Induction of Defense Responses in Cucumber Plants by Using the Cell-free Filtrate of the Plant Growth-Promoting Fungus Penicillium simplicissimum GP17-2

Shimizu, Kaori; Hossain, Md. Motaher; Kato, Kimihiko; Kubota, Mashaharu; Hyakumachi, Mitsuro

doi:10.5650/jos.62.613

Cited by 24 publications

(8 citation statements)

References 49 publications

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“…A spore suspension 10 5 spores mL 1 of the anthracnose pathogen C. orbiculare isolate 104T was prepared as described by Chandanie et al 22 . The challenge inoculation and subsequent disease assessment of the total number of lesions and area mm 2 of each lesion developed by C. orbiculare were performed in a similar manner to Shimizu et al 23 .…”

Section: Bgi Experimentsmentioning

confidence: 99%

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The Plant Growth-promoting Fungus Penicillium spp. GP15-1 Enhances Growth and Confers Protection against Damping-off and Anthracnose in the Cucumber

et al. 2014

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Section: Bgi Experimentsmentioning

confidence: 99%

“…The induction treatment with the CF of GP15-1, the challenge inoculation with the spore suspension of C. orbiculare, and the subsequent disease assessment were carried out in a similar manner to Shimizu et al 23 .…”

Section: Cf Experimentsmentioning

confidence: 99%

The Plant Growth-promoting Fungus Penicillium spp. GP15-1 Enhances Growth and Confers Protection against Damping-off and Anthracnose in the Cucumber

et al. 2014

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“…In recent years, several PGPF Penicillium spp. have been extensively tested for their ability to exert beneficial effects on plants using different forms of inocula (Elsharkawy, Shimizu, Takahashi, & Hyakumachi, 2012;Shimizu, Hossain, Kato, Kubota, & Hyakumachi, 2013;Wakelin, Gupta, Harvey, & Ryder, 2007) . These works have established the potential for use of Penicillium inoculants to increase the growth of various plants.…”

Section: Introductionmentioning

confidence: 99%

Role of ethylene signalling in growth and systemic resistance induction by the plant growth‐promoting fungus Penicillium viridicatum in Arabidopsis

Hossain

Sultana

Hyakumachi

2017

Journal of Phytopathology

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The plant growth‐promoting fungi (PGPF) have long been known to improve plant growth and suppress plant diseases. The PGPF Penicillium viridicatum GP15‐1 elicited plant growth and induced systemic resistance (ISR) in Arabidopsis thaliana against Pseudomonas syringae pv. tomato DC3000 (Pst), leading to a restriction of pathogen growth and disease development. Examination of local and systemic genes indicated that GP15‐1 did not modulate the expression of any of the tested defence‐related marker genes involved in salicylic acid (SA), jasmonic acid (JA) and ethylene signalling pathways. Subsequent challenge of GP15‐1‐colonized plants with Pst bacterium primed Arabidopsis plants for enhanced activation of the JA‐inducible Atvsp (vegetative storage protein) gene at a later stage of infection. To assess the contribution of different signalling pathways in GP15‐1‐elicited plant growth and ISR, Arabidopsis genotypes implicated in SA signalling expressing the nahG transgene (NahG) or carrying disruption in NPR1 (npr1), JA signalling (jar1) and ethylene signalling (ein2) were tested. The GP15‐1‐induced plant growth and ISR were fully compromised in an ein2 mutation. Root colonization assay revealed that the inability of the ein2 mutant to express GP15‐1‐induced plant growth and ISR was not associated with reduced root colonization by GP15‐1. In conclusion, our results demonstrate the ethylene signalling pathway is involved in plant growth promotion and ISR elicitation by the PGPF P. viridicatum GP15‐1 in Arabidopsis. These results provide evidence that ethylene signalling has a substantial role in plant growth and disease resistance.

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“…PGPF activate plant defense response against different pathogens through ISR (Meera et al 1994b(Meera et al , 1995a(Meera et al , 1995bChandanie et al 2006Chandanie et al , 2009Elsharkawy et al 2012aElsharkawy et al , 2012bElsharkawy et al , 2013Shimizu et al 2013;Sudisha et al 2013). To understand the mech-tested the role of β-1,3-gluconases, chitinases, PO, polyphenol oxidase (PPO), and phenylalanine ammonia lyase (PAL) in the defense mechanism of cucumber plants, following systemic induction with PGPF and challenging with Colletotrichum orbiculare (Berk.& Mont) von Arx.(syn.…”

Section: Introductionmentioning

confidence: 99%

Elevated expression of hydrolases, oxidase, and lyase in susceptible and resistant cucumber cultivars systemically induced with plant growth-promoting fungi against anthracnose

Hassan

Elsharkawy

Shivanna

et al. 2014

Acta Agriculturae Scandinavica, Section B — Soil & Plant Sc

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Susceptible and resistant cultivars of cucumber (cv. Suyo and cv. Shogoin fushinari, respectively) were evaluated for induction of systemic resistance by plant growth-promoting fungal isolates Phoma sp. GS8-1 and nonsporulating fungus GU21-2 against Colletotrichum orbiculare. Reduction in anthracnose disease was assessed at regular intervals of 0, 3, 6, and 9 days after challenge inoculation. Activities of endochitinase, β-1, 3-endoglucanase, peroxidase (PO), polyphenol oxidase, and phenylalanine ammonia lyase (PAL) were assayed at the above intervals, while the activities of exochitinase and β-1,3-exogluconase were assayed at 9 days after challenge inoculation. The data showed that all the enzymes activities increased in plants treated with plant growth-promoting fungi (PGPF) compared with nontreated plants of both cultivars. The activities of endochitinase and β-1,3-endoglucanase were highly increased in PGPF-induced plants than in nontreated plants of both cultivars at different time points. Activities of PO, polyphenol oxidase, and PAL also increased between 3 and 6 days due to inoculation with PGPF and decreased thereafter. The activity of PAL at 9 days in PGPF-induced plants was at the same level as that of nontreated plants of both cultivars. Results of this investigation suggest that PGPF-induced systemic resistance is associated with not only high increases in the activities of chitinase and β-1,3-gluconases but also other plant defense-related enzymes such as PO, PPO, and PAL. Roles of other mechanisms rather than accumulation of defense-related enzymes in PGPF-mediated induced resistance in susceptible cultivars were demonstrated.

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Induction of Defense Responses in Cucumber Plants by Using the Cell-free Filtrate of the Plant Growth-Promoting Fungus Penicillium simplicissimum GP17-2

Cited by 24 publications

References 49 publications

The Plant Growth-promoting Fungus Penicillium spp. GP15-1 Enhances Growth and Confers Protection against Damping-off and Anthracnose in the Cucumber

The Plant Growth-promoting Fungus Penicillium spp. GP15-1 Enhances Growth and Confers Protection against Damping-off and Anthracnose in the Cucumber

Role of ethylene signalling in growth and systemic resistance induction by the plant growth‐promoting fungus Penicillium viridicatum in Arabidopsis

Elevated expression of hydrolases, oxidase, and lyase in susceptible and resistant cucumber cultivars systemically induced with plant growth-promoting fungi against anthracnose

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