Induction of DNA Double-strand Breaks in CHO-K1 Cells by Carbon Ions

Heilmann, J.; Taucher-Scholz, G.; Kraft, Gerhard

doi:10.1080/09553009514551051

Cited by 43 publications

(18 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The survival curves for carbon ion irradiation are almost linear on the semi-logarithmic scale. The D 10 value indicates the dose required to reduce the surviving fraction to 10%; the D 10 doses for the Sq. C.C., FS and HP cells after γ-ray irradiation were 5.7, 4.6 and 3.7 Gy, respectively, showing a broad range of radiosensitivity.…”

Section: Resultsmentioning

confidence: 99%

“…The higher the LET, the greater the chance of inducing multiple sites of DNA damage and complex lesions. At such sites, small fragments can be produced [10] and because these are lost due to cell lysis as LET increases, the number of measured DNA strand breaks is underestimated with high LET radiation [27]. Currently, there are no techniques that can detect cluster damage precisely, and most current techniques detect cluster damage as a single DSB [1].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prediction of Cellular Radiosensitivity from DNA Damage Induced by .GAMMA.-Rays and Carbon Ion Irradiation in Canine Tumor Cells

Wada¹,

Khoa²,

Kobayashi³

et al. 2005

J. Vet. Med. Sci.

View full text Add to dashboard Cite

ABSTRACT. Diseases of companion animals are shifting from infectious diseases to neoplasms (cancer), and since radiation therapy is one of the effective choices available for cancer treatment, the application of radiotherapy in veterinary medicine is likely to increase. However tumor tissues have different radiosensitivities, and therefore it is important to determine the intrinsic radiosensitivity of tumors in individual patients in advance of radiotherapy. We have studied the relationship between the surviving cell fraction measured by a clonogenic assay and DNA double strand breaks detected by a comet assay under neutral conditions in three canine tumor cell lines, after γ-ray and carbon ion irradiation. In all the cell lines, cell death assessed by the clonogenic assay was much higher following irradiation with carbon ions than with γ-rays. The initial and residual (4 hr) DNA damage due to γ-ray and carbon ion irradiation were higher in a radiosensitive cell line than in a radioresistant cell line. The surviving cell fraction at 2 Gy (SF2) showed a tendency for correlation with both the initial and residual DNA damage. In particular, the residual damage per Gy was significantly correlated with SF2, regardless of the type of radiation. This indicates that cellular radiosensitivity can be predicted by detection of radiation-induced residual DNA damage. KEY WORDS: canine tumor cell, comet assay, DNA damage, radiosensitivity, radiotherapy.J. Vet. Med. Sci. 67(11): 1089-1095, 2005 Due to recent improvements in the breeding environment and veterinary preventive medicine, companion animals live much longer than in the past. These improvements have made it possible to reduce severe infectious diseases, but conversely the incidence of tumors is increasing in animals. Radiation therapy is one of the effective choices available against such tumors and its use in veterinary medicine is likely to increase.In human medicine, advances have been made in new technologies, such as low linear energy transfer (LET) radiation using protons [16] and linear accelerator (LINAC) three dimensional X-rays. Because high LET radiation causes higher reproductive cell death than proton irradiation [5], clinical studies of radiation therapy using heavy-ion beams have started. However, a wide range of radiosensitivity is observed, even for tumors of the same histopathological origin [2]. Therefore, to improve local control of the tumor, and to set up an effective treatment plan with minimal side effects, it is necessary to predict the radiosensitivity of the tumor for an individual patient, both for high and low LET radiation. Therefore, development of a predictive assay for tumor radiosensitivity in medical practice and establishment of biological parameters are of importance.In general, the clonogenic survival assay is regarded as a good measure of radiosensitivity related to the effects of radiotherapy [4]. A good correlation has been found between the reproductive death of fibroblasts measured by the clonogenic assay and the severity of...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Prediction of Cellular Radiosensitivity from DNA Damage Induced by .GAMMA.-Rays and Carbon Ion Irradiation in Canine Tumor Cells

Wada¹,

Khoa²,

Kobayashi³

et al. 2005

J. Vet. Med. Sci.

View full text Add to dashboard Cite

show abstract

“…Conventional low linear energy transfer (LET) radiation produces approximately 30 DSB per Gy in Chinese hamster ovary (CHO) cells (12,14). Karnas et al (12) measured an increased yield due to Auger electrons of 3.4 DSB per cell per Gy of dose to water using tungsten-filtered 100-kVp xrays in CHO cells with 18% thymidine replacement by IUdR.…”

Section: Introductionmentioning

confidence: 99%

Dependence of Cell Survival on Iododeoxyuridine Concentration in 35-keV Photon-Activated Auger Electron Radiotherapy

Dugas

Varnes

Sajo

et al. 2011

International Journal of Radiation Oncology*Biology*Physics

View full text Add to dashboard Cite

“…Unfortunately, the calculation has been performed in the LET approximation only, neglecting any influence of track structure on the measured DSB yields. In addition the comparison between theory and data is somewhat disfavoured by the quality of the reference data, which could not be confirmed by subsequent measurements [31].…”

Section: Other Approachesmentioning

confidence: 98%

A track structure model for simulation of strand breaks in plasmid DNA after heavy ion irradiation

Brons

Taucher-Scholz

Scholz

et al. 2003

Radiat Environ Biophys

Self Cite

View full text Add to dashboard Cite

We present a track structure model based on the local dose deposited around heavy ion tracks to explain the cross sections for single-strand and double-strand break induction in plasmid DNA in different aqueous buffers. The model is based only on measurable quantities, namely the effect distribution for inducing strand breaks after x-ray irradiation as a function of dose, and the radial dose distribution of the heavy ion track. The effect of indirect DNA damage mediated by free radicals produced in the water surrounding the DNA is accounted for by allowing the radial dose distribution to be smeared in space by an effective target size corresponding to the squared sum of the geometrical extension of the plasmid molecule and the mean free drift path of the radicals in the buffer solution. Our calculations reproduce well the measured cross sections for single-strand and double-strand break induction in SV40 plasmid DNA in various buffer solutions both as a function of the LET and of the specific energy of the heavy ion.

show abstract

Induction of DNA Double-strand Breaks in CHO-K1 Cells by Carbon Ions

Cited by 43 publications

References 41 publications

Prediction of Cellular Radiosensitivity from DNA Damage Induced by .GAMMA.-Rays and Carbon Ion Irradiation in Canine Tumor Cells

Prediction of Cellular Radiosensitivity from DNA Damage Induced by .GAMMA.-Rays and Carbon Ion Irradiation in Canine Tumor Cells

Dependence of Cell Survival on Iododeoxyuridine Concentration in 35-keV Photon-Activated Auger Electron Radiotherapy

A track structure model for simulation of strand breaks in plasmid DNA after heavy ion irradiation

Contact Info

Product

Resources

About