Induction of Corneal Epithelial Differentiation of Induced Pluripotent and Orbital Fat-Derived Stem Cells Seeded on Decellularized Human Corneas

Martins, Thaís Maria da Mata; Carvalho, Juliana Lott; Cunha, Pricila da Silva; Gomes, Dawidson Assis; Góes, Alfredo M.

doi:10.1007/s12015-022-10356-6

Cited by 2 publications

(3 citation statements)

References 90 publications

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“…Similar results were seen in vivo where LC-COMatrix showed excellent bio integration, replaced partial corneal stromal defects while also closing large and orbital fat-derived (OFSCs) stem cells to CEs. 25 DCs also supported successful adhesion and survival of both cell types while only hiPSCs gave rise to cells expressing important CE markers in vitro.…”

Section: T a B L E 1 Summary Of Cell Instructive Biomaterials Used In...mentioning

confidence: 92%

“…Hence, DCs show great potential to reduce the risk of immune rejection while utilizing invaluable donor tissue. DCs promoted differentiation of induced pluripotent (iPSCs) and orbital fat‐derived (OFSCs) stem cells to CEs 25 . DCs also supported successful adhesion and survival of both cell types while only hiPSCs gave rise to cells expressing important CE markers in vitro.…”

Section: Ophthalmologymentioning

confidence: 99%

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Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Adhikari

Stager

Krebs

2023

J Biomedical Materials Res

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The field of biomaterials aims to improve regenerative outcomes or scientific understanding for a wide range of tissue types and ailments. Biomaterials can be fabricated from natural or synthetic sources and display a plethora of mechanical, electrical, and geometrical properties dependent on their desired application. To date, most biomaterial systems designed for eventual translation to the clinic rely on soluble signaling moieties, such as growth factors, to elicit a specific cellular response. However, these soluble factors are often limited by high cost, convoluted synthesis, low stability, and difficulty in regulation, making the translation of these biomaterials systems to clinical or commercial applications a long and arduous process. In response to this, significant effort has been dedicated to researching cell‐directive biomaterials which can signal for specific cell behavior in the absence of soluble factors. Cells of all tissue types have been shown to be innately in tune with their microenvironment, which is a biological phenomenon that can be exploited by researchers to design materials that direct cell behavior based on their intrinsic characteristics. This review will focus on recent developments in biomaterials that direct cell behavior using biomaterial properties such as charge, peptide presentation, and micro‐ or nano‐geometry. These next generation biomaterials could offer significant strides in the development of clinically relevant medical devices which improve our understanding of the cellular microenvironment and enhance patient care in a variety of ailments.

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Section: T a B L E 1 Summary Of Cell Instructive Biomaterials Used In...mentioning

confidence: 92%

Section: Ophthalmologymentioning

confidence: 99%

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Adhikari

Stager

Krebs

2023

J Biomedical Materials Res

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“…Martins et al [ 29 ] also found that stem cells could not differentiate into corneal cells when OFSCs were transplanted into decellularized corneal stroma, which indicated that direct contact with corneal epithelial cells was a necessary condition for inducing OFSCs to differentiate into corneal epithelial cells. Some scholars [ 30 , 31 ] found that animal models with corneal damage can be recovered to some extent by local instillation of OFSCs mixture or injection of regenerated corneal epithelial cells after mixed culture of OFSCs and corneal epithelial cells in vitro into damaged cornea.…”

Section: Orbital Fat-derived Stem Cells (Ofscs)mentioning

confidence: 99%

Research progress of orbital fat in histology and cytology: A review

Li,

Gao,

Gao

2024

Medicine

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Orbital fat is an adipose tissue located behind orbital septum and originates from mesoderm and neural crest in ectoderm. It has been found that the histologic structure of orbital fat is different from subcutaneous and visceral fat. In addition, the regeneration and anti-inflammatory ability of stem cells derived from orbital fat have attracted much attention in recent years. This paper reviews the recent research progress on orbital fat, including its structure, origin, histological characteristics, and related stem cells.

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Induction of Corneal Epithelial Differentiation of Induced Pluripotent and Orbital Fat-Derived Stem Cells Seeded on Decellularized Human Corneas

Cited by 2 publications

References 90 publications

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Research progress of orbital fat in histology and cytology: A review

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