Induction of endolymphatic hydrops in the guinea pig by perisaccular deposition of sepharose beads carrying and not carrying immune complexes

Time sequence of degeneration pattern in the guinea pig cochlea during cisplatin administration.

“…Endolymphatic hydrops is the net result of an imbalance between endolymph production and resorption, which are generally thought to take place in the stria vascularis and the endolymphatic sac, respectively. In this study, we again have used the l R /d ratio as an objective measure of the degree of endolymphatic hydrops (cf., Bouman et al, 1998). The l R /d values obtained in the experimental groups did not differ significantly from those obtained in the non-treated group, not even in the 8-day group.…”

“…6. In all groups, higher l R /d values for the lower basal turn (b1) were observed, but this is a common finding using our fixation protocol (cf., Bouman et al, 1998;Cardinaal et al, 2000a). No significant changes in l R /d values were observed between the non-treated group and the experimental groups, implying that cisplatin administration did not result in a distension of ReissnerÕs membrane (i.e., endolymphatic hydrops).…”

“…In order to quantify the degree of distension of ReissnerÕs membrane-which may be regarded as a histological indication of strial dysfunction-the ratio l R /d was used as an objective measure of endolymphatic hydrops (Bouman et al, 1998). This ratio is calculated by dividing the actual length of ReissnerÕs membrane, l R , by the rectilinear distance, d, between its medial (i.e., the spiral limbus) and its lateral (i.e., the stria vascularis) attachment points.…”

Interferon‐γ suppresses Na+–H+ exchanger in cultured human endolymphatic sac epithelial cells

“…The pathogenesis of the endolymphatic hydrops underlying Meniere's disease is still unclear, although an autoimmune basis has been suggested [Bouman et al, 1998]. The Th1 subset of lymphocytes, in particular, seems to be increased in Meniere's disease [Fuse et al, 2003], and IFN‐γ production by Th1 cells is elevated in animal models [Tomiyama, 2001; Pawankar et al, 2004].…”

A Pilot Study of Rituximab in Immune-Mediated Inner Ear Disease

“…Bouman et al [1998] demonstrated, using a guinea pig model, that immunization with swine inner ear antigens in complete Freund's adjuvant induced cochlear dysfunction, endolymphatic hydrops and presence of antibodies to 68-, 50-, 45-and 27-kDa molecular weight inner ear antigens [Nair et al, 2004]. It has been reported that the target antigen that is putatively involved in IMED may be a choline transporter-like protein 2, which is an inner ear glycoprotein of 68 and 72 kDa [Nair et al, 2004].…”