Induction of dopamine D1 and D5 receptors in R28 cells by light exposures

Ke, Yan; Li, Wentao; Tan, Zhiqun; Yang, Zhikuan

doi:10.1016/j.bbrc.2017.03.099

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…Notably, Drd4 was among the top downregulated genes in highly myopic ACs (Figure 4H). As a component of D2‐like dopamine receptors (D2Rs), Drd4 could actively respond to the environmental lighting by modulating intracellular Ca 2+ levels and therefore regulating Ca 2+ ‐dependent signaling processes 21 . This result supports previous observations suggesting that D2Rs, rather than D1‐like receptors (D1Rs), mediate the effect of dopamine for protecting against lens‐induced myopia 22,23 .…”

Section: Resultssupporting

confidence: 86%

“…As a component of D2-like dopamine receptors (D2Rs), Drd4 could actively respond to the environmental lighting by modulating intracellular Ca 2+ levels and therefore regulating Ca 2+ -dependent signaling processes. 21 This result supports previous observations suggesting that D2Rs, rather than D1-like receptors (D1Rs), mediate the effect of dopamine for protecting against lens-induced myopia. 22,23 In summary, these findings strongly suggest that abnormal BC/AC-mediated light processing within the retina may be the vital factor that triggers and maintains myopia development.…”

Section: Decreased Neuronal Excitability Of Interneurons and Imbalanc...supporting

confidence: 91%

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Single‐cell RNA sequencing of retina revealed novel transcriptional landscape in high myopia and underlying cell‐type‐specific mechanisms

Yao,

Chen,

et al. 2023

MedComm

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High myopia is a leading cause of blindness worldwide with increasing prevalence. Retina percepts visual information and triggers myopia development, but the underlying etiology is not fully understood because of cellular heterogeneity. In this study, single‐cell RNA sequencing analysis was performed on retinas of mouse highly myopic and control eyes to dissect the involvement of each cell type during high myopia progression. For highly myopic photoreceptors, Hk2 inhibition underlying metabolic remodeling from aerobic glycolysis toward oxidative phosphorylation and excessive oxidative stress was identified. Importantly, a novel Apoe+ rod subpopulation was specifically identified in highly myopic retina. In retinal neurons of highly myopic eyes, neurodegeneration was generally discovered, and the imbalanced ON/OFF signaling driven by cone‐bipolar cells and the downregulated dopamine receptors in amacrine cells were among the most predominant findings, indicating the aberrant light processing in highly myopic eyes. Besides, microglia exhibited elevated expression of cytokines and TGF‐β receptors, suggesting enhanced responses to inflammation and the growth‐promoting states involved in high myopia progression. Furthermore, cell–cell communication network revealed attenuated neuronal interactions and increased glial/vascular interactions in highly myopic retinas. In conclusion, this study outlines the transcriptional landscape of highly myopic retina, providing novel insights into high myopia development and prevention.

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Section: Resultssupporting

confidence: 86%

Section: Decreased Neuronal Excitability Of Interneurons and Imbalanc...supporting

confidence: 91%

Single‐cell RNA sequencing of retina revealed novel transcriptional landscape in high myopia and underlying cell‐type‐specific mechanisms

Yao,

Chen,

et al. 2023

MedComm

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“…The retinal progenitor cell line R28 was established in the late 1990s starting from a 6-day-old immortalized rat retinal culture. R28 cells express the glial cell markers GFAP, S-100, and vimentin and respond to neurotransmitters and light stimulation, suggesting the presence of different retinal neurotransmitter receptors [18,19,24,25]. After cultivation of the cell lines on glass, we determined a higher number of dead cells than on the flexible polyimide bases.…”

Section: Discussionmentioning

confidence: 95%

New epiretinal implant with integrated sensor chips for optical capturing shows a good biocompatibility profile in vitro and in vivo

et al. 2021

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Background Retinal degenerative diseases, e.g., retinitis pigmentosa, cause a severe decline of the visual function up to blindness. Treatment still remains difficult; however, implantation of retinal prostheses can help restoring vision. In this study, the biocompatibility and surgical feasibility of a newly developed epiretinal stimulator (OPTO-EPIRET) was investigated. The previously developed implant was extended by an integrated circuit-based optical capturing, which will enable the immediate conversion of the visual field into stimulation patterns to stimulate retinal ganglion cells. Results The biocompatibility of the OPTO-EPIRET was investigated in vitro using the two different cell lines L-929 and R28. Direct and indirect contact were analyzed in terms of cell proliferation, cell viability, and gene expression. The surgical feasibility was initially tested by implanting the OPTO-EPIRET in cadaveric rabbit eyes. Afterwards, inactive devices were implanted in six rabbits for feasibility and biocompatibility testings in vivo. In follow-up controls (1–12 weeks post-surgery), the eyes were examined using fundoscopy and optical coherence tomography. After finalization, histological examination was performed to analyze the retinal structure. Regarding the in vitro biocompatibility, no significant influence on cell viability was detected (L929: < 1.3% dead cells; R-28: < 0.8% dead cells). The surgery, which comprised phacoemulsification, vitrectomy, and implantation of the OPTO-EPIRET through a 9–10 mm corneal incision, was successfully established. The implant was fixated with a retinal tack. Vitreal hemorrhage or retinal tearing occurred as main adverse effects. Transitional corneal edema caused difficulties in post-surgical imaging. Conclusions The OPTO-EPIRET stimulator showed a good biocompatibility profile in vitro. Furthermore, the implantation surgery was shown to be feasible. However, further design optimization steps are necessary to avoid intra- and postoperative complications. Overall, the OPTO-EPIRET will allow for a wide visual field and good visual acuity due to a high density of electrodes in the central retina.

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Research Progress on Etiology and Pathogenesis of Myopia

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2020

ACM

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Induction of dopamine D1 and D5 receptors in R28 cells by light exposures

Cited by 3 publications

References 31 publications

Single‐cell RNA sequencing of retina revealed novel transcriptional landscape in high myopia and underlying cell‐type‐specific mechanisms

Single‐cell RNA sequencing of retina revealed novel transcriptional landscape in high myopia and underlying cell‐type‐specific mechanisms

New epiretinal implant with integrated sensor chips for optical capturing shows a good biocompatibility profile in vitro and in vivo

Research Progress on Etiology and Pathogenesis of Myopia

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