Induction of DNA Strand Scissions in HeLa Cells by Human Polymorphonuclear Leucocytes Activated by Chlamydia trachomatis Elementary Bodies

Zvillich, Menachem; Kol, R.; Riklis, E.; Sarov, Israel

doi:10.1099/00221287-134-8-2405

Cited by 4 publications

(2 citation statements)

References 30 publications

(30 reference statements)

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“…Data on release of toxic oxygen-derived molecules are conflicting. It has been reported that C. trachomatis biovars trachoma and LGV inhibit the respiratory burst in PMNs at the level of NADPH oxidase (469) and that they do not cause an increase in superoxide levels in PMNs 477, but in another study (539), it was found that the LGV biovar stimulates the production of superoxide anions and hydrogen peroxide by PMNs. PMNs from individuals deficient in myeloperoxidase inactivate chlamydiae normally, indicating that other mechanisms are also important (527).…”

Section: Destruction Of Chlamydiaementioning

confidence: 93%

Interaction of chlamydiae and host cells in vitro.

Moulder¹

1991

Microbiological Reviews

513

288

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Section: Destruction Of Chlamydiaementioning

confidence: 93%

Interaction of chlamydiae and host cells in vitro.

Moulder¹

1991

Microbiological Reviews

513

288

View full text Add to dashboard Cite

“…5). Superoxide anions (-Oy) and hydrogen peroxide (H202) are also produced by EB-activated HPMN (Zvillich & Sarov, 1988). Several Gram-negative bacteria, as well as lipopolysaccharide and the synthetic N-formylated methionyl oligopeptide analogous to the NH2-terminal degradation product of bacterial proteins, can induce the production of H202, 'OZand CL by HPMN (Bradley, 1979;Dahlgren & Stendahl, 1982;Robinson et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

The Persistence of Chlamydia trachomatis Elementary Body Cell Walls in Human Polymorphonuclear Leucocytes and Induction of a Chemiluminescent Response

Zvillich

Sarov

1989

Microbiology

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Human polymorphonuclear leucocytes (HPMN) were incubated with [35S]methionine-labelled Chlamydia trachomatis (serovar L2/434/Bu) elementary bodies (EB) and EB cell walls. No net loss in the TCA-precipitable radioactivity was observed over 24 h in the HPMN that had taken up EB cell walls. SDS-polyacrylamide gel electrophoresis of the labelled C. trachomatis EB and EB cell wall proteins extracted from the HPMN at 2 and 24 h after infection demonstrated the persistence of most of the chlamydial cell wall polypeptides. Analysis of extracts of the HPMN that had taken up either EB or EB cell walls on Urografin density gradients at 2 and 24 h after infection, and electron microscopic observations on fractions representing the peaks, demonstrated the persistence of the EB cell walls in the HPMN. Electron microscopic observations of HPMN that had taken up EB or EB cell walls demonstrated EB cell walls in the HPMN phagosomes at 24 h after infection. The HPMN exposed to EB and EB cell walls of C. trachomatis gave chemiluminescent (CL) responses with peaks respectively 12 and 7 times greater than the peak value of the control. The significance of the persistence of the EB cell wall polypeptides and cell walls in the HPMN and activation of the HPMN to produce oxygen radicals (i.e. a CL response), and its possible relation to rheumatic diseases, is discussed.

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Implications for persistent chlamydial infections of phagocyte-microorganism interplay

Sarov

Geron

Shemer‐Avni

et al. 1991

Eur. J. Clin. Microbiol. Infect. Dis.

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In vitro models of Chlamydia trachomatis inhibition by cytokines, human-monocyte derived macrophages (HMDM) and human polymorphonuclear leukocytes (HPMN) are discussed in an attempt to delineate the molecular basis of parasite-host cell interplay in persistent and chronic chlamydial infection. Interferon gamma (IFN) has been found to reversibly inhibit chlamydial growth at an early stage in the replicative cycle, while tumor necrosis factor (TNF) has a more profound effect on chlamydial growth resulting in production of aberrant reticulate bodies and enhancement of production of prostaglandin E2 (PGE2). Chlamydia trachomatis (serovar L2) replicate in HMDM while serovar K has been found to be restricted in these cells. Chlamydiae are killed by HPMN but the cell walls persist undegraded, inducing production of oxygen radicals which can be demonstrated to induce DNA strand scissions in HeLa target cells. Evidence is accumulating that chlamydia specific serum IgA antibodies may serve as a noninvasive serological marker for diagnosis of a number of acute and persistent Chlamydia trachomatis infections.

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Induction of DNA Strand Scissions in HeLa Cells by Human Polymorphonuclear Leucocytes Activated by Chlamydia trachomatis Elementary Bodies

Cited by 4 publications

References 30 publications

Interaction of chlamydiae and host cells in vitro.

Interaction of chlamydiae and host cells in vitro.

The Persistence of Chlamydia trachomatis Elementary Body Cell Walls in Human Polymorphonuclear Leucocytes and Induction of a Chemiluminescent Response

Implications for persistent chlamydial infections of phagocyte-microorganism interplay

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