Induction of Diploid Eggs With Colchicine During Embryo Sac Development in Populus

Wang, J.; Kang, Xiangyang; Li, D. L.; Chen, H. W.; Zhang, P. D.

doi:10.1515/sg-2010-0005

Cited by 41 publications

(53 citation statements)

References 21 publications

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“…Reports on triploid production via induction of 2n eggs are rare in Populus, because it is difficult to determine the exact stage of megasporogenesis, which processes inside of ovules of a whole flower (WANG et al, 2010). However, based on the analysis of the temporal relationship between megasporogenesis and microspore development which allowed the optimal time for colchicine treatment to be determined, LI et al (2008) obtained up to 16.7 % triploid plants by inducing unreduced megaspores at the prophase of the first meiotic division in P. alba x P. glandulosa.…”

Section: Use Of Diploid Gametesmentioning

confidence: 99%

Breeding triploid aspen and poplar clones for biomass production

Ulrich

Ewald

2014

Silvae Genetica

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Enriched diploid pollen was applied for in vitro pollinations and crossbreeding in the greenhouse to produce high performance triploid aspen and aspen hybrids for cultivation in medium rotation plantations. In addition to crossings within the section Populus, intersectional crossbreeding was performed to combine benefits of intersectional hybridization with those derived from triploidisation.Both the enrichment of diploid pollen by size fractionation of naturally unreduced pollen and heat treatment of microspore mother cells resulted in a distinct increase of diploid pollen. Using this pollen, six triploid plants were obtained from in vitro pollinations and twenty from crossbreeding in the greenhouse. The triploid plants displayed a high variability in growth performance. Two clones from in vitro pollination and five from crossbreeding in the greenhouse were chosen to estimate growth characteristics. A first assessment of clone performance in an outdoor container test con - ducted over one growing season revealed two triploid clones with a same stem height and a significantly increased basal stem diameter in comparison to the fast-growing triploid reference clone “Astria”. Crossbreeding experiments also resulted in two fast-growing mixoploid clones, which have already been stable for several years.All in all, in this study, crossbreeding using enriched diploid pollen is proved to be a reliable and applicable approach for an effective breeding of triploid poplars.

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Section: Use Of Diploid Gametesmentioning

confidence: 99%

Breeding triploid aspen and poplar clones for biomass production

Ulrich

Ewald

2014

Silvae Genetica

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“…Both colchicine and high temperature are usually used to induce polyploids in plants ( Randolph 1932;Särkilahti and Valanne 1990;Zhang et al 2002;Dhooghe et al 2010). In the genus Populus, colchicine has been used to successfully induce 2n pollen, 2n eggs and somatic chromosome doubling (Mattila 1961;Kang et al 2000;Ewald et al 2009;Wang et al 2010), producing both triploids and tetraploids. Recently, Wang et al (2012a, b) reported 2n female gamete induction with high-temperature treatment during megasporogenesis and embryo sac development, resulting in the production of a number of triploid plants.…”

Section: Discussionmentioning

confidence: 99%

“…P. × canadensis 'Zhonglin-46', P. × canadensis 'Sacrau 79', P. × liaohenica, P. × langfangensis-3 and triploid clones of P. tomentosa, have been widely deployed in artificial plantations because of their good growth properties and pulpwood characteristics. In order to obtain triploid plants of Populus, unilateral sexual polyploidization via induced 2n male or female gametes has been widely studied (Mashkina et al 1989a;Kang et al 2000;Li et al 2008;Wang et al 2010;Wang et al 2012a, b). More than 60% triploid production efficiency can be achieved via hybridization with induced 2n female gametes (Wang et al 2010;Wang et al 2012b).…”

Section: Discussionmentioning

confidence: 99%

“…In order to obtain triploid plants of Populus, unilateral sexual polyploidization via induced 2n male or female gametes has been widely studied (Mashkina et al 1989a;Kang et al 2000;Li et al 2008;Wang et al 2010;Wang et al 2012a, b). More than 60% triploid production efficiency can be achieved via hybridization with induced 2n female gametes (Wang et al 2010;Wang et al 2012b). However, crossing allotetraploids with diploids is still the most direct approach.…”

