Induction of expression of inducible nitric oxide synthase by Taxol in murine macrophage cells

P02.22. Acupuncture is acceptable to children and adolescents undergoing cancer therapy

“…Nuclear protein complexes that bind specifically to NF-κB1 and NF-κB2 after treatment of RAW264.7 cells with C. crepidioides contained p50/p65/c-Rel. The components of the NF-κB complexes formed in response to LPS consisted primarily of p50, with very low levels of p65 and c-Rel subunits [18,21]. These results support the view that LPS and C. crepidioides extract activate NF-κB through the same signaling pathway.…”

“…C. crepidioides increased the expression of luciferase from the iNOS promoter in a dose-dependent manner (Figure 5A). The expression of the iNOS gene in macrophages is regulated mainly at the transcriptional level, particularly by NF-κB [18-21]. The murine iNOS promoter contains two putative NF-κB binding sites, one upstream (GGGATTTTCC, −971 to −962 bp, designated NF-κB2) and one downstream (GGGACTCTCC, −85 to −76 bp, designated NF-κB1).…”

“…Macrophage iNOS is not expressed in resting cells and differs from the constitutive neuronal and endothelial NOS [17]. Extensive studies of the transcriptional control of iNOS expression in murine macrophages have stressed the importance of binding of NF-κB [18-21], IRF-1 [27], Stat1 [28] and Oct-1 [29,30] to their recognition sequences on the iNOS promoter region for activation of iNOS transcription by interferon γ, LPS, IL-6 and Taxol. We showed here that C. crepidioides rapidly activated NF-κB.…”

Immunological effects of Taxol and Adryamicin in breast cancer patients

“…Other studies demonstrated that murine macrophages and human breast cancer cell lineages have higher expression of inducible nitric oxide synthase [11,12], nitric oxide synthesis [13][14][15], and reactive oxygen species generation [16][17][18]. Further, cytokines, as interleukin-1 beta (IL-1b), interleukin-6 (IL-6), and tumor necrosis factor (TNF-a) has been reported as significantly secreted products of macrophages during exposition to etoposide and 5-fluorouracil [19] and also described in breast cancer patients after anthracyclins-based regimens [20].…”

The role of Bcl-xL and nuclear factor-κB in the effect of taxol on the viability of dendritic cells

“…The effects of taxol on a variety of immune cells have been studied extensively. In taxol-treated macrophages, the expression levels of inducible nitric oxide synthase were elevated and the production of interleukin-12 (IL-12), which is a critical cytokine in innate and cell-mediated immunity, was increased [10,14]. Furthermore, it was suggested that taxol might enhance the cytotoxic activity of natural killer cells [12].…”