Induction of Expression of Genes Coding for Sporamin and β-Amylase by Polygalacturonic Acid in Leaf-Petiole Cuttings of Sweet Potato

Ohto, Masa‐aki; Nakamura-Kito, Kyoko; Nakamura, Kenzo

doi:10.1104/pp.99.2.422

Cited by 59 publications

(63 citation statements)

References 25 publications

(37 reference statements)

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“…Dissection of the promoter region of gSpoA1 using a b-glucuronidase (GUS) reporter in tobacco (Nicotiana tabacum) yielded a 210-bp sugar-inducible ''minimal'' promoter (Spo min ; Morikami et al, 2005). Expression of sporamin genes is also inducible by ABA (Ohto et al, 1992), and Spo min directed sugar-and ABA-inducible expression of GUS and luciferase (LUC) reporters in Arabidopsis. A transgenic Arabidopsis line harboring the Spo min T GUS-Spo min TLUC dual reporter genes was used to screen mutants displaying altered patterns of Sucinducible LUC and GUS expression, and a number of mutants that show higher levels of LUC and GUS expression under low-sugar conditions or lower levels of expression under high-sugar conditions compared to the wild type were isolated (A. Morikami, T. Saijo, M. Yamada, H. Tsukagoshi, T. Hattori, and K. Nakamura, unpublished data).…”

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confidence: 99%

Analysis of a Sugar Response Mutant of Arabidopsis Identified a Novel B3 Domain Protein That Functions as an Active Transcriptional Repressor

Tsukagoshi

Saijo²,

Shibata³

et al. 2005

Plant Physiology

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106

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A recessive mutation hsi2 of Arabidopsis (Arabidopsis thaliana) expressing luciferase (LUC) under control of a short promoter derived from a sweet potato (Ipomoea batatas) sporamin gene (Spo min TLUC) caused enhanced LUC expression under both lowand high-sugar conditions, which was not due to increased level of abscisic acid. The hsi2 mutant contained a nonsense mutation in a gene encoding a protein with B3 DNA-binding domain. HSI2 and two other Arabidopsis proteins appear to constitute a novel subfamily of B3 domain proteins distinct from ABI3, FUS3, and LEC2, which are transcription activators involved in seed development. The C-terminal part of HSI2 subfamily proteins contained a sequence similar to the ERFassociated amphiphilic repression (EAR) motif. Deletion of the C-terminal portion of HSI2 lost in the hsi2 mutant caused reduced nuclear targeting of HSI2. Null allele of HSI2 showed even higher Spo min TLUC expression than the hsi2 mutant, whereas overexpression of HSI2 reduced the LUC expression. Transient coexpression of 35STHSI2 with Spo min TLUC in protoplasts repressed the expression of LUC activity, and deletion or mutation of the EAR motif significantly reduced the repression activity of HSI2. These results indicate that HSI2 and related proteins are B3 domain-EAR motif active transcription repressors.In addition to transcriptional activators, transcriptional repressors play important roles in the regulation of transcription. Transcriptional repressors are basically classified into passive repressors and active repressors (Hanna-Rose and Hansen, 1996;Thiel et al., 2004). Passive repressors do not have intrinsic repressing activity and inhibit the activation of transcription by inhibiting the function of transcriptional activators through a competition with activators for binding with DNA or a formation of inactive heterodimers with activators. On the other hand, active repressors inhibit transcription in an activatorindependent manner by binding with basic transcription factors or corepressors.The expression of a number of plant genes is regulated by changes in sugar status via multiple signal transduction pathways (for review, see Koch, 1996;Smeekens, 2000;Rolland et al., 2002). Genetic analyses of sugar signaling mutants of Arabidopsis (Arabidopsis thaliana), obtained by genetic screen based on inhibition of germination and early seedling development by sugars, revealed a close link between sugar signaling and the production of ethylene and abscisic acid (ABA; for review, see Gazzarrini and McCourt, 2001;Rook and Bevan, 2003). In addition, direct screening based on sugar-responsive expression of endogenous genes or transgenes with reporters also resulted in identification of mutants with altered sugar-responsive gene expression (Dijkwel et al., 1997;Martin et al., 1997;Mita et al., 1997aMita et al., , 1997bRook et al., 2001; Baier et al., 2004). In general, these mutants affected the expression of a subset of sugar-regulated genes, and mutation either diminished or enhanced the sugarresponsive ex...

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confidence: 99%

Analysis of a Sugar Response Mutant of Arabidopsis Identified a Novel B3 Domain Protein That Functions as an Active Transcriptional Repressor

Tsukagoshi

Saijo²,

Shibata³

et al. 2005

Plant Physiology

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106

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“…The induced PIs represent either local [7,17] or systemic [18][19][20][21] responses, and either age-dependent [22] or organ-specific [8,18] traits. Application of chemical signals, such as jasmonic acid (JA), abscisic acid (ABA) and ethylene or salicylic acid (SA) can manipulate the production of PIs in plants [13,14,23,24], but the effects of each compound differ for different PIs.…”

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confidence: 99%

Salicylic acid and ethylene signaling pathways are involved in production of rice trypsin proteinase inhibitors induced by the leaf folder Cnaphalocrocis medinalis (Guenée)

Wang

Zhou

et al. 2011

Chin. Sci. Bull.

