Induction of Blastocyst Implantation in Mice by Cyclic Amp

Holmes, Paul; Bergström, Staffan

doi:10.1530/jrf.0.0430329

Cited by 31 publications

(11 citation statements)

References 7 publications

(7 reference statements)

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“…In contrast, increased levels of cAMP resulting from the activation of EP 2 or EP 4 may participate in the process of implantation and decidualization. Indeed, previous reports [36][37][38][39][40][41], as well as the present observation of luminal epithelial and stromal cell expression of EP 4 on Days 4 and 5, suggest the involvement of PGE 2 and cAMP in these processes. Since cAMP can exert opposite effects on cell proliferation depending on the cell types involved [42], differential expression of EP 3 and EP 4 in the decidualizing stroma on Days 5-8 sug- gests that PGE 2 effects on decidualization (proliferation and differentiation) should involve these receptor subtypes.…”

Section: Discussionsupporting

confidence: 84%

Potential Sites of Prostaglandin Actions in the Periimplantation Mouse Uterus: Differential Expression and Regulation of Prostaglandin Receptor Genes1

Yang

Das

Wang

et al. 1997

Biology of Reproduction

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Prostaglandins (PGs), especially PGE2 and PGF2alpha, are considered important for blastocyst spacing, implantation, and decidualization in the rodent uterus. However, information regarding uterine sites of PG actions in these processes is lacking. PGE2 or PGF2alpha interacts with specific G protein-coupled membrane receptors. PGE2 receptors are classified into four subtypes, EP1, EP2, EP3, and EP4. While EP1 is coupled to Ca2+ mobilization, activation of EP2 and EP4 triggers stimulation of adenylyl cyclase. In contrast, activation of EP3 inhibits adenylyl cyclase. PGF2alpha receptor (FP) is coupled to stimulation of phospholipase C-inositol trisphosphate (IP3) pathway and Ca2+ mobilization. This investigation demonstrates that PGE2 and PGF2alpha receptor genes are expressed in a temporal and cell-specific manner in the periimplantation mouse uterus. In the mouse, the attachment reaction occurs in the evening (2200-2300 h) of Day 4 of pregnancy and is preceded by embryo spacing, uterine edema, and luminal closure resulting in an intimate apposition of the blastocyst with the uterine luminal epithelium. Expression of EP3 and FP primarily in the circular muscle of the myometrium on Days 3-5 of pregnancy suggests that the circular muscle, not the longitudinal muscle, is the primary target for PG-mediated uterine contractions required for embryo transport, spacing, and/or accommodation in the uterus. In contrast, expression of EP3 in a subpopulation of cells in the stromal bed at the mesometrial side, and of EP4 in the epithelium and stroma on these days, suggests that PGE2 effects on uterine preparation for implantation (such as epithelial cell differentiation, stromal cell proliferation, uterine edema, luminal closure, and increased localized endometrial vascular permeability at the sites of blastocyst attachment) are mediated by these receptor subtypes. Similar expression patterns of EP3 and EP4 in the Day 4 pseudopregnant mouse uterus or in the ovariectomized uterus under combined treatment with estrogen and progesterone suggest that these genes are regulated by ovarian steroids rather than by the embryo during the preimplantation period (Days 1-4). In contrast, the expression of these genes during the postimplantation period (Days 5-8) is associated with the onset of decidualization.

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Section: Discussionsupporting

confidence: 84%

Potential Sites of Prostaglandin Actions in the Periimplantation Mouse Uterus: Differential Expression and Regulation of Prostaglandin Receptor Genes1

Yang

Das

Wang

et al. 1997

Biology of Reproduction

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“…This suggests that basic metabolic factors, in addition to transcriptional and cell signaling pathways, can be important determinants of successful early embryonic development. For example, following blastocyst activation in mice, there is a shift in metabolic substrates from pyruvate to glucose (116,117). Along these lines, there is a substantial increase in facilitative glucose transporter expression between fertilization and blastocyst formation (118); inactivation of the genes encoding glucose transporter 3 (GLUT3) or GLUT8 results in blastocyst incompetence (119)(120)(121)(122).…”

Section: Metabolic Regulation Of Placental and Early Embryonic Develomentioning

confidence: 99%

The placenta: transcriptional, epigenetic, and physiological integration during development

Maltepe¹,

Bakardjiev²,

Fisher³

2010

J. Clin. Invest.

251

204

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The placenta provides critical transport functions between the maternal and fetal circulations during intrauterine development. Formation of this interface relies on coordinated interactions among transcriptional, epigenetic, and environmental factors. Here we describe these mechanisms in the context of the differentiation of placental cells (trophoblasts) and synthesize current knowledge about how they interact to generate a functional placenta. Developing an understanding of these pathways contributes to an improvement of our models for studying trophoblast biology and sheds light on the etiology of pregnancy complications and the in utero programming of adult diseases.

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“…Recently, two groups of workers have succeeded in inducing implantation by the intraluminal injection of cyclic AMP (Webb, 1975;Holmes and Bergstrom, 1975). This is an area in which advances can be expected in the near future.…”

Section: Mode Of Action Of Luteal-phase Estrogen In Inducing Endometrmentioning

confidence: 99%

The Implantation Reaction

Finn

1977

Biology of the Uterus

171

121

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Implantation involves the attachment of the blastocyst to the wall of the uterus with, in some cases, the embedding of the entire ovum in the uterine wall. Associated with it are very profound and characteristic changes in the endometrium both during preparation for implantation and following attachment of the blastocyst. In this chapter, the uterine changes that take place during preparation will be considered first and then the changes during the process of implantation. The former occur regularly and frequently throughout the life of most female mammals, whereas the latter are rarer events, especially in women. This division of the subject is also convenient for the consideration of the control mechanism involved. Whereas the preparative changes are largely directed by the varying levels of the hormones secreted by the ovary, once the implantation process has been initiated hormones playa largely permissive role.There is considerable species variation in the reaction of the uterus in preparation for and during implantation. De Feo (1967) reviewed this subject very thoroughly in the first edition of this book. The great majority of research investigations have been carried out on laboratory rodents, with very little on primates or the larger domestic animals. It follows therefore that any general account of implantation will lean heavily on information acquired from laboratory animals. Although one would like to have a much more general coverage, one cannot escape the reality that information acquired from many experiments on large numbers of laboratory animals provides a sound foundation on which to build. Information from primates or wild mammals will always tend to be fragmentary because of the difficulty of obtaining large numbers of specimens. Anyone who has worked with rats or mice knows how easy it is to be misled by the results from two or three animals. The only sensible course therefore is to get as

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Induction of Blastocyst Implantation in Mice by Cyclic Amp

Cited by 31 publications

References 7 publications

Potential Sites of Prostaglandin Actions in the Periimplantation Mouse Uterus: Differential Expression and Regulation of Prostaglandin Receptor Genes1

Potential Sites of Prostaglandin Actions in the Periimplantation Mouse Uterus: Differential Expression and Regulation of Prostaglandin Receptor Genes1

The placenta: transcriptional, epigenetic, and physiological integration during development

The Implantation Reaction

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