Induction of cystic ovarian follicles (COFs) in cattle by using an intrafollicular injection of indomethacin

Lapp, Rebecca; Röttgen, Volker; Viergutz, Torsten; Weitzel, Joachim M.; Vernunft, Andreas

doi:10.1262/jrd.2019-107

Cited by 6 publications

(15 citation statements)

References 35 publications

(73 reference statements)

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“…Indomethacin reliably blocked the ovulation of preovulatory follicles at low doses, leading to ovarian cyst development. Based on these results, a model for inducing artificial ovarian cysts in cattle has already been established [ 28 ]. Indomethacin reportedly blocks ovulation after administration directly into the ovarian tissue of cattle [ 31 ] or other species [ 9 , 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, further increasing the doses for the in vivo experiments did not seem beneficial. It has been hypothesized that the development of an ovarian cyst could be caused by disturbed or continuing estradiol production [ 28 , 44 ]. However, no relationship was observed between PGE 2 reduction (by COX inhibitor treatment) and estradiol production in this study.…”

Section: Discussionmentioning

confidence: 99%

“…FF was cooled immediately on ice, centrifuged at 500 × g for 10 min at 4°C to remove blood or cell debris, and stored at –20°C until analysis. The results from follicle aspirations were already partly used in a technology report [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

“…2). The indomethacin experiments in the subproject [ 28 ] were temporarily carried out in parallel with the other COX inhibitor experiments using the same materials, methods, and study designs.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies demonstrated relationships between biomarkers of oxidative stress (lipohydroperoxides, 8-iso-PGF 2α ) and ovarian function in dairy cows and their direct interference with granulosa cell function [ 26 , 27 ]. Recently, we demonstrated that intrafollicular administration of indomethacin, a nonselective COX-1 and -2 inhibitor, can prevent ovulation in a bovine model [ 28 ]. Moreover, the unruptured follicle further developed into a cystic ovarian follicle.…”

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confidence: 99%

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Effects of different cyclooxygenase inhibitors on prostaglandin E<sub>2</sub> production, steroidogenesis and ovulation of bovine preovulatory follicles

et al. 2022

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Ovulation is an inflammation-like process, and cyclooxygenase-2 (COX-2)-dependent production of prostaglandin E 2 (PGE 2 ) is its key mediator. Balanced regulation of inflammatory processes in high-yielding dairy cows may be essential for physiological ovulation and fertility. This study aimed to elucidate the mechanisms underlying ovulation failure and cyst development after disturbing intrafollicular inflammatory cascades. Therefore, nonselective (indomethacin and flunixin-meglumine), COX-2 selective (meloxicam), and highly COX-2 selective (NS-398) inhibitors were injected into preovulatory follicles 16 h after administration of GnRH, and ovulation was monitored via ultrasound examination. Additionally, follicular fluid was collected after injection of indomethacin, meloxicam, and NS-398. Moreover, primary granulosa cell cultures from preovulatory follicles were prepared and treated with indomethacin, meloxicam, and NS-398. The concentrations of 17β-estradiol, progesterone, and prostaglandin E 2 (PGE 2 ) in the follicular fluid and cell supernatant were estimated. Indomethacin and flunixin-meglumine blocked ovulation, even at low doses, and led to ovarian cyst development. The selective and highly selective COX-2 inhibitors meloxicam and NS-398 were not effective in blocking ovulation. However, indomethacin, meloxicam, and NS-398 significantly and comparably reduced PGE 2 concentration in vivo and in vitro (P < 0.05) but had no effect on estradiol or progesterone production. This may contradict the generally accepted hypothesis that PGE 2 is a key mediator of ovulation and progesterone production. Our results suggest a connection between ovarian disorders and inflammatory actions in early postpartum cows.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of different cyclooxygenase inhibitors on prostaglandin E<sub>2</sub> production, steroidogenesis and ovulation of bovine preovulatory follicles

et al. 2022

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Downregulation of TASK-3 Channel Induces Senescence of Granulosa Cells in the Bovine Follicular Cystic Ovary

