such therapies by virtue of preexisting constitutive production of F cells due to their genetic constitution.…”

Section: Of 17mentioning

confidence: 99%

Enumeration of Fetal Red Blood Cells, Hemoglobin‐Specific RBC Cells, and F Reticulocytes in Human Blood

Davis

2019

CP Cytometry

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Recent advances in analytical cytometry have improved diagnostic tools for the study of erythropoiesis in anemic patients and resolution of differential diagnosis in diseases of the erythron. This article presents three applications of red blood cell (RBC) analysis-quantitation of fetal red cells, F-cell enumeration, and F-reticulocyte analysis-which improve diagnostic precision, sensitivity, and specificity, and provide better laboratory indicators of therapeutic efficacy in a variety of hematologic and obstetric disorders. Such advances also include the measurement and quantitation of RBC hemoglobins and their relative ribonucleic acid levels. These advances not only promise to improve diagnostic accuracy and laboratory precision over techniques such as the traditional manual reticulocyte counting method and the Kleihauer-Betke stain method for evaluating fetomaternal hemorrhage (FMH), but also serve as tools for newer assays of anemia diagnosis and improved clinical outcomes. In addition to the primary methods, supporting techniques for preparing spiked controls, automating data analysis, setting up a fetal hemoglobin acquisition protocol, and assaying reticulocytes using thiazole orange are also presented. C 2019 by John Wiley & Sons, Inc. Keywords: F cells r F reticulocytes r fetal hemoglobin r fetal red cells r fetomaternal hemorrhage r flow cytometry r hemoglobin levels r red blood cells How to cite this article: Davis, B. H. (2019). Enumeration of fetal red blood cells, hemoglobin-specific RBC cells, and F reticulocytes in human blood.

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Induction of fetal hemoglobin synthesis with recombinant human erythropoietin in anemic patients with heterozygous beta-thalassemia during pregnancy

Cited by 17 publications

References 36 publications

The Antioxidant Effect of Erythropoietin on Thalassemic Blood Cells

The Antioxidant Effect of Erythropoietin on Thalassemic Blood Cells

Use of erythropoietin in a pregnant Jehovah's Witness with sickle-cell disease

Enumeration of Fetal Red Blood Cells, Hemoglobin‐Specific RBC Cells, and F Reticulocytes in Human Blood

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