Induction of Cellulase Biosynthesis by Cellobiose Octaacetate in &lt;i&gt;Aspergillus humicola&lt;/i&gt;

Nipa, Meher Nigad; Sultana, Sharmin; Hakim, MA

doi:10.3329/bjm.v23i2.889

Cited by 5 publications

(4 citation statements)

References 13 publications

(1 reference statement)

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“…An experiment was conducted to find out the effect of different degrees of incubation temperatures ranged from 20 to 35ºC on cellulase production by Alternaria alternata (17) and Aspergillus wentii (10). Results in Table (5) show that the temperatures from 25 to 35ºC achieved high cellulase production in different degrees of incubation .These results are in agreement with Nipa et al (2006) who found that the optimum temperature for CMCase production by the fungus might lie between 27° and 37°C. While Muthuvelayudham and Viruthagiri (2006) reported that the maximum cellulase activity was obtained at 28°C by Trichoderma reesei.…”

Section: Effect Of Incubation Temperaturesupporting

confidence: 88%

Cellulase Production by Two Fungal Strains Isolated From Taif in Saudi Arabia

Nasr

Hussein

Abozaid

et al. 2011

Arab Universities Journal of Agricultural Sciences

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Among 17 fungal isolates isolated from soil of El-hawia, El-hada, El-kaym and Karwa in Taif governorate in Saudi Arabia, two isolates showed high efficacy in producing cellulases enzymes. They were identified to be Alternaria alternata and Aspergillus wentii. Some factors such as carbon and wheat bran as a raw material, nitrogen, pH and incubation temperature were investigated. Results indicated that glucose and cellulose were the most effective as a carbon source while, urea was the best nitrogen source for cellulases production. Initial pH 5.0 and incubation temperatures at 25 or 35ºC achieved high cellulases production. Media used Medium (1): Potato-Dextrose agar (Difco Manual, 1984). It was used for maintenance of fungi .It consists of Potatoe 200.0, Dextrose 20.0, agar 15 g /L, Distilled water 1000.0 ml and pH 5.0.

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Section: Effect Of Incubation Temperaturesupporting

confidence: 88%

Cellulase Production by Two Fungal Strains Isolated From Taif in Saudi Arabia

Nasr

Hussein

Abozaid

et al. 2011

Arab Universities Journal of Agricultural Sciences

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“…Milala et al (2005) also reported maximum cellulase activity after 72 h fermentation by A. niger grown on maize straw and rice husk. On the other hand, Nipa et al (2006) and Akinyele et al (2013) reported 96 h as the optimum fermentation period for cellulase production from A. humicola and A. niger, respectively. However, a short fermentation period for enzyme production offers potential for inexpensive production of enzyme (Sonjoy et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Cellulase Production by Penicillium citrinum using Brewer’s Spent Grain and Pineapple Peels as Cheap, Alternate Substrates

Oyedeji¹,

Opeyemi²

2018

ijSciences

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Cellulases are major group of enzymes with wide-ranging industrial and biotechnological applications. The high cost of cellulase production is a major factor limiting its industrial applications in cellulose bioconversions, hence the need to develop low-cost production systems for this enzyme. Cellulose-rich plant biomass which may be agricultural or industrial in origin exists abundantly as organic wastes which are detrimental to the environment. This study evaluated cellulase production by Penicillium citrinum isolated from deteriorating orange fruits, using brewer's spent grain and pineapple peels as cheap, alternate substrates. Cellulase titres 3.82 ± 0.136 U/mL and 1.405 ± 0.151 U/mL were produced by the fungus, using pineapple peels and brewer's spent grain as substrates, respectively, under submerged fermentation. Maximum cellulase production by P. citrinum occurred with the use of pineapple peels as substrate, after 72 h fermentation period, with the use of pineapple peels at a concentration of 1.5% w / v and peptone as the best nitrogen source. The optima pH and temperature for the production of cellulase by the fungus was found to be 6.0 and 50 o C, respectively. Findings from this study indicated the potential use of pineapple peels as cheaper, alternative substrate for the production of cellulase thus mitigating its hazardous effect on the environment as pollutant. P. citrinum was able to grow and produce good levels of cellulase using solely pineapple peels as low-cost substrate, at high temperature of 50 o C, making this strain and this low-cost agroindustrial residue worthy of further investigation and potentially feasible for a wide range of biotechnological applications.

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“…Cellulases assume greater importance in the current economic scenario due to their versatile industrial and commercial applications [2][3][4]. Though cellulase research has been concentrated mostly in fungi, actinomycetes, and protozoa, there is an increasing interest in cellulose production by bacteria [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Cellulase Production by Pink Pigmented Facultative Methylotrophic Strains (PPFMs)

Shanker

Lalitha

Vadivukkarasi

et al. 2011

Appl Biochem Biotechnol

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Pink pigmented facultative methylotrophs (PPFM) isolated from water samples of Cooum and Adyar rivers in Chennai and soil samples of forests located in various districts of Tamil Nadu, India were screened for cellulase production using carboxymethylcellulose agar (CMC agar) medium. The strains showed wide variations in the production of clearing zones around the colonies on CMC agar medium flooded with Congo red. CMCase and filter paper assays were used to quantitatively measure the cellulase activity of 13 PPFM strains. Among the strains, Methylobacterium gregans, MNW 60, MHW 109, MSF 34, and MSF 40 showed cellulolytic activity ranging from 0.73 to 1.16 U mL(-1) with wide temperature (35-65°C) and pH (5 to 8) tolerance. SDS-PAGE analysis of the crude enzyme of PPFM strain MNW 60 exhibited several protein bands, and zymogram analysis revealed two dimeric cellulase bands with molecular mass of ~92 and 42 kDa. Scanning electron microscopic studies revealed significant morphological differences between the cells grown in normal and CMC amended medium. The strain MNW 60 was identified as Methylobacterium sp. based on biochemical, physiological, and morphological analyses, and the methylotrophic nature was authenticated by the presence of mxaF gene, encoding methanol dehydrogenase as a key indicator enzyme of methylotrophs, with 99% similarity to Methylobacterium lusitanum. With the 16S ribosomal RNA sequence showing 97% similarity to M. lusitanum strain MP2, this can be proposed as a novel taxon of the genus Methylobacterium. The study forms the first detailed report on the extracellular cellulase production by pink pigmented Methylobacterium sp., and it is expected that this might be the basis for further studies on cellulase production by PPFMs to explore the molecular mechanism, strain improvement, and large-scale cellulase production for its application.

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Induction of Cellulase Biosynthesis by Cellobiose Octaacetate in <i>Aspergillus humicola</i>

Cited by 5 publications

References 13 publications

Cellulase Production by Two Fungal Strains Isolated From Taif in Saudi Arabia

Cellulase Production by Two Fungal Strains Isolated From Taif in Saudi Arabia

Cellulase Production by Penicillium citrinum using Brewer’s Spent Grain and Pineapple Peels as Cheap, Alternate Substrates

Cellulase Production by Pink Pigmented Facultative Methylotrophic Strains (PPFMs)

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