Induction of Cancer Cell Death by Isoflavone: The Role of Multiple Signaling Pathways

Li, Yiwei; Kong, Dejuan; Bao, Bin; Ahmad, Aamir; Sarkar, Fazlul H.

doi:10.3390/nu3100877

Cited by 52 publications

(53 citation statements)

References 122 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Isoflavones act as selective estrogen receptor modulators, exhibiting agonist or antagonist activity depending on local estrogen concentrations (Fritz et al 2013). They are also reported to affect multiple cellular signalling pathways, protect cells from oxidative damage and inhibit angiogenesis (Li et al 2011;Mahmoud et al 2014). Numerous clinical trials indicate that regular consumption of isoflavone-rich foods may reduce the risk of breast and prostate cancers (Fritz et al 2013;Mourouti and Panagiotakos 2013;van Die et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Plant growth regulators affect biosynthesis and accumulation profile of isoflavone phytoestrogens in high-productive in vitro cultures of Genista tinctoria

Łuczkiewicz

Kokotkiewicz

Głód

2014

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The influence of plant growth regulators on biomass growth and the accumulation of medicinally-relevant isoflavone phytoestrogens, derivatives of genistein and daidzein (8 compounds including aglycones, glucosides and glucoside esters) in callus cultures of Genista tinctoria (Fabaceae) was examined. The experiments included 10 auxins [2,4-dichlorophenoxyacetic acid (2,4-D), p-chlorophenoxyacetic acid, indole-3-acetic acid, indole-3-butyric acid, indole-3-propionic acid, 1-naphthaleneacetic acid, b-naphthoxyacetic acid, picloram, 2,3,5-triiodobenzoic acid (TIBA), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T)] and 7 cytokinins [6-benzylaminopurine, forchlorfenuron, 1,3-diphenylurea, 2-isopentenyladenine, kinetin (KIN), thidiazuron, zeatin] applied at 0.5 and 5.0 mg l Among the cytokinins tested, the highest isoflavone content (6,436.26 mg/100 g dry weight) and the fastest biomass growth (Gi = 892.46 %) were obtained for 0.5 mg l -1 KIN used jointly with 5.0 mg l -1 2,4-D. In the group of auxins, the combination of 0.5 mg l -1 TIBA and 5.0 mg l -1 KIN provided the fastest culture growth (Gi = 983.07 %) and the isoflavone concentration of 10,474.23 mg/100 g dry weight, which is so far the highest amount of these metabolites achieved in callus cultures of higher plants.

show abstract

Section: Introductionmentioning

confidence: 99%

Plant growth regulators affect biosynthesis and accumulation profile of isoflavone phytoestrogens in high-productive in vitro cultures of Genista tinctoria

Łuczkiewicz

Kokotkiewicz

Głód

2014

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Increase in tumor invasion and activation of alternative angiogenic pathways are the reported escape mechanisms adopted by tumor cells [14,15]. Thus, the current focus of research has shifted to either designing combination therapies or exploring small molecules for their multitargeting properties [24,28,29].…”

Section: Discussionmentioning

confidence: 99%

“…Small molecules such as biochanin A have become popular in recent years as treatment options for different types of cancers. They prove beneficial because of their multitargeting properties and low toxicity [24]. Because biochanin A is less toxic than genistein and its antiangiogenic properties have not been elucidated, this study examines the effects of biochanin A on endothelial cell functions and proangiogenic mechanisms using C6 rat glioma cells, murine bEnd.3 brain endothelial cells, and an ex-vivo chick chorioallantoic membrane (CAM) model.…”

Section: Introductionmentioning

confidence: 99%

Biochanin A inhibits endothelial cell functions and proangiogenic pathways

2015

View full text Add to dashboard Cite

Malignant gliomas, such as glioblastoma multiforme, are highly vascularized tumors of the central nervous system. A rich network of angiogenic vessels supporting glioma growth is an important therapeutic target in glioma therapy. In the past few years, small molecules have gained interest as multitargeting therapies for cancer. Biochanin A is a small, natural dietary isoflavone known for its anticancer potential. Previously, we have found that biochanin A inhibits invasion in human glioblastoma cells. In this study, we elucidated the antiangiogenic mechanisms of biochanin A using rat brain tumor (C6) and murine brain endothelial (bEnd.3) cells and an ex-vivo chick chorioallantoic membrane model. Biochanin A inhibited endothelial cell functions such as cell viability, migration, and invasion, as analyzed using MTT, scratch wound, and gelatin zymography assays. Activation of proangiogenic proteins (ERK/AKT/mTOR) was inhibited. Biochanin A also inhibited chemical hypoxia-inducible factor-1α and vascular endothelial growth factor in C6 cells. Results of chick chorioallantoic membrane assay showed that biochanin A inhibited blood vessel formation ex vivo. As these results suggest that biochanin A directly targets different facets of angiogenesis in vitro and ex vivo, this study provides a rationale for future preclinical evaluation of its efficacy against angiogenic gliomas.

show abstract

“…Genistein, daidzein, and glycitein are the three main isoflavones found in soybeans. Isoflavone genistein shows anti-oxidant and anti-cancer activities (11). Biochanin A is another isoflavone found in red clover or soy and is known to exert its anti-cancer effect on various cancers (12).…”

Section: Introductionmentioning

confidence: 99%

The Role of Nutraceuticals in Pancreatic Cancer Prevention and Therapy

Li¹,

Go²,

Sarkar³

2015

Pancreas

Self Cite

View full text Add to dashboard Cite

Pancreatic cancer is one of the most aggressive malignancies in US adults. The experimental studies have found that antioxidant nutrients could reduce oxidative DNA damage, suggesting that these antioxidants may protect against pancreatic carcinogenesis. Several epidemiologic studies showed that dietary intake of antioxidants was inversely associated with the risk of pancreatic cancer, demonstrating the inhibitory effects of antioxidants on pancreatic carcinogenesis. Moreover, nutraceuticals, the anti-cancer agents from diet or natural plants, have been found to inhibit the development and progression of pancreatic cancer through the regulation of cellular signaling pathways. Importantly, nutraceuticals also up-regulate the expression of tumor suppressive miRNAs and down-regulate the expression of oncogenic miRNAs, leading to the inhibition of pancreatic cancer cell growth and pancreatic Cancer Stem Cell (CSC) self-renewal through modulation of cellular signaling network. Furthermore, nutraceuticals also regulate epigenetically deregulated DNAs and miRNAs, leading to the normalization of altered cellular signaling in pancreatic cancer cells. Therefore, nutraceuticals could have much broader use in the prevention and/or treatment of pancreatic cancer in combination with conventional chemotherapeutics. However, more in vitro mechanistic experiments, in vivo animal studies, and clinical trials are needed to realize the true value of nutraceuticals in the prevention and/or treatment of pancreatic cancer.

show abstract

Induction of Cancer Cell Death by Isoflavone: The Role of Multiple Signaling Pathways

Cited by 52 publications

References 122 publications

Plant growth regulators affect biosynthesis and accumulation profile of isoflavone phytoestrogens in high-productive in vitro cultures of Genista tinctoria

Plant growth regulators affect biosynthesis and accumulation profile of isoflavone phytoestrogens in high-productive in vitro cultures of Genista tinctoria

Biochanin A inhibits endothelial cell functions and proangiogenic pathways

The Role of Nutraceuticals in Pancreatic Cancer Prevention and Therapy

Contact Info

Product

Resources

About