Induction of erythroblast increase in rabbit marrow cells by extracts of leukocytes and certain metabolic compounds. IV. Studies on erythropoiesis

Borsook, Henry; Jiggins, Susan; Wilson, Rosina T.

doi:10.1002/jcp.1040790212

Cited by 7 publications

(2 citation statements)

References 16 publications

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“…Some studies have demonstrated that cAMP stimulates in vitro erythropoiesis as measured by such parameters as heme synthesis or erythroblast numbers (24)(25)(26)(27) although others have not shown such an effect (28,29). In fetal rat liver, although stimulation of heme synthesis by ESF has been shown, no effect of ESF on cAMP levels was noted.…”

Section: Discussionmentioning

confidence: 99%

Modulation of in vitro erythropoiesis. The influence of beta-adrenergic agonists on erythroid colony formation.

Brown¹,

Adamson²

1977

J. Clin. Invest.

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Section: Discussionmentioning

confidence: 99%

Modulation of in vitro erythropoiesis. The influence of beta-adrenergic agonists on erythroid colony formation.

Brown¹,

Adamson²

1977

J. Clin. Invest.

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“…Bottomley ct a/ (1971) reported that cAMP and theophylline increased A-aminolevulinic acid synthetase activity of rabbit marrow cells but observed no illcrease in haem synthesis. Several investigators have described stimulation of pronormoblast proliferation by cAMP (Borsook et al, 1972;Tisman & Herbert, 1973).…”

Section: Discussionmentioning

confidence: 99%

Studies of the Influence of Cyclic Nucleotides on in Vitro Haemoglobin Synthesis

Brown

Adamson

1977

Br J Haematol

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The influence of various cyclic nucleotides on in vitro haemoglobin synthesis has been examined in suspension cultures of mammalian marrow cells. Over a wide range of concentrations, dibutyryl cyclic AMP (db-cAMP) was either ineffective or inhibited haemoglobin synthesis by marrow cells from rat, mouse and guinea-pig. However, 10(-3) M db-cAMP consistently stimulated haemoglobin synthesis in cultures of human, sheep, rabbit and canine cells, with the latter being most responsive. This effect, which approached in magnitude that of erythropoietin (ESF) itself, was specific for cAMP and its mono- and dibutyryl derivatives and was not inhibited by anti-ESF. Adenosine, AMP, ADP, ATP, cGMP, db-cGMP, cCMP, cIMP and sodium butyrate were either inactive or inhibitory at similar concentrations. Enhancement of haemoglobin synthesis was also observed with the phosphodiesterase inhibitor, RO-20-1724. The susceptibility to ionizing radiation of the response to ESF and db-cAMP was marked, indicating that the increased haemoglobin synthesis in this system was proliferation dependent, although the response to db-cAMP was less radiosensitive. Studies with tritiated thymidine showed that about 50% of the cells which were responding to either db-cAMP or ESF were actively engaged in DNA synthesis. However, the physical characteristics of db-cAMP-and ESF-responsive cells were dissimilar as analysed by their velocity sedimentation properties. These studies demonstrate that cAMP has a major stimulatory effect on haemoglobin synthesis with cells from selected mammalian species with activity approaching that of ESF, but the target cells most responsive to these agents appear different. The results suggest that cyclic nucleotide-related mechanisms may modulate in vitro erythropoiesis.

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Stimulation of rabbit erythroblast multiplication and maturation in vitro by blood leukocytes: Cytological observations. III. Studies on erythropoiesis

Borsook

Jiggins

Wilson

1972

Journal Cellular Physiology

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Blood leukocytes incubated in vitro with rabbit-marrow cells induced a several-fold increase in basophilic erythroblasts and smaller increases in acidophils and reticulocytes. The main effect was nearly complete in one hour at 37". Erythropoietin augmented the leukocyte effect; anti-erythropoietin inhibited i t with or without erythropoietin. The erythroblast increase came entirely from the marrow cells; the precursor cell class has not been identified, except that division of pre-existing basophils appears to be excluded. Autologous and homologous leukocytes were about equally effective.A method is described of measuring on stained smears changes in relative concentrations of different classes of cells induced experimentally. A method of preparing highly concentrated peripheral blood leukocytes is described.

show abstract

Induction of erythroblast increase in rabbit marrow cells by extracts of leukocytes and certain metabolic compounds. IV. Studies on erythropoiesis

Cited by 7 publications

References 16 publications

Modulation of in vitro erythropoiesis. The influence of beta-adrenergic agonists on erythroid colony formation.

Modulation of in vitro erythropoiesis. The influence of beta-adrenergic agonists on erythroid colony formation.

Studies of the Influence of Cyclic Nucleotides on in Vitro Haemoglobin Synthesis

Stimulation of rabbit erythroblast multiplication and maturation in vitro by blood leukocytes: Cytological observations. III. Studies on erythropoiesis

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