Induction of CD8 molecules on thymic γ/δ T cells <i>in vitro</i> is dependent upon α/β T cells

Spetz, Anna-Lena; Kourilsky, Philippe; Larsson-Sciard, Eva-Lotta

doi:10.1002/eji.1830211116

Cited by 7 publications

(3 citation statements)

References 27 publications

(11 reference statements)

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“…This conclusion is based on the observation that: (a) some TCRgd + CD8 À cells may acquire CD8 upon activation [52], (b) that CD2 + CD8a + gd T cells are potentially cytotoxic while other TCRgd + cell subsets are not [53,54] and that (c) CD2 + CD8a + gd T cell are scarce in porcine fetuses [10]. CD8a is expressed on ab and gd T cells following activation, on a subset of NK cells that are directly cytotoxic, on a subset of effector dendritic cells, and on many intra-epithelial lymphocytes from conventional but not from germ-free animals [55][56][57][58]. In swine, in contrast to humans and mice, CD8a and/or MHC-II are not down-regulated so the ab and gd T cell maintain the expression of these entities after activation [33,43,59].…”

Section: Development Of Gd T Cells In the Peripherymentioning

confidence: 99%

The ontogeny of the porcine immune system

Šinkora

Butler

2009

Developmental & Comparative Immunology

145

131

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Cellular and humoral aspects of the immune response develop sequentially in the fetus. During the ontogeny, the pluripotent stem cells emerge and differentiate into all hematopoietic lineages. Basic questions including the identification of the first lympho-hematopoietic sites, the origin of T and B lymphocytes, the development of different subpopulations of alphabeta T, gammadelta T and B lymphocytes as well as development of innate immunity and the acquisition of full immunological capacities are discussed here for swine and compared with other species. The description of related topics such as fertilization, morphogenesis, maternal-fetal-neonatal physiology and early neonatal development are also discussed.

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Section: Development Of Gd T Cells In the Peripherymentioning

confidence: 99%

The ontogeny of the porcine immune system

Šinkora

Butler

2009

Developmental & Comparative Immunology

145

131

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“…IL-2 mainly promotes the proliferation of CD8 + cells ( 48 ). In mice, CD8 expression in γδ T cells seems to occur due to activation with IL-2 and Concanavalin A only in the presence of αβ T cells ( 49 ).…”

Section: Introductionmentioning

confidence: 99%

Chicken γδ T cells proliferate upon IL-2 and IL-12 treatment and show a restricted receptor repertoire in cell culture

Linti,

Göbel,

Früh

2024

Front. Immunol.

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In chickens, γδ T cells represent a large fraction of peripheral T cells; however, their function remains largely unknown. Here, we describe the selective in vitro expansion of γδ T cells from total splenocytes by stimulation with the cytokines IL-2 and IL-12. Under these conditions, γδ T cells proliferated preferentially and reached frequencies of >95% within three weeks. Although IL-2 alone also triggered proliferation, an increased proliferation rate was observed in combination with IL-12. Most of the expanded cells were γδ TCR and CD8 double-positive. Splenocytes sorted into TCR1+CD8+, TCR1highCD8−, and TCR1lowCD8− subsets proliferated well upon dual stimulation with IL-2/IL-12, indicating that none of the three γδ T cell subsets require bystander activation for proliferation. TCR1+CD8+ cells maintained CD8 surface expression during stimulation, whereas CD8− subpopulations showed varied levels of CD8 upregulation, with the highest upregulation observed in the TCR1high subset. Changes in the γδ T-cell receptor repertoire during cell culture from day 0 to day 21 were analyzed by next-generation sequencing of the γδ variable regions. Overall, long-term culture led to a restricted γ and δ chain repertoire, characterized by a reduced number of unique variable region clonotypes, and specific V genes were enriched at day 21. On day 0, the δ chain repertoire was highly diverse, and the predominant clonotypes differed between animals, while the most frequent γ-chain clonotypes were shared between animals. However, on day 21, the most frequent clonotypes in both the γ and δ chain repertoires were different between animals, indicating that selective expansion of dominant clonotypes during stimulation seems to be an individual outcome. In conclusion, IL-2 and IL-12 were sufficient to stimulate the in vitro outgrowth of γδ T cells. Analyses of the TCR repertoire indicate that the culture leads to an expansion of individual T cell clones, which may reflect previous in vivo activation. This system will be instrumental in studying γδ T cell function.

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“…Sie erkennen und zerstören antigentragende Zellen in Abhängigkeit vom MHC-Molekül (Kappes und Tonewaga 1991). TCR-2-positive Zellen sind hingegen phänotypisch entweder CD4 + oder CD8 + (Spetz et al 1991). Die CD4 + werden auch als T-Helferzellen bezeichnet und erkennen Antigene in Verbindung mit Klasse-2-MHC-Molekülen (Gilliland et al 1991, Gay et al 1987.…”

Section: Eine Wesentlicheunclassified

Bedeutung des löslichen CD14-Rezeptors in Plasma und Urin als immunologischer Parameter nach Nierentransplantation und sein Verhältnis zu den löslichen Rezeptoren IL2R, CD4 und CD8

Oliver¹

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Induction of CD8 molecules on thymic γ/δ T cells in vitro is dependent upon α/β T cells

Cited by 7 publications

References 27 publications

The ontogeny of the porcine immune system

The ontogeny of the porcine immune system

Chicken γδ T cells proliferate upon IL-2 and IL-12 treatment and show a restricted receptor repertoire in cell culture

Bedeutung des löslichen CD14-Rezeptors in Plasma und Urin als immunologischer Parameter nach Nierentransplantation und sein Verhältnis zu den löslichen Rezeptoren IL2R, CD4 und CD8

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