Induction of carotenogenesis in cultured cells of Lycopersicon esculentum

Fosket, Donald E.; Radin, David N.

doi:10.1016/0304-4211(83)90215-8

Cited by 29 publications

(13 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bleaching herbicides, utilized to enhance in vitro carotenogenesis (56,(92)(93)(94)(95), are chemicals that interrupt the normal biosynthesis of carotenoids, either in vivo or in vitro, causing a fatal loss of photoprotection in the plant and leading to the destruction of chlorophyll upon light exposure (96). Previously, our laboratory utilized the bleaching herbicide norflurazon, a PE desaturase inhibitor (Fig.…”

Section: Isotopically Labeled Tomato Carotenoid Tracersmentioning

confidence: 99%

Nutritional Aspects of Phytoene and Phytofluene, Carotenoid Precursors to Lycopene

Engelmann

Clinton

Erdman

2011

Advances in Nutrition

View full text Add to dashboard Cite

Epidemiological studies suggest an inverse relationship between tomato consumption and serum and tissue lycopene (LYC) levels with risk of some chronic diseases, including several cancers and cardiovascular disease. LYC, the red carotenoid found in tomatoes, is often considered to be the primary bioactive carotenoid in tomatoes that mediates health benefits, but other colorless precursor carotenoids, phytoene (PE) and phytofluene (PF), are also present in substantial quantities. PE and PF are readily absorbed from tomato foods and tomato extracts by humans. Animal models of carotenoid absorption suggest preferential accumulation of PE and PF in some tissues. The reasonably high concentrations of PE and PF detected in serum and tissues relative to the concentrations in foods suggest that absorption or metabolism of these compounds may be different from that of LYC. Experimental studies, both in vitro and in vivo, suggest that PE and PF exhibit bioactivity but little is known about their impact in humans. Methods for producing isotopically labeled PE, PF, and LYC tracers from tomato plant cell culture offer a unique tool for further understanding the differential bioavailability and metabolism of these 3 prominent tomato carotenoids and how they may affect health.

show abstract

Section: Isotopically Labeled Tomato Carotenoid Tracersmentioning

confidence: 99%

Nutritional Aspects of Phytoene and Phytofluene, Carotenoid Precursors to Lycopene

Engelmann

Clinton

Erdman

2011

Advances in Nutrition

View full text Add to dashboard Cite

show abstract

“…Bleaching herbicides increase total carotenoid content in tomato, pepper, and daffodil plants as well as tomato cell cultures (14, 16, 17, 19, 20, 24–26). The results from the current study confirmed either or both herbicides led to a greater total carotenoid yield than untreated cultures (135–193 fold).…”

Section: Discussionmentioning

confidence: 99%

Herbicide Treatments Alter Carotenoid Profiles for ¹⁴C Tracer Production from Tomato (Solanum lycopersicum cv. VFNT cherry) Cell Cultures

Engelmann

Rogers

Lila

et al. 2009

J. Agric. Food Chem.

View full text Add to dashboard Cite

Progress in learning about underlying carotenoid bioactivity mechanisms has been limited due to lack of commercially available radiolabeled lycopene (LYC), phytoene (PE), and phytofluene (PF). Tomato (Solanum lycopersicum cv. VFNT cherry) cell cultures have been treated to produce [ 14 C]-PE and PF but with relatively low yields. To increase carotenoid production, two bleaching herbicides were administered during the culture incubation, 2-(4-chlorophenyl-thio) triethylamine and norflurazon separately or in combination to produce varying ratios of PE, PF, and LYC. Treatment with both herbicides resulted in optimal production of all three carotenoids. Subsequently, cultures were incubated in [ 14 C] sucrose-containing media to produce labeled LYC, PE, and PF. Adding [ 14 C] sucrose on day 1 of the 14-d culture incubation cycle to norflurazon-treated cultures led to a small increase in labeling efficiency compared to adding it on day 7. Improved culture conditions efficiently provided sufficient 14 C-carotenoids for future cell culture and animal metabolic tracking studies.

show abstract

“…and from C~~sjcz~~~l chromoplasts 1391. Suggestions have been made that CPTA and related compounds act at the level of gene expression rather than by directly affecting enzyme function [37,40,413. More recent work, using a cell-free system from the cyanobacterium &.&anocupsa.…”

Section: Discussionmentioning

confidence: 99%

Cloning and functional expression in Escherichia coli of a cyanobacterial gene for lycopene cyclase, the enzyme that catalyzes the biosynthesis of β‐carotene

et al. 1993

View full text Add to dashboard Cite

Carotenoids with cyclic end groups are essential components of the photosynthetic membrane in all known oxygenic photosynthetic organisms. These yellow pigments serve the vital role of protecting against potentially lethal photo‐oxidative damage. Many of the enzymes and genes of the carotenoid biosynthetic pathway in cyanobacteria, algae and plants remain to be isolated or identified. We have cloned a cyanobacterial gene encoding lycopene cyclase, an enzyme that converts the acyclic carotenoid lycopene to the bicyclic molecule β‐carotene. The gene was identified through the use of an experimental herbicide, 2‐(4‐methylphenoxy)triethylamine hydrochloride (MPTA), that prevents the cyclization of lycopene in plants and cyanobacteria. Chemically‐induced mutants of the cyanobacterium Synechococcus sp. PCC7942 were selected for resistance to MPTA, and a mutation responsible for this resistance was mapped to a genomic DNA region of 200 bp by genetic complementation of the resistance in wild‐type cells. A 1.5 kb genomic DNA fragment containing this MPTA‐resistance mutation was expressed in a lycopene‐accumulating strain of Escherichia coli. The conversion of lycopene to β‐carotene in these cells demonstrated that this fragment encodes the enzyme lycopene cyclase. The results indicate that a single gene product, designated lcy, catalyzes both of the cyclization reactions that are required to produceβ‐carotene from lycopene, and prove that this enzyme is a target site of the herbicide MPTA. The cloned cyanobacterial lcy gene hybridized well with genomic DNA from eukaryotic algae, thus it will enable the identification and cloning of homologous genes for lycopene cyclase in algae and plants.

show abstract

Induction of carotenogenesis in cultured cells of Lycopersicon esculentum

Cited by 29 publications

References 23 publications

Nutritional Aspects of Phytoene and Phytofluene, Carotenoid Precursors to Lycopene

Nutritional Aspects of Phytoene and Phytofluene, Carotenoid Precursors to Lycopene

Herbicide Treatments Alter Carotenoid Profiles for ¹⁴C Tracer Production from Tomato (Solanum lycopersicum cv. VFNT cherry) Cell Cultures

Cloning and functional expression in Escherichia coli of a cyanobacterial gene for lycopene cyclase, the enzyme that catalyzes the biosynthesis of β‐carotene

Contact Info

Product

Resources

About

Induction of carotenogenesis in cultured cells of Lycopersicon esculentum

Cited by 29 publications

References 23 publications

Nutritional Aspects of Phytoene and Phytofluene, Carotenoid Precursors to Lycopene

Nutritional Aspects of Phytoene and Phytofluene, Carotenoid Precursors to Lycopene

Herbicide Treatments Alter Carotenoid Profiles for 14C Tracer Production from Tomato (Solanum lycopersicum cv. VFNT cherry) Cell Cultures

Cloning and functional expression in Escherichia coli of a cyanobacterial gene for lycopene cyclase, the enzyme that catalyzes the biosynthesis of β‐carotene

Contact Info

Product

Resources

About

Herbicide Treatments Alter Carotenoid Profiles for ¹⁴C Tracer Production from Tomato (Solanum lycopersicum cv. VFNT cherry) Cell Cultures