Induction of digestive α-amylases in larvae of Zabrotes subfasciatus (Coleoptera: Bruchidae) in response to ingestion of common bean α-amylase inhibitor 1

Silva, Carlos P.; Terra, Walter R.; Sá, Maria Fátima Grossi de; Samuels, Richard Ian; Isejima, Eliza Mitiko; Bifano, Thomas G.; Almeida, Jonas S.

doi:10.1016/s0022-1910(01)00115-9

Cited by 54 publications

(37 citation statements)

References 33 publications

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“…However, activities in domesticated silkworms ( Bombyx mori ), which are mulberry specialists, are not affected whereas activities in Eri silkworms ( Samia ricini ), which are generalist insect herbivores, were inhibited by very low concentrations of the alkaloids (212). In another example, when larvae of bean weavils ( Zabrotes subfasciatus ) were fed seeds of Phaseolus vulgaris they secreted inducible isoforms of alpha-amylases that were insensitive to the alpha-amylase inhibitor that is found in the plant, whereas their constitutively produced alpha-amylase was inhibited by SMs in the plant [reference (29); see also references (29, 403)]. The entire topic of coevolution of digestive enzymes and plants SMs is not only interesting but also very important, because plant biologists are now experimentally manipulating in crop plants the genes that regulate inhibitory SMs to enhance resistance to crop pests.…”

Section: Interactions Between Naturally Occurring Toxins and Digestivmentioning

confidence: 99%

Comparative Digestive Physiology

Karasov

Douglas

2013

Comprehensive Physiology

257

221

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In vertebrates and invertebrates, morphological and functional features of gastrointestinal (GI) tracts generally reflect food chemistry, such as content of carbohydrates, proteins, fats, and material(s) refractory to rapid digestion (e.g., cellulose). The expression of digestive enzymes and nutrient transporters approximately matches the dietary load of their respective substrates, with relatively modest excess capacity. Mechanisms explaining differences in hydrolase activity between populations and species include gene copy number variations and single-nucleotide polymorphisms. Transcriptional and posttranscriptional adjustments mediate phenotypic changes in the expression of hydrolases and transporters in response to dietary signals. Many species respond to higher food intake by flexibly increasing digestive compartment size. Fermentative processes by symbiotic microorganisms are important for cellulose degradation but are relatively slow, so animals that rely on those processes typically possess special enlarged compartment(s) to maintain a microbiota and other GI structures that slow digesta flow. The taxon richness of the gut microbiota, usually identified by 16S rRNA gene sequencing, is typically an order of magnitude greater in vertebrates than invertebrates, and the interspecific variation in microbial composition is strongly influenced by diet. Many of the nutrient transporters are orthologous across different animal phyla, though functional details may vary (e.g., glucose and amino acid transport with K+ rather than Na+ as a counter ion). Paracellular absorption is important in many birds. Natural toxins are ubiquitous in foods and may influence key features such as digesta transit, enzymatic breakdown, microbial fermentation, and absorption

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Section: Interactions Between Naturally Occurring Toxins and Digestivmentioning

confidence: 99%

Comparative Digestive Physiology

Karasov

Douglas

2013

Comprehensive Physiology

257

221

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“…Inomata et al (1995) studied intraspecific variation of alpha-amylase activity in D. melanogaster and D. immigrans, and suggested that glucose repression was conserved among species at both stages while starch induction was mainly observed in larvae, although the degree of the response depended on species. Silva et al (2001) showed that the alpha-amylase gene of Zabrotes subfasciatus (Boheman 1833) was regulated according to the amount of starch in the food substrate or the amount of glucose, the product of its action. We observed that this type of regulation also exists in P. interpunctella larvae.…”

Section: Discussionmentioning

confidence: 99%

“…Insect development is influenced by different biotic and abiotic factors such as temperature, humidity, photoperiod and the quantity of food (Johnson et al 1992). These parameters also affect the physiological processes of insects (Johnson et al 1997;Na & Ryoo 2000;Musa & Ren 2005), therefore the secretion of digestive enzymes depends on the nature of food or chemical compounds ingested (Mendiola-Olaya et al 2000;Silva et al 2001). Digestive enzymes are regulated by feeding response to nutritional quality and availability of diet (Afshar et al 2010).…”

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confidence: 99%

Effect of four different nutrition regimes on the alpha-amylase gene expression in the Indian moth,Plodia interpunctella

Masoumi

Pourabad

Mohommadi

et al. 2016

Annales de la Société entomologique de France (N.S.)

