Induction of dental pulp stem cell differentiation into odontoblasts by electroporation-mediated gene delivery of growth/differentiation factor 11 (Gdf11)

Nakashima, Mitsuyoshi; Mizunuma, K; Murakami, Teruo; Akamine, Akifumi

doi:10.1038/sj.gt.3301692

Cited by 106 publications

(72 citation statements)

References 32 publications

(32 reference statements)

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“…Modifi cation of DPSCs with GDF11 led to induction of dentin sialoprotein expression, a marker of odontoblasts differentiation, and formation of large amounts of reparative dentine in the pulp isolated from a dog tooth [ 55 ]. The authors obtained similar results by modifying mice DPSCs in vitro and in vivo with GDF11 by electroporation [ 56 ]. These results demonstrate the feasibility of using BMPs in gene-cell applications for endodontic treatment of teeth.…”

Section: Genetic Modifi Cation For Modulation Of Cell Phenotype and Osupporting

confidence: 59%

Genetically Engineered Dental Stem Cells for Regenerative Medicine

2016

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Section: Genetic Modifi Cation For Modulation Of Cell Phenotype and Osupporting

confidence: 59%

Genetically Engineered Dental Stem Cells for Regenerative Medicine

2016

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“…Similar results were obtained with growth differentiation factor 11 delivered by electroporation-mediated gene delivery. 37 Currently, it is not clear whether cytodifferentiation was due only to the physical contact of DMP1 with the undifferentiated cells at the repair site or if DMP1 activated a cascade of events, where the cytodifferentiated cells were able to recruit other cells to the site of injury and induce their differentiation.…”

Section: Dentin Matrix Protein 1 Induces Cytodifferentiation a Almushmentioning

confidence: 99%

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

et al. 2005

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Odontoblasts are postmitotic cells that differentiate from the dental papilla. These cells are responsible for producing the calcified dentin matrix. The pulp-odontoblast interphase contains undifferentiated mesenchymal stem cells, which have the ability to cytodifferentiate into odontoblast-like cells in response to specific signaling molecules. Dentin matrix protein 1 (DMP1) is one of the dentin noncollagenous extracellular matrix proteins that has been implicated in regulation of mineralization. In this study, we have examined the potential role of DMP1 in inducing cytodifferentiation of dental pulp stem cells into odontoblast-like cells and formation of reparative dentin in a rat model. Cavities were drilled and pulps exposed in maxillary first molars. Collagen matrix impregnated with recombinant DMP1 was implanted directly in Group 1, while calcium hydroxide, a commonly used pulp-capping agent was implanted in group 2, collagen matrix that was not impregnated with rDMP1 was implanted directly in group 3, which served as control. Each of these three groups was subdivided into two subgroups, A for 2 weeks time duration and B for 4 weeks duration. At the end of the time period the maxillae were excised, tissues were processed for histological and immunohistochemical evaluations. The results showed that DMP1 could act as a morphogen on undifferentiated mesenchymal cells present in the dentin-pulp complex. These differentiated cells had the potential to regenerate dentin-like tissue, which was confirmed by the presence of collagenous matrix, odontoblast specific markers and calcified deposits. Gene Therapy (2006) 13, 611-620.

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“…When exogenous Gdf-11 gene is imported into DPSCs by electrical transfection method, it can trigger the odontoblastic differentiation of these stem cells and bring about the formation of dentin-like structures [69]. The genes related to odonto/osteo-blastic differentiation (Alp, Ocn, Col-1, Bsp, and Dspp) can be up-regulated when Bmp-2 gene is transferred into STRO-1 + DPSCs [47].…”

Section: Differentiation Of Dpscs Into Odontoblastsmentioning

confidence: 99%

“…Many studies have shown that DPSCs play a paramount role in the dentin-pulp tissue regeneration [1,2,9,12,29,[69][70][71][72][73][74]. Gronthos et al have recombined human DPSCs with hydroxyapatite-tricalcium phosphate (HA-TCP) ceramic powder, and transplanted them subcutaneously into immunocompromised mice.…”

Section: Regeneration Of Dentin-pulp Complex By Dpscsmentioning

confidence: 99%

A Journey from Dental Pulp Stem Cells to a Bio-tooth

Yan

Zhang

et al. 2010

Stem Cell Rev and Rep

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The ultimate goal of tooth regeneration is to replace the lost teeth. Stem cell-based tooth engineering is deemed as a promising approach to the making of a biological tooth (bio-tooth). Dental pulp stem cells (DPSCs) represent a kind of adult cell colony which has the potent capacity of self-renewing and multilineage differentiation. The exact origin of DPSCs has not been fully determined and these stem cells seem to be the source of odontoblasts that contribute to the formation of dentin-pulp complex. Recently, achievements obtained from stem cell biology and tooth regeneration have enabled us to contemplate the potential applications of DPSCs. Some studies have proved that DPSCs are capable of producing dental tissues in vivo including dentin, pulp, and crown-like structures. Whereas other investigations have shown that these stem cells can bring about the formation of bone-like tissues. Theoretically, a bio-tooth made from autogenous DPSCs should be the best choice for clinical tooth reconstruction. This review will focus on the location, origin, and current isolation approaches of these stem cells. Their odontoblastic differentiation and potential utilizations in the reconstruction of dentin-pulp complex and bio-tooth will be extensively discussed.

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Induction of dental pulp stem cell differentiation into odontoblasts by electroporation-mediated gene delivery of growth/differentiation factor 11 (Gdf11)

Cited by 106 publications

References 32 publications

Genetically Engineered Dental Stem Cells for Regenerative Medicine

Genetically Engineered Dental Stem Cells for Regenerative Medicine

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

A Journey from Dental Pulp Stem Cells to a Bio-tooth

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