Induction of c-Jun by air particulate matter (PM10) of Mexico city: Participation of polycyclic aromatic hydrocarbons

Salcido-Neyoy, Martha Estela; Sánchez-Pérez, Yesennia; Osornio-Vargas, Álvaro; Gonsebatt, María E.; Meléndez-Zajgla, Jorge; Morales‐Bárcenas, Rocío; Petrosyan, Pavel; Molina-Servin, Edith Danny; Vega, Elizabeth; Manzano-León, Natalia; García-Cuéllar, Claudia M.

doi:10.1016/j.envpol.2015.03.051

Cited by 28 publications

(15 citation statements)

References 63 publications

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“…It is a model that reflects what could be occurring in other cell types. The presence of genotoxic agents such as metals and polycyclic aromatic hydrocarbons have been determined in the PM of MCMA (Garcia-Suastegui et al, 2011;Salcido-Neyoy et al, 2015). Fine PM such as PM2.5 can penetrate tissues and cells (WHO, 2013).…”

Section: Discussionmentioning

confidence: 99%

Particulate matter‐associated micronuclei frequencies in maternal and cord blood lymphocytes

Sordo

Maciel‐Ruiz

Salazar

et al. 2019

Environ and Mol Mutagen

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Studies associate particulate matter (PM) exposure with pulmonary, cardiovascular, and neurologic diseases. Elevated levels of coarse (PM10) and fine (PM2.5) PM have been reported in the Mexico City metropolitan area during the last two decades. There is limited information if these conditions affect newborns. We associated maternal exposure to PM reported by the monitoring stations considering the place of residence of each participant with the presence of genotoxic damage (cytome analysis) in maternal and umbilical cord blood (UCB) lymphocytes. Eighty‐four healthy women in their last quarter of pregnancy met the inclusion criteria. Each volunteer exposure was estimated according to the average PM2.5 and PM10 levels during the last month of gestation. The micronuclei (MN) frequencies in UCB lymphocyte cultures ranged between 0 and 9. They also showed lower cell proliferation indexes than their mothers. There was a strong correlation between the maternal and the UCB MN frequency (ρ = 0.3767, P = 0.0002). Multiple regression analysis including PM10 and PM2.5 levels, maternal age, and occupation, showed a significant and positive association between UCB MN frequency and PM2.5. A statistically significant increase in the MN frequency in both maternal and UCB lymphocytes was observed in samples obtained during the dry season (higher PM levels) as compared with the MN frequency in blood samples obtained during the rainy season (lower PM levels). These results suggest that PM, mainly PM2.5, can cross the placenta causing DNA damage in fetal cells which may increase the potential for diseases during childhood or adult life. Environ. Mol. Mutagen. 60:421–427, 2019. © 2019 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Particulate matter‐associated micronuclei frequencies in maternal and cord blood lymphocytes

Sordo

Maciel‐Ruiz

Salazar

et al. 2019

Environ and Mol Mutagen

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“…Impact of the particulate matter and organic extracts were also investigated by other authors. Among many results of researches it was measured apoptosis an viability of cells and cytotoxic effects using different parameters tests [11,[14][15][16].…”

Section: Discussionmentioning

confidence: 99%

“…The choice of adherent A549 cells allows to stimulate conditions as close as in real ingress finest fractions of particulate matter pollutions to the alveoli. A549 cell line is widely used as an in vitro model for various studies, including cytotoxicity studies of air pollutions [7,11].…”

Section: A549 Cell Linementioning

confidence: 99%

Cytotoxicity properties of PM2.5 collected in Wrocław agglomeration

Bełcik

Piekarska

2017

E3S Web Conf.

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Abstract. WHO report published in May 2016 presents the cities with the highest concentrations of particulate matter fraction 10 μm and 2.5 μm on the earth. Report presents that many of Polish cities are on the top of polluted regions in European Union. One of the cities mentioned in the report is Wrocław. Wrocław is the urban and industrial agglomeration, where air pollutions comes from three main sources: low-emissions, industrial emissions and urban traffic. In recent years, reduction in annual average concentrations of particulate matter has been observed. However Wrocław is still one of the cities in Poland, which have problems with smog situations during winter season. This article presents results of researches conducted on the particulate matter fraction 2.5 μm collected in Wrocław agglomeration. Pollutions have been tested using PAN-I assay kit, which contains 4 single toxicity tests allows to check different parameters defining toxic effects on human cells. Cytotoxicity tests which are conducted are: LDH test to evaluate the integrity of cell membrane, XTT test to assess mitochondrial activity, NR test for the evaluation of the lysosomal activity and SRB test to evaluate the total protein content. Tests were performed on adenocarcinomic human alveolar basal epithelial cell line (A549), which allows to simulate conditions as close as real.

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“…Among many results of researches it was measured apoptosis an viability of A549 cell line and cytotoxic effects using different parameters including LDH test, MTT test, which is alternative to the XTT, and MDH test [10,[15][16][17].…”

Section: Pan-i Cytotoxicity Testmentioning

confidence: 99%

Section: A549 Cell Linementioning

confidence: 99%

Cytotoxicity and genotoxicity properties of particulate matter fraction 2.5 μm

et al. 2017

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Abstract. In the ambient is more than 2,000 chemical substances, some of them are absorbed on the surface of the particulate matter and may causes many health problems. Air pollution is responsible for more than 3.2 million premature deaths which classifies it as a second place environmental risk factor. Especially dangerous for health are polycyclic aromatic hydrocarbons and their nitro-and amino derivatives which shows mutagenic and carcinogenic properties. Air pollutions were also classified by International Agency for Research on Cancer to group which carcinogenic properties on human were proved by available knowledge. Air pollutions, including particulate matter are one of the biggest problem in Polish cities. World Health Organization in report published in May 2016 set many of Polish cities on the top of the list most polluted in European Union. The article presents results of mutagenicity, genotoxicity and cytotoxicity researches conducted on a particulate matter fraction 2.5 µm collected during all year long in Wroclaw agglomeration. The material were collected on filters using high-flow air aspirator and extracted using dichloromethane. Additionally it was fractionated into 2 parts containing: all pollutants and only polycyclic aromatic hydrocarbons. Dry residue of this fractions were dissolving in DMSO and tested using biological methods. Biological methods include mutagenicity properties which are investigated by Salmonella assay (Ames assay). Other biological method was comet assay and 4 parameter cytotoxicity test PAN-I assay. Results of the conducted experiments shows differences in mutagenic, genotoxic and cytotoxic properties between seasons of collection and between volume of dust pollutions fractions. The worst properties shows particles collected in autumn and winter season and this containing only polycyclic aromatics hydrocarbons. Results showed also some correlations in results obtained during different methods and properties.

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Induction of c-Jun by air particulate matter (PM10) of Mexico city: Participation of polycyclic aromatic hydrocarbons

Cited by 28 publications

References 63 publications

Particulate matter‐associated micronuclei frequencies in maternal and cord blood lymphocytes

Particulate matter‐associated micronuclei frequencies in maternal and cord blood lymphocytes

Cytotoxicity properties of PM2.5 collected in Wrocław agglomeration

Cytotoxicity and genotoxicity properties of particulate matter fraction 2.5 μm

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