Induction of diploid gynogenesis in southern flounder (Paralichthys lethostigma) with homologous and heterologous sperm

Luckenbach, J. Adam; Godwin, John; Daniels, Harry V.; Beasley, Jessica M; Sullivan, Craig V.; Borski, Russell J.

doi:10.1016/j.aquaculture.2004.05.005

Cited by 46 publications

(36 citation statements)

References 53 publications

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“…The highest dosage (150 J cm À2 ) examined eliminated all activity of sperm, while lower treatment levels significantly decreased sperm motility in a dose-dependent manner. Similar cases were also found in southern flounder and black sea bass (Luckenbach et al 2004;Morgan et al 2006). Exposure to UV light reduced the duration of sperm motility in P. crocea, which was similar to findings in European sea bass, southern flounder and black sea bass (Felip et al 1999;Luckenbach et al 2004;Morgan et al 2006).…”

Section: Discussionsupporting

confidence: 85%

“…Similar cases were also found in southern flounder and black sea bass (Luckenbach et al 2004;Morgan et al 2006). Exposure to UV light reduced the duration of sperm motility in P. crocea, which was similar to findings in European sea bass, southern flounder and black sea bass (Felip et al 1999;Luckenbach et al 2004;Morgan et al 2006). The dose-related effect observed with these species may not apply to all fish, for the duration of sperm motility was not responsive to UV dosage in all species.…”

Section: Discussionsupporting

confidence: 85%

“…However, compared with undiluted (''dry'') semen, pre-diluted semen showed reduced motility, as shown in some other studies (Yao et al 1999;Luckenbach et al 2004;Morgan et al 2006). Thinly spread Time after Fertilization (min)…”

Section: Discussionmentioning

confidence: 83%

“…Compared with the most appropriate conditions established for other marine teleost species (Felip et al 2001), the conditions established for P. crocea are different in terms of timing and temperature of the shock, i.e., commencement of shock at 3 min after fertilization and shock temperature of 38C. This is probably due to the fact that other marine fish for which cold shocks have been tested are cultured at 14-168C, as is the case for Japanese flounder, turbot and sea bass (Yamamoto 1999;Piferrer et al 2004;Luckenbach et al 2004), in contrast to the 20-228C for P. crocea culture (Lin et al 1991). With regard to the best shock duration, shocks longer than 12 min did not increase the number of gynogenetic larvae obtained under a given shock temperature and timing.…”

Section: Discussionmentioning

confidence: 96%

“…Thus, a second step is needed to re-establish diploidy in the eggs by blocking the extrusion of the second polar body (meiotic gynogenesis) or by preventing the first embryonic cleavage (mitotic gynogenesis) by an external shock (thermal, pressure, or chemical) (Devlin and Nagahama 2002). Currently, gynogenesis has been induced successfully in many species (Yamamoto 1999;Felip et al 2001;Piferrer et al 2004;Luckenbach et al 2004;Flynn et al 2006). However, as far as we know, the successful production of gynogenetic diploid P. crocea has not yet been reported.…”

Section: Introductionmentioning

confidence: 96%

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Effects of ultra-violet irradiation on sperm motility and diploid gynogenesis induction in large yellow croaker (Pseudosciaena crocea) undergoing cold shock

You

Yan

et al. 2007

Aquacult Int

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Large yellow croaker, Pseudosciaena crocea, exhibit sexually dimorphic growth, with females growing faster and reaching larger adult sizes than males. Thus, development of techniques for preferentially producing females is necessary to optimize production of these species. We have established a protocol to produce all-female croaker P. crocea through induction of meiotic gynogenesis with homologous sperm. The first set of experiments investigated the ultra-violet (UV) irradiation on sperm motility and duration of sperm activity to determine the optimal UV dosage for genetic inactivation of sperm, yet retaining adequate motility for activation of eggs. Milt from several males was diluted 1:100 with Ringer's solution and UV irradiated with doses ranging from 0-150 J cm À2 . The results indicated that motility and duration of activity generally decreased with increased UV doses. At UV doses greater than 105 J cm À2 , after fertilization, motility was <10% and fertilization rates were significantly lower. Highest hatching rate was obtained at 75 J cm À2 . A second set of experiments was carried out to determine appropriate conditions of cold shock for retention of the 2nd polar body in P. crocea eggs after fertilization with UV-inactivated sperm by altering the timing, temperature and duration of shock. At 208C, shock applied at 3 min after fertilization resulted in higher survival rate of larvae at 6 h after hatching. Results of different combinations of three shock temperatures (28C, 38C or 48C) and five shock durations (4 min, 8 min, 12 min, 16 min or 20 min) at 3 min after fertilization demonstrated that shocks of 12 min gave highest production of diploid gynogens. Statistical analysis revealed that maximum production of diploid gynogens (44.55 ± 2.99%) were obtained at 38C. The results of this study indicate that the use of UV-irradiated homologous sperm for activation of P. crocea eggs and cold shock for polar body retention is an effective method for producing gynogenetic offspring.

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