Kim,

Woo

et al. 2024

Preprint

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Ovarian cysts are linked to hormone imbalances and altered gene expressions, but the connection between cysts and ion channel expression is understudied. This study explored the role of TWIK-related acid-sensitive K+ (TASK) channels in bovine ovarian cyst formation. The ovarian follicles were split into small (5 to 10 mm in diameter) and large (> 25 mm in diameter) groups. Among the measured K+, Na+, and Cl- concentrations in follicular fluid (FF) obtained from small-sized follicles (SF) and large-sized follicles (LF), the K+ concentration was significantly lower in LFFF. Quantitative PCR, western blot, and immunocytochemistry data revealed that TASK-3 expression levels significantly decreased by approximately 50% in both LF and granulosa cells obtained from LF (LFGCs) compared to the corresponding controls. The TASK-3 protein was localized to the plasma membrane of GCs. The diameter of LFGCs was larger than that of SFGC. The cell swelling response to exposure to a hypotonic solution (200 mOsm/L) was highly reduced in TASK-3-overexpressing cells compared to vector-transfected cells. TASK-3-knockdown cells showed arrested growth. Senescence markers were detected in LFGCs and TASK-3-knockdown cells. These findings suggest that reduced TASK-3 expression in LF is associated with the inhibition of GC growth, leading to senescence and cyst formation.

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A histological pattern of the internal genitalia of cows with follicular ovarian cyst

et al. 2022

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Intensification of the cattle population reproduction is an indispensable condition for the increase in the production of cattle breeding products. However, everyday abnormal effects of various factors on the intensively functioning organism of animals lead to the emergence of pathologies that restrain the rate of increase in the number of cows. Despite a wide range of problems, the results of numerous studies confirm and emphasize that a significant cause of symptomatic infertility in rejected cows is ovarian follicular cysts, accompanied with pathological changes in other organs of the reproductive system. Transrectal and ultrasound examination allows to diagnose pathology, but the issue of changes at the microscopic level in the ovaries and other organs of the reproductive system remains insufficiently studied, which inhibits the development of new methods of restoring the endocrine function of the gonads. In this regard, the morphology and pathology of the ovaries and uterus of cows require further careful study. The research was conducted in the conditions of the agricultural production cooperative “Agrofirma Semenivska”, Sumy region, on Holstein cows of different ages and productivity. At the gynecological stage of dispensation, the condition of the internal genital organs of 47 infertile cows, 14 of which were rejected, and 8 diagnosed with ovarian follicular cyst, was examined. In the conditions of the meat processing plant, the uterus and ovaries were dissected from the genitals taken immediately after slaughtering 8 cows. Pieces of tissue measuring 1 x 2 cm were cut from the walls of the uterus, fallopian tubes, and ovaries, then fixed in 10 % aqueous formalin solution; histosections were made according to the generally accepted method, being stained with hematoxylin-eosin, and examined by light microscopy. It has been established that on the histopreparations, made from the ovary with a follicular cyst, there is desquamation of individual cells of the covering epithelium, atresia of the obliterating type, which is characterized by dystrophic changes of granulosis with pronounced desquamation of follicular cells filling the cavity of the cystic follicle with the subsequent formation of a fibrous atretic body. While examining histological sections from the uterus, a large number of arterial vessels with thick walls surrounded by connective tissue fibers and groups of endometrial glands of a typical structure, which is typical for endometriosis, have been found in the thickness of the myometrium. The endometrium has turned out to be represented by a cytogenic stroma, the density of the cells of which gradually decreases from the surface to the muscle layer, in the basal layer, the stroma is almost entirely replaced by connective tissue. Behind the follicular cyst of the ovary, traces of mucus have been found in the lumen of the fallopian tube.

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Induction of cystic ovarian follicles (COFs) in cattle by using an intrafollicular injection of indomethacin

Cited by 6 publications

References 35 publications

Effects of different cyclooxygenase inhibitors on prostaglandin E<sub>2</sub> production, steroidogenesis and ovulation of bovine preovulatory follicles

Effects of different cyclooxygenase inhibitors on prostaglandin E<sub>2</sub> production, steroidogenesis and ovulation of bovine preovulatory follicles

Downregulation of TASK-3 Channel Induces Senescence of Granulosa Cells in the Bovine Follicular Cystic Ovary

A histological pattern of the internal genitalia of cows with follicular ovarian cyst

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