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Plodia interpunctella (Hübner 1813) (Lepidoptera: Pyralidae) is a cosmopolitan species known to infest a wide range of dried plant materials, including legumes and cereals. In this study, enzyme activity and expression of alphaamylase gene in larval P. interpunctella grown on four different nutrition regimes was studied. The different nutrition regimes caused differential change in expression of alpha-amylase gene. Compared with the control (artificial diet), the walnut regime caused a 10.8% increase in expression of the alpha-amylase gene, whereas 79.3, 52.5, and 7.5% decreases were observed in the larvae grown on dates, raisins and oleaster respectively. The results also showed that glucose repression varied among larvae feeding on different food diets. The results revealed that expression of the alpha-amylase coding gene was significantly reduced in the larvae grown on date and raisin containing high amounts of glucose. A biochemical assay indicated overlapping of alpha-amylase activity and expression. Complexity in expression of amylase genes suggests the existence of a negative feedback mechanism in the insect larvae. Different parameters can affect gene expression and activation, which may explain the results presented here.Résumé. Effet de quatre régimes alimentaires différents sur l'expression du gène de l'alpha-amylase chez la Teigne des fruits secs, Plodia interpunctella. Plodia interpunctella (Hübner 1813) (Lepidoptera : Pyralidae) est une espèce cosmopolite connues pour infester une large gamme de matières végétales séchées, y compris les légumineuses et les céréales. Dans cette étude, l'activité enzymatique et l'expression du gène de l'alpha-amylase dans des larves de P. interpunctella élevées sur quatre régimes alimentaires différents ont été étudiées. Ces différents régimes ont causé un changement important dans l'expression du gène de l'alpha-amylase. Comparativement avec le régime témoin (milieu artificiel), le régime à base de noix a induit une augmentation de 10,8% dans l'expression du gène, tandis que 79,3%, 52,5% et 7,5% de baisse ont été obtenus chez les larves qui ont eu un régime, respectivement, à base de dates, de raisins secs et d'olives. Les résultats ont montré que la répression du glucose variait en fonction du régime alimentaire des larves. Ils ont également révélé que l'expression de gène codant pour l'alpha-amylase a été significativement réduite chez les larves se nourrissant de dates et de raisins secs contenant une grande quantité de glucose. Des tests biochimiques ont indiqué un chevauchement entre l'activité de l'alpha-amylase et son expression. La complexité dans l'expression des gènes de l'amylase suggère l'existence d'un mécanisme de rétroaction négative chez les larves de cet insecte. Différents paramètres peuvent influencer l'expression et l'activation des gènes codant pour l'alpha-amylase et permettent d'expliquer les résultats obtenus.

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“…At least three different isoforms of a-AI including a-AI-1, a-AI-2, and a-AI-3 are reported to be present in the bean seeds of which the most common form is a-AI-1 (Silva et al 2001). It is interesting to find that in the seed extract of bean, no inhibition was found against a-glucosidase of the Sunn pest.…”

mentioning

confidence: 98%

“…Archives of Phytopathology and Plant Protection amylase inhibitors (a-AI) were reported to be present in the bean (Phaseolus vulgaris) (Silva et al 2001;Mehrabadi et al 2012). At least three different isoforms of a-AI including a-AI-1, a-AI-2, and a-AI-3 are reported to be present in the bean seeds of which the most common form is a-AI-1 (Silva et al 2001).…”

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confidence: 99%

Comparison of the effects of three plant protein inhibitors on α-glucosidase of Sunn pest,Eurygaster integricepsPutton

Rahimi

Bandani

2013

Archives Of Phytopathology And Plant Protection

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The Sunn pest is a serious pest of wheat and the other cereals. In this study, the effects of proteinaceous extracts of chickpea, bean and rice against the Sunn pest a-glucosidase was investigated. Results showed that chickpea seed extract has an inhibitory effect on a-glucosidase activity of the Sunn pest gut but bean and rice seed extracts did not affect the enzyme activity. The effects of various concentrations of chickpea seed extract on a-glucosidase activity of gut were dose dependant. There were significant differences between the control and different treatments. Also, results showed that the effect of chickpea seed extracts on the aglucosidase of the Sunn pest gut was pH dependant. Thus, our results showed that chickpea seed extract, but not bean and rice seed extract, has biological activity against the Sunn pest a-glucosidase and it could contribute towards the development of new insect pest control strategies.

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Induction of digestive α-amylases in larvae of Zabrotes subfasciatus (Coleoptera: Bruchidae) in response to ingestion of common bean α-amylase inhibitor 1

Cited by 54 publications

References 33 publications

Comparative Digestive Physiology

Comparative Digestive Physiology

Effect of four different nutrition regimes on the alpha-amylase gene expression in the Indian moth,Plodia interpunctella

Comparison of the effects of three plant protein inhibitors on α-glucosidase of Sunn pest,Eurygaster integricepsPutton

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