Induction of collagenolytic and proteolytic activities by anti-inflammatory drugs in the skin and fibroblast

Houck, J. C.; Sharma, V. K.; Patel, Y. M.; Gladner, Jules A.

doi:10.1016/0006-2952(68)90182-2

Cited by 57 publications

(14 citation statements)

References 15 publications

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“…The ECM is a dynamic niche involved in cancer progression (34) and adult stem cells secrete a number of matrix molecules that appear to be important for tissue growth regulation. It is well-known that the stress-induced secretion of cortisol disrupts the ECM (35) and that the SRM may mitigate the effects of stress (36). Furthermore, in the stress pathway, stem cells are inhibited from being activated and released into the damaged tissue (37,38).…”

Section: Slowing the Aging Process Of Stem Cellsmentioning

confidence: 99%

Enhancing spontaneous stem cell healing (Review)

Maguire¹,

Friedman²

2014

Biomedical Reports

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Section: Slowing the Aging Process Of Stem Cellsmentioning

confidence: 99%

Enhancing spontaneous stem cell healing (Review)

Maguire¹,

Friedman²

2014

Biomedical Reports

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“…Finally, Houck et al (8) demonstrated that cortisol, prednisolone, indomethacin, or oxyphenbutazone can, after a single oral dose of 3 mgjkg, induce a significant increase in collagenase or protease activity in rat skin. Prednisone or cortisone were ineffective in the latter experiments.…”

Section: Bmentioning

confidence: 99%

“…The pertinence of the data reported herein, therefore, using bacterial collagenase, must await further clarification of the mechanism and structure of other "collagenases." The experiments of Houck (8) also raise the question of whether the correla-tion between augmented collagenase levels in cultured synoviocytes and the severity of rheumatoid arthritis results from the pathology or the pharmacodynamics of the situation.…”

Section: Bmentioning

confidence: 99%

Inhibition of Elastase and Collagenase by Anti-Inflammatory Drugs

Brown

Pollock

1970

Experimental Biology and Medicine

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Polymorphonuclear leukocytic granules contain elastase and collagenase activities, both of which may be involved in the lysis of vascular basement membranes at physiologic pH ( 1 , 2 ) and, therefore, related to the pathogenesis of vasculitis (2). Abnormally high levels of collagenase are found in synoviocytes of patients with rheumatoid arthritis in tissue culture; levels of the latter correlate positively with the severity of the disease ( 3 ) . Collagenase or elastase inhibitors are, therefore, of potential therapeutic utility, especially since these may attenuate the development or perpetuation of connective tissue diseases. The purpose of this report is to describe the effects of currently used antiinflammatory agents on crystalline pancreatic elastase and C. histolyticum collagenase, both of which are similar to the abovementioned leukocytic or synovial enzymes.Materials and Methods. Achilles tendon collagen and chromatographically purified collagenase (C. histolyticum) were purchased from Worthington Biochemical Corporation. Twice-crystallized pancreatic elastase (porcine) and elastin-orcein were obtained from Sigma Chemical C,ompany. Collagenase was assayed by incubating 2 pg of enzyme and 25 mg collagen in 0.025 IM Tris-HC1 buffer, pH 8.0, with or without drugs for 20 hr at 37", followed by filtration through Whatman No.40 filter paper. The total reaction volume was 3.0 ml; test tubes were covered with parafilm to avoid evaporation. The filtrate (0.2 ml) was assayed for liberation of amino acids by the ninhydrin procedure of Moore and Stein (4) ; leucine was employed as a reference amino acid. Blank (no enzyme) and control (enzyme, no drug) absorbance values were, respectively, 0.126 & .005 ( n = 15) and 0.611 t .0826 ( n = 2 2 ) . Collagenase (4 Lozdsiana 70112 pg) was also assayed using 20 mg of insoluble diazotized collagen (azocoll, Calbiochem) as substrate in 2.0 ml 0.025 M Tris-HC1 buffer, pH 8.0, 37', for 30 min followed by addition of 3.0 ml distilled water and filtration through Whatman No. 40 filter paper.Absorbance was determined at 520 mp. Elastase activity was measured by incubating 0.1 mg/ml enzyme in a 2.0-ml total reaction volume containing 20 mg elastin-orcein and 0.025 M Tris-HC1 buffer, pH 8.0, with or without drugs for 60 min at 37". Reaction was terminated by addition of 3.0 ml distilled water followed by filtration through Whatman No. 40 or 43 filter paper. Absorbance was determined at 590 mp. Blank (no enzyme) and control (enzyme, no drug) absorbance values were, respectively, 0.027 t .002 ( n = 3) and 0.204 t .006 ( n = 9). Drug solutions were prepared by dissolution in a small amount of 1.0 N sodium hydroxide, dilution to slightly less than 50 ml, neutralization with 1.0 N hydrochloric acid, and final dilution in 0.05 M Tris-HC1 buffer, pH 8.0, to yield the final drug solution a t p H 8.0 in 0.025 M Tris buffer. Addition of sodium hydroxide did not appreciably aid the dissolution of cortisone acetate or dexamethasone. Thus, the latter drugs were slowly triturated into solution. T...

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“…Pentland [15]. The rationale behind the intralesional injection was that the steroids may have an increase in collagenolysis [16]. When the mass becomes too large to undergo injections or conservative therapy has failed and surgery excision becomes warranted there are reports of high recurrence of the mass.…”

Section: Introductionmentioning

confidence: 99%

Multinodular interdigitating plantar fibroma: A case study and literature review

Graney¹,

Baxter²

2017

Surg Rehabil

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Plantar fibromas are a common soft tissue mass that is frequently encountered by a foot and ankle surgeon. Plantar fibromas are typically thought of as a benign, firm, single or multinodular mass within the plantar fascia. There are several additional medical conditions that have been said to be affiliated with plantar fibromas including Dupuytren's contracture, Peyronie's disease, and even epilepsy. Plantar fibromas have been well documented in the past regarding surgical versus nonsurgical treatment as well as complications due to a high recurrence rate. The purpose of this case study and literature review is to evaluate the tumor staging in regards to surgical complications, imaging, recurrence, and histological findings upon excision, as well as present a rare case of an advanced interdigitating multinodular plantar fibroma.

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Induction of collagenolytic and proteolytic activities by anti-inflammatory drugs in the skin and fibroblast

Cited by 57 publications

References 15 publications

Enhancing spontaneous stem cell healing (Review)

Enhancing spontaneous stem cell healing (Review)

Inhibition of Elastase and Collagenase by Anti-Inflammatory Drugs

Multinodular interdigitating plantar fibroma: A case study and literature review

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