Induction of apoptosis in rat lung epithelial cells by multiwalled carbon nanotubes

Ravichandran, Prabakaran; Periyakaruppan, Adaikkappan; Sadanandan, Bindu; Ramesh, Vani; Hall, Joseph; Jejelowo, Olufisayo; Ramesh, Govindarajan T.

doi:10.1002/jbt.20296

Cited by 61 publications

(30 citation statements)

References 38 publications

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“…In vitro studies reported various toxicological effects of MW-CNTs including oxidative stress, DNA damage and apoptosis in mammalian cells lines. Other effects include VE-cadherin distribution and actin filament integrity in human aortic endothelial cells [114][115][116][117][118].…”

Section: Effects On Cell Linesmentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

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Section: Effects On Cell Linesmentioning

confidence: 99%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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“…Our earlier studies using different nanoparticles such as single and multi walled carbon nanotubes showed significantly increased levels of ROS at 5-10 µg/ml. [19][20][21][22], whereas, here in ZnO nanorods, we did not detect any increase in ROS level even in 20 µg/ml (data not shown). The time kinetics was also performed to check the formation of ROS (see Fig.…”

Section: Resultsmentioning

confidence: 49%

“…Also, 5 mM of ZnO nanoparticle are shown to be less toxic to human T cells [27]. Previous studies from our laboratory on hela cells and other cells such as lung epithelial, H1299, A549 and HaCaT cells showed the decrease in cell viability at 5 µg/ml when they were exposed to SWCNT and MWCNT [19][20][21][22].…”

Section: Resultsmentioning

confidence: 83%

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Synthesis, characterization and biocompatibility studies of zinc oxide (ZnO) nanorods for biomedical application

et al. 2010

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Nanoparticles are increasingly being recognized for their potential utility in biological applications including nanomedicine. Here, we have synthesized zinc oxide (ZnO) nanorods using zinc acetate and hexamethylenetetramine as precursors followed by characterizing using X-ray diffraction, fourier transform infrared spectroscopy, scanning electron microscopy and transmission electron microscopy. The growth of synthesized zinc oxide nanorods was found to be very close to its hexagonal nature, which is confirmed by X-ray diffraction. The nanorod was grown perpendicular to the long-axis and grew along the [001] direction, which is the nature of ZnO growth. The morphology of synthesized ZnO nanorods from the individual crystalline nucleus was confirmed by scanning and transmission electron microscopy. The length of the nanorod was estimated to be around 21 nm in diameter and 50 nm in length. Our toxicology studies showed that synthesized ZnO nanorods exposure on hela cells has no significant induction of oxidative stress or cell death even in higher concentration (10 μg/ml). The results suggest that ZnO nanorods might be a safer nanomaterial for biological applications.Keywords: Zinc oxide [ZnO]; Nanorods; XRD; SEM & TEM; Cytotoxicity Citation: R. Gopikrishnan, K. Zhang, P. Ravichandran, S. Baluchamy, V. Ramesh, S. Biradar, P. Ramesh, J. Pradhan, J. C. Hall, A. K. Pradhan and G. T. Ramesh, "Synthesis, characterization and biocompatibility studies of zinc oxide (ZnO) nanorods for biomedical application", Nano-Micro Lett. 2, 31-36 (2010). doi: 10.5101/nml.v2i1.p31-36 Nanotechnology has extraordinary potential to change our lives by improving existing products and enabling new ones. It facilitates the development of new materials in the 1a100 nm range, comparable to the size range of biological molecules and structures [1]. Nanomaterials are very attractive materials for the manipulation, sensing and detection of biological structures and systems [2]. The principal factors which make nanomaterials different from their bulk counterparts include an increase in their relative surface area and quantum effects, which affect their physical and chemical properties [2]. This is due to the large surface area-to-volume ratio of nanoparticles, which increases surface free energy to a point that is comparable to their lattice energy. For example, a particle of 30 nm size has 5% of its atoms on its surface compared to 50% of the atoms on the surface of a 3 nm particle [3]. The altered properties of nanomaterials, and their size similarity to naturally occurring cell structures, will allow them to interact readily with bio molecules and potentially affect the cellular responses in a dynamic and selective manner. Materials that exploit these characteristics are becoming increasingly attractive for use in novel biomedical applications.Nano structured materials exhibit unique properties related to their size, and are used in an array of applications such as optoelectronics, nano/microelectronics, sensors, transducers, cosmetics as w...

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“…104 Still, because of their fiber shape and size, carbon nanotubes cause cytotoxicity, inflammation, and DNA damage in vitro. [193][194][195][196] The animal models used to study the toxic effects of carbon nanotubes demonstrated that the high concentrations needed to induce the regression of the tumor may cause chronic lung inflammation, foreign-body granulomas, or interstitial fibrosis, [197][198][199][200] limiting their potential clinical use.…”

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confidence: 99%

Nanopharmacology in translational hematology and oncology

Tomuleasa¹,

Braicu²,

Irimie³

et al. 2014

IJN

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Nanoparticles have displayed considerable promise for safely delivering therapeutic agents with miscellaneous therapeutic properties. Current progress in nanotechnology has put forward, in the last few years, several therapeutic strategies that could be integrated into clinical use by using constructs for molecular diagnosis, disease detection, cytostatic drug delivery, and nanoscale immunotherapy. In the hope of bringing the concept of nanopharmacology toward a viable and feasible clinical reality in a cancer center, the present report attempts to present the grounds for the use of cell-free nanoscale structures for molecular therapy in experimental hematology and oncology.

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Induction of apoptosis in rat lung epithelial cells by multiwalled carbon nanotubes

Cited by 61 publications

References 38 publications

In vitro and in vivo toxicity assessment of nanoparticles

In vitro and in vivo toxicity assessment of nanoparticles

Synthesis, characterization and biocompatibility studies of zinc oxide (ZnO) nanorods for biomedical application

Nanopharmacology in translational hematology and oncology

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