Induction of D-xylose uptake and expression of NAD(P)H-linked xylose reductase and NADP¿+¿-linked xylitol dehydrogenase in the oleaginous microalga Chlorella sorokiniana

Abstract

Background: The heterotrophic and mixotrophic culture of oleaginous microalgae is a promising process to produce biofuel feedstock due to the advantage of fast growth. Various organic carbons have been explored for this application. However, despite being one of the most abundant and economical sugar resources in nature, D-xylose has never been demonstrated as a carbon source for wild-type microalgae. The purpose of the present work was to identify the feasibility of D-xylose utilization by the oleaginous microalga Chlorella sorokiniana. Results: The sugar uptake kinetic analysis was performed with

Chlorella minutissima grown with xylose and arabinose in tubular photobioreactors: Evaluation of kinetics, carbohydrate production, and protein profile

“…Reports on the use of D-xylose indicated that better utilization of this sugar is attributed to additional chemical energy derived from light-dependent reactions due to an increase in the generation of NADPH. [8] The ability to attain higher cellular concentrations is one of the numerous advantages of vertical tubular photobioreactors. Even the lowest cell concentrations observed in the treatments at a luminous intensity of 10.13 μmol m −2 s −1 were close to or even higher than the values obtained in the raceway photobioreactors (at the lowest intensity of available light of 8.44 μmol m −2 s −1 ).…”

“…The metabolic pathway for the use of D-xylose by microalgae was described in Chlorella sorokiniana. [8] Subsequently, the effects of xylose and arabinose on the accumulation of lipids in Chlorella and Scenedesmus cells were attributed to the addition of low concentrations of xylose. [9] The addition of arabinose to Chlorella minutissima cells grown in the raceway type reactors stimulated an increase in the production of carbohydrates.…”