Section: Discussionmentioning

confidence: 99%

“…Many approaches can be used to produce triploid poplars, e.g. pollination with spontaneous or induced diploid (2n) pollen (Johnsson and Eklundh 1940;Seitz 1954;Mashkina et al 1989a;Zhu et al 1995;Kang et al 2000) and hybridization with natural or artificial 2n female gametes (Li et al 2008;Zhang et al 2009;Wang et al 2010;Wang et al 2012a, b). However, hybridization of tetraploid parents should be the most direct approach because of 2n gamete production from normal meiosis of the allotetraploid.…”

Section: Introductionmentioning

confidence: 99%

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Tetraploid production through zygotic chromosome doubling in <i>Populus</i>

Wang¹,

Shi²,

Song³

et al. 2013

Silva Fenn.

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The most direct approach in breeding triploid Populus is crossing allotetraploids with diploids. However, the lack of allotetraploid Populus restricts application of this approach. In this investigation, zygotic chromosome doubling was induced with colchicine and high temperatures to produce ((Populus pseudo-simonii × P. nigra 'Zheyin3#') × (P. × beijingensis)) allotetraploids. We screened 6 and 25 tetraploid individual offspring from the colchicine and high-temperature treatments respectively, indicating that both colchicine and high temperature are effective for tetraploid production by zygotic chromosome doubling of Populus. Developmental characteristics of seed hairs in the ovaries were temporally associated with zygotic development, which was used to successfully guide the colchicine and high-temperature treatments. During certain stages of hair development, the efficiency of tetraploid production was significantly high. However, efficiency of production was not significantly influenced by other factors, i.e. colchicine concentration, temperature or duration of high-temperature treatment. Size and frequency of leaf stomata between tetraploid and diploid plants were significantly different, suggesting that this character can be altered via genomic increase in material. The allotetraploids produced in this investigation, having different genotypes, provide important parental germplasms for further triploid breeding.

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Inducing triploids and tetraploids with high temperatures in Populus sect. Tacamahaca

Guo

Zhang

et al. 2016

Plant Cell Rep

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This study is the first to report that triploids and tetraploids have been successfully produced through embryo sac and zygotic embryo chromosome doubling with high temperatures in P. simonii Carr. and its hybrid. A new synthetic polyploid induced by hybridization with unreduced gametes and heterozygotic embryo chromosome doubling can effectively combine polyploidy and heterosis, which can provide two major breeding advantages. In Populus, successfully creating and cultivating new polyploid varieties have economic and ecological production value. This was the first successful study in which embryo sac and zygotic embryo chromosome doubling was induced using high temperatures to produce triploids and tetraploids in Populus simonii Carr. and its hybrid, P. simonii × P. nigra var. Italica, of Populus sect. Tacamahaca. The relationship between flower bud morphological characteristics (time after pollination) and female meiotic stage (embryo sac and zygotic embryo development) was established to guide the induction treatment period. In the resulting progeny, 37 triploids and 12 tetraploids were obtained and identified using flow cytometry. The optimal temperatures for embryo sac and zygotic embryo chromosome doubling were 38 and 41 °C, respectively. Cytogenetic analysis revealed that 66-72 h after pollination (HAP), a period characterized by a high proportion of one-nucleate and two-nucleate embryo sacs, was the optimal period for embryo sac chromosome doubling. For zygotic embryo chromosome doubling, 168 HAP was the optimal induction period, as there was a high proportion of two-cell and four-cell proembryos. The results indicate that inducing embryo sac and zygotic embryo chromosome doubling is an ideal method for producing polyploids. The methods for inducing polyploids and for evaluating ploidy and offspring with different ploidies and heterozygosity in this study will be useful for genetic research and Populus breeding programmes.

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Induction of Diploid Eggs With Colchicine During Embryo Sac Development in Populus

Cited by 41 publications

References 21 publications

Breeding triploid aspen and poplar clones for biomass production

Breeding triploid aspen and poplar clones for biomass production

Tetraploid production through zygotic chromosome doubling in <i>Populus</i>

Inducing triploids and tetraploids with high temperatures in Populus sect. Tacamahaca

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