Induction of arthritis in monkeys by immunization with type II collagen.

Abstract

Immunization of two cynomolugus and three rhesus monkeys with purified type II collagen resulted in the development of polyarthritis. Arthritis first became clinically apparent 7 wk after primary immunization and persisted for 16 mo. Radiologic examination of the limbs demonstrated soft tissue swelling with severe joint destruction including loss of cartilage and bone. Involved joints eventually became ankylosed with permanent loss of some motion. All of the monkeys developed a response to the immunizing collagen as determined by ELISA of serum for antibodies. Arthritis was associated with weight loss and constitutional symptoms, including lethargy and refusal to eat. One monkey became so debilitated that it was necessary to euthanize it. Histologic examination of the joints showed synovial hypertrophy with pannus formation. A control monkey immunized with type I collagen suffered no apparent ill effects.

Cloricromene, a coumarine derivative, protects against collagen‐induced arthritis in Lewis rats

“…Collagen-induced arthritis (CIA) is an experimental model of autoimmune disease, which can be induced in mice (Yoo et al, 1988), rats (Cuzzocrea et al, 1999a,b) and primates (Trentham, 1982) by immunization with type II native articular cartilage collagen (CII). The joint pathology associated with CIA is similar to the one observed in patients with RA (Stuart et al, 1982a,b).…”

Crystallographic Structure of a Rheumatoid Arthritis MHC Susceptibility Allele, HLA-DR1 (DRB1*0101), Complexed with the Immunodominant Determinant of Human Type II Collagen

“…Both B cell (5,6) and T cell (7)(8)(9) immunity to CII have been reported, and the presence of CII Abs and CII-specific T cells in arthritic joints has been demonstrated (6,9,10). Additionally, a number of animal models have been described in which immunization with CII elicits an autoimmune arthritis that shares many similarities with RA (11)(12)(13)(14)(15)(16). In recent years, several mouse models have been developed in which the susceptibility to collagen-induced arthritis (CIA) is dependent on the transgenic expression of HLA-DR alleles associated with susceptibility to RA (12,13,17).…”

HLA-DQB1 polymorphism determines incidence, onset, and severity of collagen-induced arthritis in transgenic mice. Implications in human rheumatoid arthritis.

“…Studies in RA patients have shown antibody and T cell activity directed to intact and denatured CII (36)(37)(38). Furthermore, injection of heterologous CII into rats, mice, and monkeys results in joint inflammation and pathology with considerable similarity to human RA (35,39,40). Collagen-induced arthritis (CIA) is linked to MHC class II genes, similar to RA (41).…”