Induction of DNA damage by low level laser irradiation in Friend mouse erythroleukaemia cells

McKelvey, V.J.; Keegan, A.L.; Allen, Joshua A. M.

doi:10.1016/0165-1161(92)91121-7

Cited by 6 publications

(2 citation statements)

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“…Therefore, the doses used in our study may result in induction of DNA damage below the detection limit of the comet assay. This possibility is supported by the fact that at higher doses used by other investigators, induction of DNA damage [29] and impairment of cell function has indeed been reported [30,31].…”

Section: Discussionmentioning

confidence: 57%

He-Ne laser irradiation protects B-lymphoblasts from UVA-induced DNA damage

Dube¹,

Bock²,

Bauer³

et al. 2001

Radiation and Environmental Biophysics

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The effect of He-Ne laser (632.8 nm) pre-irradiation on UVA (343 nm)-induced DNA damage in the human B-lymphoblast cell line NC37 was investigated using the comet assay. He-Ne laser pre-irradiation was observed to result in a dose-dependent decrease in UVA-induced DNA damage. This effect was also found to be dependent on the incubation period between He-Ne laser pre-irradiation and the UVA exposure. Whereas the control cells with a higher DNA damage point to an initial ability of faster repair, both the control and the He-Ne laser pre-irradiated cells subsequently show the same rate of DNA repair. The results suggest that He-Ne laser irradiation protect the cells from UVA-induced DNA damage primarily through an influence on processes that prevent an initial DNA damage.

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Section: Discussionmentioning

confidence: 57%

He-Ne laser irradiation protects B-lymphoblasts from UVA-induced DNA damage

Dube¹,

Bock²,

Bauer³

et al. 2001

Radiation and Environmental Biophysics

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“…High fluence LPLI-induced DNA damage has been detected using the single cell Comet assay [33]. Caspase family is nearly connected with many apoptotic processes.…”

Section: Discussionmentioning

confidence: 99%

Measuring dynamics of caspase-3 activity in living cells using FRET technique during apoptosis induced by high fluence low-power laser irradiation

et al. 2005

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Low intensity laser irradiation inhibits tritiated thymidine incorporation in the hemopoietic cell lines HL‐60 and U937

et al. 1994

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The purpose of this study was to determine the effect of low intensity laser irradiation (660 nm, 12 mW, 5 kHz) on tritiated thymidine incorporation in two hemopoietic cell lines, HL-60 and U937. Cells were suspended at a concentration of 1 x 10(6)/ml in their respective serum-free media and irradiated at energy densities from 1.0 to 11.5 J/cm2. Twenty-four hours after irradiation the cells were assayed for their ability to incorporate tritiated thymidine (3H-TdR) in comparison with nonirradiated cells. Analysis by two-way analysis of variance (ANOVA) for unrelated groups showed that laser irradiation at all energy densities > or = 5.8 J/cm2 produced a significant decrease in 3H-TdR incorporation (P < 0.05) into HL-60 cells. In U937 cells, irradiation at energy densities of 5.8, 7.2, and 11.5 J/cm2 caused a similar reduction in 3H-TdR incorporation (P < 0.01), although not at 8.6 and 9.6 J/cm2. The temperature of each cell suspension was recorded both during and immediately postirradiation, and no significant thermal changes were observed. These findings demonstrate a direct photobiological effect of laser irradiation on these two cell lines. The precise mechanism for this effect is unknown but may have significance in understanding the biological action of laser's known therapeutic effectiveness in promoting wound repair.

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Induction of DNA damage by low level laser irradiation in Friend mouse erythroleukaemia cells

Cited by 6 publications

References 0 publications

He-Ne laser irradiation protects B-lymphoblasts from UVA-induced DNA damage

He-Ne laser irradiation protects B-lymphoblasts from UVA-induced DNA damage

Measuring dynamics of caspase-3 activity in living cells using FRET technique during apoptosis induced by high fluence low-power laser irradiation

Low intensity laser irradiation inhibits tritiated thymidine incorporation in the hemopoietic cell lines HL‐60 and U937

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