Induction of disease resistance in common bean susceptible to halo blight bacterial pathogen after seed bacterization with rhizosphere pseudomonads.

Alström, Sadhna

doi:10.2323/jgam.37.495

Cited by 174 publications

(71 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This strain was shown to be a more aggressive pathogen than the strain P. syringae pv. phaseolicola, 52 used earlier in our laboratory (2). The strain N7 6ar was grown on King's medium B agar (9) from its lyophilized cultures and suspended in 0.01 M MgSO4 solution when used.…”

Section: Methodsmentioning

confidence: 99%

“…Seed bacterization with this strain was in an earlier study demonstrated to induce disease resistance in Phaseolus vulgaris against the pathogen, Pseudomonas syringae pv. phaseolicola strain 52 (2). In this paper further results are presented on the ability of this strain to induce systemic resistance against the halo blight pathogen.…”

mentioning

confidence: 99%

“…Induced resistance is associated with increased synthesis of certain enzymes such as peroxidase (11), increased levels of certain acid-soluble proteins (20) and accumulation of phytoalexins in the induced plant tissue (16). Pseudomonas fluorescens strain S97 is a PGPR and an antagonist of both fungal and bacterial pathogens (1,2). It is known to produce various metabolites, one of which is a phytotoxin and identified as hydrogen cyanide (1,3 ).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Evidence of disease resistance induced by rhizosphere pseudomonad againstPseudomonas syringae pv. phaseolicola.

Alström

1995

J. Gen. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Evidence of disease resistance induced by rhizosphere pseudomonad againstPseudomonas syringae pv. phaseolicola.

Alström

1995

J. Gen. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

“…This phenomenon, termed induced systemic resistance (ISR), has been demonstrated for various rhizobacteria in several plants (2,19,24,36,40). The induced resistance reduces disease symptoms of a wide range of pathogens (10,(20)(21)(22)40), and its physiological characterization is in progress.…”

mentioning

confidence: 99%

Salicylic Acid Produced by the Rhizobacterium Pseudomonas aeruginosa 7NSK2 Induces Resistance to Leaf Infection by Botrytis cinerea on Bean

Meyer¹,

Höfte²

1997

Phytopathology®

285

145

View full text Add to dashboard Cite

De Meyer, G., and Höfte, M. 1997. Salicylic acid produced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 induces resistance to leaf infection by Botrytis cinerea on bean. Phytopathology 87:588-593.Selected strains of nonpathogenic rhizobacteria can induce a systemic resistance in plants that is effective against various pathogens. In an assay with bean plants, we investigated which determinants of the rhizobacterium Pseudomonas aeruginosa 7NSK2 are important for induction of resistance to Botrytis cinerea. By varying the iron nutritional state of the bacterium at inoculation, it was demonstrated that induced resistance by P. aeruginosa 7NSK2 was iron-regulated. As P. aeruginosa 7NSK2 produces three siderophores under iron limitation, pyoverdin, pyochelin, and salicylic acid, we investigated the involvement of these iron-regulated metabolites in induced resistance by using mutants deficient in one or more siderophores. Results demonstrated that salicylic acid production was essential for induction of resistance to B. cinerea by P. aeruginosa 7NSK2 in bean and did not exclude a role for pyochelin. A role for pyoverdin, however, could not be demonstrated. Transcriptional activity of salicylic acid and pyochelin biosynthetic genes was detected during P. aeruginosa 7NSK2 colonization of bean. Moreover, the iron nutritional state at inoculation influenced the transcriptional activity of salicylic acid and pyochelin biosynthetic genes in the same way as it influenced induction of systemic resistance to B. cinerea.

show abstract

“…tomato pathogenicity (20), including the sources of genetic resistance to the pathogen (6,18,33,34,39,50; V. F. Lawson and W. L. Summers, abstr., HortScience 17:503) and the genes which confer resistance to the disease (30, 41), this knowledge has not yet translated into an efficient strategy to control this minor, but occasionally devastating, foliar disease. Disease control is still based on traditional chemical and physical methods (3), and despite some significant successes, achieved mainly by the induction of systemic resistance in plants (1,27,45,52) and the displacement of a pathogen by nonvirulent strains of the same pathogen or by ecologically similar antagonistic strains (28,43,44,48,49), biological control of foliar bacterial pathogens is still largely at the experimental stage.The aim of this study was to measure the fluctuations in the populations of the two bacterial species, belonging to different genera, on the foliage and in the rhizospheres of tomato plants inoculated with one or both species. The effect of the relative sizes of the bacterial populations on the development of bacterial leaf speck disease in tomato plants and on plant growth was monitored.…”

mentioning

confidence: 99%

Protection of Tomato Seedlings against Infection by Pseudomonas syringae pv. Tomato by Using the Plant Growth-Promoting Bacterium Azospirillum brasilense