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The roles of signaling pathways in the production of trypsin proteinase inhibitors (TrypPIs) in rice infested by the leaf folder (LF) Cnaphalocrocis medinalis were studied. Infestation by LF increased TrypPI levels in the leaves of rice plants at the tillering, booting and flowering stages but decreased TrypPI levels at the ripening stage; TrypPI levels in rice stems did not increase at any developmental stage. Infestation by LF at the tillering stage systemically increased TrypPI levels in leaves but not in stems; it also enhanced salicylic acid (SA) levels in leaves and stems, and the ethylene level released from plants. However, LF infestation did not increase JA concentrations. Exogenous application of SA or ethylene enhanced TrypPI levels in the leaves and stems of plants at the tillering stage, whereas treatment with both SA and ethylene induced lower levels of TrypPIs than treatment with SA or ethylene alone, suggesting an antagonistic effect of SA and ethylene on TrypPIs induction. The results suggest that both SA and ethylene signaling pathways are involved in the production of TrypPIs in rice induced by LF; moreover, the antagonistic effect of SA and ethylene may explain the changes in TrypPI levels seen at different plant developmental stages and in different organs.rice, Cnaphalocrocis medinalis, jasmonic acid, salicylic acid, ethylene, trypsin proteinase inhibitor, herbivore-induced defense response Citation:Wang X, Hu L C, Zhou G X, et al. Salicylic acid and ethylene signaling pathways are involved in production of rice trypsin proteinase inhibitors induced by the leaf folder Cnaphalocrocis medinalis (Guenée).

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“…Amyβ expression in sweet potato (Yoshida et al 1992) is developmentally regulated in storage roots . However, Amyβ has been reported to be inducibly expressed in atypical tissues of sweet potato plants in response to high concentrations of sucrose, other metabolizable sugars, oligosaccharides, and ABA , Ohto et al 1992, Takeda et al 1995. We used sucrose induction to analyze Amyβ expression in leaf-petiole cuttings of non-sweet potato cultivars.…”

Section: Transcriptional Analysis Of the Amyβ-i Allelementioning

confidence: 99%

Nucleotide sequence variation associated with .BETA.-amylase deficiency in the sweet potato Ipomoea batatas (L.) Lam

et al. 2009

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Amyβ is a single gene that encodes β-amylase, a starch-hydrolyzing enzyme that confers sweetness to sweet potato roots upon cooking. To clarify the genetic basis underlying β-amylase null activity, we sequenced a highly conserved region of Amyβ, including the Amyβ active center, from normal and null cultivars. Comparison of the sequences revealed the presence of the In-Del sequences in null cultivars, which was thought to be a loss-of-function mutation causing a change from sweet to non-sweet. The In-Del frequency revealed using a multiple sequencing approach, and its association with β-amylase activity, showed that an inactive allele of Amyβ gene (Amyβ-I) exists. Amyβ expression analysis showed that Amyβ-I is transcribed normally, suggesting that translational control of β-amylase activity occurs in null mutants. The insertion in Amyβ-I caused a sequence frameshift resulting in an amino acid substitution with the incorporation of a stop codon in Amyβ-I. The Amyβ-I amino acid substitution and premature termination affect the substrate binding and catalytic site of the enzyme, which may result in an inactive protein that is responsible for the β-amylase inactivity in non-sweet cultivars. These findings are the basis of selection markers for non-sweetness in sweet potato breeding.

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Induction of Expression of Genes Coding for Sporamin and β-Amylase by Polygalacturonic Acid in Leaf-Petiole Cuttings of Sweet Potato

Abstract: Sporamin and fl-amylase are two major proteins of tuberous storage root of sweet potato (Ipomoea batatas) and their accumulation can be induced concomitantly with the accumulation of starch in leaves and petioles by sucrose (K Nakamura, M Ohto, N Yoshida, K Nakamura [1991]

Cited by 59 publications

References 25 publications

Analysis of a Sugar Response Mutant of Arabidopsis Identified a Novel B3 Domain Protein That Functions as an Active Transcriptional Repressor

Analysis of a Sugar Response Mutant of Arabidopsis Identified a Novel B3 Domain Protein That Functions as an Active Transcriptional Repressor

Salicylic acid and ethylene signaling pathways are involved in production of rice trypsin proteinase inhibitors induced by the leaf folder Cnaphalocrocis medinalis (Guenée)

Nucleotide sequence variation associated with .BETA.-amylase deficiency in the sweet potato Ipomoea batatas (L.) Lam

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