2002
DOI: 10.1096/fj.02-0096fje
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Induction of cancer cell migration by epidermal growth factor is initiated by specific phosphorylation of tyrosine 1248 of c‐erbB‐2 receptor via epidermal growth factor receptor

Abstract: Induction of tumor cell migration is a key step in invasion and metastasis. Here we report that the epidermal growth factor (EGF)-induced cell migration of breast cancer cells is attributed to a transient, rather than a sustained, activation of phospholipase C (PLC)-gamma1 due to c-erbB-2 signaling. EGF stimulation of EGF receptor (EGFR) overexpressing cells resulted in long-term PLC-gamma1 tyrosine phosphorylation and sustained levels of inositol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG) producing sin… Show more

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Cited by 127 publications
(141 citation statements)
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“…The breast adenoma carcinoma cell line MDA-MB-468-HER2 (MDA-HER2; EGFR and c-erbB-2 positive) (Dittmar et al, 2002) was maintained in Leibowitz L-15 media (PAA, Linz, Austria) supplemented with 10% heat-inactivated foetal calf serum (PAA), 10 U ml À1 penicillin, 10 mg ml À1 streptomycin and 400 mg ml À1 G418 (Merck BioSciences GmbH, Schwalbach, Germany) in 371C humidified atmosphere without CO 2 supplementation.…”
Section: Cells and Culture Conditionsmentioning
confidence: 99%
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“…The breast adenoma carcinoma cell line MDA-MB-468-HER2 (MDA-HER2; EGFR and c-erbB-2 positive) (Dittmar et al, 2002) was maintained in Leibowitz L-15 media (PAA, Linz, Austria) supplemented with 10% heat-inactivated foetal calf serum (PAA), 10 U ml À1 penicillin, 10 mg ml À1 streptomycin and 400 mg ml À1 G418 (Merck BioSciences GmbH, Schwalbach, Germany) in 371C humidified atmosphere without CO 2 supplementation.…”
Section: Cells and Culture Conditionsmentioning
confidence: 99%
“…The analysis of PLC-g1 tyrosine phosphorylation by Western Blot was performed as previously described (Dittmar et al, 2002) with minor modifications. MDA-HER2 cells (4 Â 10 6 ) were pretreated with PS1/2-TAT (133 mM, 30 min) prior to EGF treatment (100 ng ml À1 , 2 min).…”
Section: Immunoprecipitation and Western Blotmentioning
confidence: 99%
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