Bashan¹,

de‐Bashan

2002

Appl Environ Microbiol

120

View full text Add to dashboard Cite

Pseudomonas syringae pv. tomato, the causal agent of bacterial speck of tomato, and the plant growthpromoting bacterium Azospirillum brasilense were inoculated onto tomato plants, either alone, as a mixed culture, or consecutively. The population dynamics in the rhizosphere and foliage, the development of bacterial speck disease, and their effects on plant growth were monitored. When inoculated onto separate plants, the A. brasilense population in the rhizosphere of tomato plants was 2 orders of magnitude greater than the population of P. syringae pv. tomato (10 7 versus 10 5 CFU/g [dry weight] of root). Under mist chamber conditions, the leaf population of P. syringae pv. tomato was 1 order of magnitude greater than that of A. brasilense (10 7 versus 10 6 CFU/g [dry weight] of leaf). Inoculation of seeds with a mixed culture of the two bacterial strains resulted in a reduction of the pathogen population in the rhizosphere, an increase in the A. brasilense population, the prevention of bacterial speck disease development, and improved plant growth. Inoculation of leaves with the mixed bacterial culture under mist conditions significantly reduced the P. syringae pv. tomato population and significantly decreased disease severity. Challenge with P. syringae pv. tomato after A. brasilense was established in the leaves further reduced both the population of P. syringae pv. tomato and disease severity and significantly enhanced plant development. Both bacteria maintained a large population in the rhizosphere for 45 days when each was inoculated separately onto tomato seeds (10 5 to 10 6 CFU/g [dry weight] of root). However, P. syringae pv. tomato did not survive in the rhizosphere in the presence of A. brasilense. Foliar inoculation of A. brasilense after P. syringae pv. tomato was established on the leaves did not alleviate bacterial speck disease, and A. brasilense did not survive well in the phyllosphere under these conditions, even in a mist chamber. Several applications of a low concentration of buffered malic acid significantly enhanced the leaf population of A. brasilense (>10 8 CFU/g [dry weight] of leaf), decreased the population of P. syringae pv. tomato to almost undetectable levels, almost eliminated disease development, and improved plant growth to the level of uninoculated healthy control plants. Based on our results, we propose that A. brasilense be used in prevention programs to combat the foliar bacterial speck disease caused by P. syringae pv. tomato.Tomato plants are hosts to Azospirillum brasilense, a plant growth-promoting bacterium (PGPB) (7,8), and Pseudomonas syringae pv. tomato, the causal agent of bacterial speck disease (35,47). This disease is of moderate economic importance to tomato production under greenhouse and field conditions (24). Whereas A. brasilense increases the growth and yield of tomato plants (14), P. syringae pv. tomato decreases production (51). Although A. brasilense is known as a rhizosphere bacterium (7), some strains are ephiphytic (7, 10), and P. syringae pv. tomato is...

show abstract

Induction of disease resistance in common bean susceptible to halo blight bacterial pathogen after seed bacterization with rhizosphere pseudomonads.

Cited by 174 publications

References 7 publications

Evidence of disease resistance induced by rhizosphere pseudomonad againstPseudomonas syringae pv. phaseolicola.

Evidence of disease resistance induced by rhizosphere pseudomonad againstPseudomonas syringae pv. phaseolicola.

Salicylic Acid Produced by the Rhizobacterium Pseudomonas aeruginosa 7NSK2 Induces Resistance to Leaf Infection by Botrytis cinerea on Bean

Protection of Tomato Seedlings against Infection by Pseudomonas syringae pv. Tomato by Using the Plant Growth-Promoting Bacterium Azospirillum brasilense

Contact Info

Product

Resources

About