Induction of calcite precipitation through heightened production of extracellular carbonic anhydrase by CO2 sequestering bacteria

Sundaram, Smita; Thakur, Indu Shekhar

doi:10.1016/j.biortech.2018.01.081

Cited by 35 publications

(16 citation statements)

References 15 publications

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“…Interestingly, the nature of the compound obtained was similar in solid and liquid cultures for most isolated marine bacteria. Moreover, the fact that the marine bacterial strains induced CaCO 3 precipitation both in presence and absence of urea suggests that several metabolic pathways could be involved in biocalcification, including the urease pathway in presence of urea [12] and anhydrase carbonic pathway in absence of urea [28]. Therefore, we explored whether the bacteria expressed the enzymes involved in these two metabolisms.…”

Section: Identification Of Caco 3 -Precipitating Bacteria and Charactmentioning

confidence: 99%

“…Carbonic anhydrase is a major enzyme that plays an important role in carbon concentrating mechanism and sequestration of CO 2 into calcium carbonate [29]. It is reported that an extracellular Bacillus carbonic anhydrase produced calcite [28]. This suggests that the carbonic anhydrase pathway alone can induce biocalcification and this may explain the formation of CaCO 3 in urease-negative strains and urease-positive strains without urea in the medium.…”

Section: Metabolic Pathways Involved In Biomineralizationmentioning

confidence: 99%

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Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits

Vincent

Sabot

Lanneluc

et al. 2020

Matériaux & Techniques

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Biomineralization induced by microbial enzymes, which catalyse CaCO3 precipitation, is a promising field of research for various applications in building eco-materials. Especially, this could provide an eco-friendly process for protection of coastal areas against erosion. In the present investigation, fourteen bacterial strains were isolated and characterized from both natural seawater and calcareous deposits formed on a cathodically protected steel mesh in marine environment. All of them induced calcium carbonate precipitation in various media by producing urease and/or carbonic anhydrase enzymes. The calcium carbonate minerals produced by bacteria were identified by microscopy and µ-Raman spectroscopy. In parallel, an experimental set-up, based on a column reactor, was developed to study biomineralization and microbial capacity of Sporosarcina pasteurii to form sandy agglomerate. These well-known calcifying bacteria degraded the urea present in liquid medium circulating through the column to produce calcium carbonate, which acted as cement between sand particles. The bio-bricks obtained after 3 weeks had a compressive strength of 4.2 MPa. 20% of the inter-granular voids were filled by calcite and corresponded to 13% of the total mass. We successfully showed that bio-column system can be used to evaluate the bacterial ability to agglomerate a sandy matrix with CaCO3.

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Section: Identification Of Caco 3 -Precipitating Bacteria and Charactmentioning

confidence: 99%

Section: Metabolic Pathways Involved In Biomineralizationmentioning

confidence: 99%

Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits

Vincent

Sabot

Lanneluc

et al. 2020

Matériaux & Techniques

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“…Liu et al [109] have reported that CA can be used to accelerate an aqueous processing route to carbonate formation. Sundaram et al [110] have proved that calcite formation is successfully obtained by using Bacillus sp. with enhanced activity of CA, a much higher rate of CaCO3 precipitation can be observed with increased CA activity.…”

Section: Ph Increasementioning

confidence: 99%

The Significant Role of Different Magnesium: Carbonate Minerals Induced by Moderate Halophile <em>Staphylococcus epidermis</em> Y2

Han¹,

Yu²,

Zhao³

et al. 2018

Preprint

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Carbonate precipitation induced by microorganism has become a hot spot in the field of carbonate sedimentology, while the effect of different magnesium on biominerals has rarely been studied. Therefore, magnesium sulfate and magnesium chloride were used to investigate the significant role played on carbonate minerals. In this study, Staphylococcus epidermidis Y2 was isolated and identified by 16S rDNA homology comparison. The ammonia, pH, carbonic anhydrase, carbonate and bicarbonate ions were investigated. The mineral phase, morphology and elemental composition were analyzed by XRD and SEM-EDS. The ultrathin slices of bacteria were analyzed by HRTEM-SAED and STEM. The result showed that this bacterium could release ammonia and carbonic anhydrase to increase pH, and elevate the supersaturation via a large number of carbonate and bicarbonate ions released through carbon dioxide hydration catalyzed by carbonic anhydrase. The crystal cell density of monohydrocalcite was lower in magnesium chloride medium than that in magnesium sulfate medium. The crystal grew in a mode of spiral staircas in magnesium sulfate medium, while in a concentric circular pattern in magnesium chloride medium. There was no obvious intracellular biomineralization. This study may be helpful to further understand the biomineralization mechanism, may also provide some references for the reconstruction of paleogeological environment.

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“…6 (b)) showed that the optimum temperature for CA activity was 35°C after that decline in CA activity was observed. In the previous study, the optimum temperature for purified CA was found to be 60°C [36]. Mostly, CA isolated from human erythrocyte and bovine shows CA activity in the temperature range of 35-40°C and in the pH range of 6.5-7.5 [39].…”

Section: Optimization Of Reaction Parameters For Camentioning

confidence: 95%

“…In another study, CA from Bacillus sp. gave maximum activity at pH 8 whereas CA from Lactobacillus showed optimum activity at pH 6 [36,37]. However, CA from Helicobacter pylori showed optimum activity in an acidic environment and high acid tolerance [38].…”

Section: Optimization Of Reaction Parameters For Camentioning

confidence: 99%

Efficient reduction of CO2 using a novel carbonic anhydrase producing Corynebacterium flavescens

Sharma

Kumar

2020

Environmental Engineering Research

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Emission of greenhouse gases into the atmosphere by human activities leads to global warming. To reduce the level of CO2 the bio-catalytic properties of microbial carbonic anhydrase (CA) can be exploited. The present study aimed to isolate CA producing bacteria from cow saliva. After thorough screening for CA activity in the bacterial cultures, ten isolates were selected. Out of ten bacterial isolates, T5 isolate showed the highest CA activity (83.92 U/mL) and the isolate was identified as Corynebacterium flavescens using 16s rRNA analysis. Various production parameters for the optimum production of CA were optimized. During the optimization, incubation temperature 40°C, agitation speed 120 rpm, and inoculum volume 4%v/v was found to be optimum for CA production. The optimum reaction pH, reaction time and temperature were 7, 10 min and 35°C, respectively. The crude enzyme was tested for the conversion of CO2 into the calcium carbonate (CaCO3) under controlled conditions. The CO2 conversion efficacy of crude CA was observed to be ~45 mg CaCO3/mg protein. The synthesized CaCO3 was analyzed using scanning electron microscopy and X-ray diffraction techniques for particle size, morphology and elemental structure. Calcite precipitation by bacterial CA makes it a potential candidate to be effectively employed in biomimetic CO2 sequestration.

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Induction of calcite precipitation through heightened production of extracellular carbonic anhydrase by CO2 sequestering bacteria

Cited by 35 publications

References 15 publications

Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits

Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits

The Significant Role of Different Magnesium: Carbonate Minerals Induced by Moderate Halophile <em>Staphylococcus epidermis</em> Y2

Efficient reduction of CO2 using a novel carbonic anhydrase producing Corynebacterium flavescens

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