Induction of chromosome changes in Chinese hamster cells by exposure to asbestos fibres

Sincock, A. M.; Seabright, Marina

doi:10.1038/257056a0

Cited by 125 publications

(24 citation statements)

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“…In contrast, a comprehensive study by Sincock et al (35) showed that neither cultured human lymphoblasts nor human fibroblasts exhibited chromosomal aberrations when exposed to either UICC crocidolite, SFA chrysotile, or glass fibers (10 ,ug/mL to 100 ,ug/mL, 48-72 hr). In contrast, CHO cells exposed under this same regime exhibited numerous chromosomal changes (35), a result in concordance with earlier studies (30). Both CHO and human cell lines are inhibited to a similar extent with respect to rate of growth by these fiber preparations (35).…”

supporting

confidence: 76%

“…Identical results were obtained by exposing the cells to the fibers for 5 days before the cytogenetic analysis (30). If the fibers were preexposed to complete culture media (with serum), their clastogenic potential was reduced (30).…”

mentioning

confidence: 85%

“…Lavappa et al (31) reported that a sample of Rhodesian chrysotile A (probably UICC) produced chromosomal aberrations (primarily chromatid breaks) in a dose-related manner in a Syrian hamster embryo (SHE) when applied at 0.1 to 100 ,ug/mL for 6 to 96 hr. The preponderance of chromatid damage led the investigators to propose that the G2 was the most sensitive phase of the mitotic cycle to asbestos fiber clastogenic effects (30). It is noteworthy that these same fiber preparations, when administered in vivo, did not induce either chromosomal aberrations (monkey, oral administration) or micronuclei (mouse, intraperitoneal administration) in bone marrow cells (31).…”

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confidence: 99%

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In Vitro Assessment of Asbestos Genotoxicity

Daniel¹

1983

Environmental Health Perspectives

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Asbestos fibers are highly cytotoxic to cultured mammalian cells and produce chromosomal aberrations in several rodent cell types. There is some uncertainty in the literature as to whether these fibers are clastogenic to cultured human cells. Asbestos fibers do not produce either DNA damage or back mutations in prokaryotic assay systems, and they do not appear to cause DNA strand breaks in either rodent or human cels. The evidence that these fibers can produce either forward mutation or neoplastic transformation of mammalian cells is weak. Asbestos fibers are clearly oncogenic to humans and animals, but, except for clastogenic effects in rodent cells, there is little evidence for genotoxicity of fibers. It is reasonable to expect, therefore, that these materials may be oncogenic by virtue of mechanisms rather than as tumor initiators.

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supporting

confidence: 76%

mentioning

confidence: 85%

mentioning

confidence: 99%

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In Vitro Assessment of Asbestos Genotoxicity

Daniel¹

1983

Environmental Health Perspectives

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“…Other in vitro tests especially designed to detect genotoxicity have, however, generally given more variable results. Positive results in chromosomal aberration studies (Sincock & Seabright, 1975), point mutation (Huang et al, 1978) and sister chromatid exchange analysis (Livingston et al, 1980) have suggested that such bioassays may be suitable for the screening of potentially carcinogenic dusts. Other investigators have, however, reported negative results in the sister chromatid exchange assays (Price-Jones et al, 1980) and bacterial mutation tests (Chamberlain & Tarmy, 1977).…”

Section: Discussionmentioning

confidence: 99%

In vitro genotoxic activities of fibrous erionite

Poole¹,

Brown²,

Turver³

et al. 1983

Br J Cancer

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A high incidence of mesothelioma has been reported from some villages in Cappadocia, Turkey. This type of cancer is usually associated with the inhalation of asbestos, but on the basis of the most prevalent fibre in the dust from these villages, the Turkish outbreak has been attributed to the inhalation of zeolite fibres. A counter hypothesis, based on the detection of very small quantities of chrysotile and tremolite in strata samples and human lung tissue, postulates a significant role of these minerals as one of several factors contributing to pleural disease. A respirable fraction of erionite, (from Oregon, USA, but with similar characteristics to the fibres found in Turkey), has some in vitro genotoxic properties associated with many conventional carcinogens. In this study these fibres caused an increase in morphological transformation and unscheduled DNA repair synthesis (UDS) in C3H10T1/2 cells and UDS in the human lung cell line--A549. It is therefore suggested that exposure to fibrous erionite alone may be sufficient to cause the high incidence of pleural tumours observed in Turkey. Images Figure 1 Figure 3

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“…In vitro studies have shown asbestos to be cytotoxic, clastogenic and, although it is not an Ames assay mutagen, asbestos is mutagenic in systems that detect large deletions of DNA (Both et al, 1994;Hei et al, 1992;Jaurand et al, 1986;Okayasu et al, 1999). Asbestos fibers can induce chromosomal aberrations and large deletion events by interfering with mitotic machinery resulting in the abnormal segregation of chromosomes (Ault et al, 1995;Cole et al, 1991;Dopp et al, 1995;Hesterberg and Barrett, 1985;Jensen et al, 1996;Kodama et al, 1993;Palekar et al, 1987;Sincock and Seabright, 1975;Valerio et al, 1983;Yegles et al, 1993). Workers exposed to asbestos have been reported to have increased levels of sister chromatid exchanges (Fatma et al, 1991;Rom et al, 1983), as do non-smokers with high environmental asbestos exposures (Donmez et al, 1996).…”

Section: Introductionmentioning

confidence: 99%