Induction of cutinolytic esterase activity during saprophytic growth of cucurbit pathogens, Fusarium solani f. sp. cucurbitae races one and two (Nectria haematococca MPI and MPV, respectively)

Abstract

Cutins from fruit of Cucurbita maxima and Cucurbita moschata cultivars, apple and a C 16 alcohol (hexadecanol) were used to induce cutinolytic esterase activity during saprophytic growth of strains of the two cucurbit pathogens, Fusarium solani f. sp. cucurbitae, race 1 (Nectria haematococca mating population (MPI) and F. solani f. sp. cucurbitae, race 2 (MPV). Four strains of MPV and 11 strains of MPI were were included in the study. Although we were primarily interested in the two cucurbit pathogens (MPI and MPV), six strains of the pea pathogen F. solani f. sp. pisi (MPVI) were included to provide a comparison since most of the knowledge on cutinase activity in N. haematococca has come from a study of that group. Cutinolytic esterase was induced in all strains from both MPV and MPVI but was not detected in any of the 11 strains from MPI regardless of the induction conditions. The amount of cutinolytic esterase activity induced in the MPV strains differed according to the strain and both the source and the amount of cutin used in the induction medium. Information on the influence of cutin source and pH on the induction of cutinolytic esterase activity during saprophytic growth of strains from MPV demonstrates that the gene is regulated differently from that in MPVI. ß

Reducción de astringencia del pseudofruto de marañón (Anacardium occidentale L.) como alternativa para consumo humano

“…Degani (2015) reported that cutinase activity increases according to the amount of cutin added to the culture media. Hawthorne et al (2001) demonstrated that the enzymatic activity is regulated by the source and concentration of cutin, which is in accord with the results shown here, since F. culmorum cutinolytic activity raised by increasing cutin concentration. Macedo & Pio (2005) reported that F. oxysporum showed an increase in the cutinolytic activity determined by spectrophotometry using pNPB when a concentration of 4 g of cutin/L was added to the media.…”

The Fusarium solani species complex: ubiquitous pathogens of agricultural importance

“…The induction of cutinase activity in FSSC 1 is dissimilar to that observed in FSSC 11, and therefore it appears that the regulation of cutA in FSSC 1 is different from that of cut1 in FSSC 11 (Hawthorne et al ., ). Another cucurbit pathogen in the FSSC (FSSC 10) lacked cutinase activity, which may explain the difference in tissue specificity between these phylogenetically distinct cucurbit pathogens (Hawthorne et al ., ).…”

“…As with FSSC 11, cutA is induced in the presence of cutin from various cucurbits (Hawthorne et al ., ); however, disruption of the cutA gene in FSSC 1 did not cause a significant reduction in virulence on intact fruit of several varieties of Cucurbita maxima and C. moschata (Crowhurst et al ., ). The induction of cutinase activity in FSSC 1 is dissimilar to that observed in FSSC 11, and therefore it appears that the regulation of cutA in FSSC 1 is different from that of cut1 in FSSC 11 (Hawthorne et al ., ). Another cucurbit pathogen in the FSSC (FSSC 10) lacked cutinase activity, which may explain the difference in tissue specificity between these phylogenetically distinct cucurbit pathogens (Hawthorne et al ., ).…”

Pseudozyma jejuensissp. nov., a novel cutinolytic ustilaginomycetous yeast species that is able to degrade plastic waste

“…phyllosphere-inhabiting fluorescent Pseudomonas putida and Pseudomonas mendocina, cohabiting with nitrogen-fixing bacteria, and Corynebacterium sp.) (Purdy & Kolattukudy, 1975;Kolattukudy, 1984;Dantzig et al, 1986;Sebastian et al, 1987;Petersen et al, 1997;Cristina et al, 1999;Hawthorne et al, 2001;Chen et al, 2006). Boekhout et al (2006) reported that cutinolytic ustilaginomycetous fungi belonging to the genus Tilletiopsis caused a postharvest disease of apples.…”