Induction of Apoptosis in HT-29 Human Colon Cancer Cells by the Pepper Component Piperine

김은지,; 박희숙,; 신민정,; 신현경,; 윤정한,

doi:10.3746/jkfn.2009.38.4.442

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…In a comparison with the control group, DAPI-positive cells increased by more than 3-fold depending on the concentration of piperine used. Shin et al reported the concentration-dependent increase in apoptotic bodies in AGS stomach cancer cells treated with piperine in (100 and 150 μM) (27), while Kim et al observed apoptotic bodies in HT-29 colon cancer cells treated with 40 μM piperine (28). These findings suggest that the decreases in the survival rates of A375SM and A375P treated with piperine were caused by apoptosis induction.…”

Section: Discussionmentioning

confidence: 98%

Antitumor and Apoptosis-inducing Effects of Piperine on Human Melanoma Cells

et al. 2019

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Background/Aim: Piperine is a major pungent alkaloid present in black pepper (Piper nigrum L). This study investigated the potential anticancer effects of piperine on human melanoma cells and explored the potential pharmacological mechanisms in vitro and in vivo. Materials and Methods: Studies were performed using the MTT assay,4', staining, western blotting, a xenograft model, the terminal deoxynucleotidyl transferase dUTP nick end labeling assay, and immunohistochemistry. Results: Piperine inhibited the growth of melanoma cells. Several apoptotic events were observed following treatment, as revealed by DAPI staining. Piperine increased the expression of BCL2-associated X, apoptosis regulator (BAX), cleaved poly(ADP-ribose)polymerase, cleaved caspase-9, phospho-c-Jun N-terminal kinase and phospho-p38, and reduced that of B-cell lymphoma 2 (BCL2), X-chromosome-linked inhibitor of apoptosis, and phosphoextracellular signal-regulated protein kinase (ERK1/2) in a concentration-dependent manner. Treatment of mice for 4 weeks with piperine inhibited tumor growth without apparent toxicity. Piperine increased the expression of apoptotic cells and cleaved-caspase-3 protein and reduced the expression of phospho-ERK1/2 protein in melanoma tumors. Conclusion: Piperine suppressed the growth of human melanoma cells by the induction of apoptosis via the inhibition of tumor growth of human melanoma cells and tumor xenograft models.The mortality rate of cancer is increasing every year worldwide. Cancer is also the leading cause of death in South Korea (1, 2). The occurrence of skin cancer has consistently increased with concurrent increases in ultraviolet light exposure during outdoor activities. Comprising only 4% of all types of skin cancers, but with a mortality as high as 80%, melanoma has the highest rate of skin cancer malignancies and is caused by the malignant alteration of melanin cells (3,4). Malignant human melanoma cells rapidly spread to internal organs such as the bones, liver and lungs through lymphatic ducts and blood vessels. Due to its high resistance to chemotherapy and radiotherapy, surgical excision after early detection is considered the only treatment for this disease. Human melanoma is an intractable disease, and no specific treatment is available in cases of recurrence (5,6). A specific group of food materials, medications, and health supplements developed from various natural resources have also been used to treat melanoma (7).Piper nigrum L., a member of the family Piperaceae, is used as spice in Asia and India, and has beneficial effects on dyspepsia and pain relief due to the presence of various phytochemicals with different biological activities (8). Piperine (Figure 1) is a major alkaloid-amide that is responsible for the distinct taste and scent of pepper (9). Piperine also reportedly has various biological activities including antioxidant (10), anti-inflammatory (11), antiarthritic (12), antibacterial (13), and anticancer (14).

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Section: Discussionmentioning

confidence: 98%

Antitumor and Apoptosis-inducing Effects of Piperine on Human Melanoma Cells

et al. 2019

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“…Piperine inhibits IL-1 β-induced p38 MAPK and STAT3 activation and, in turn, blocks the IL-1 β-induced IL-6 expression in TMK-1 gastric cancer cells [67]. It also decreases the protein levels of Bcl-2, Mcl-1, survivin, and increases the Fas levels, resulting in inhibition of the growth of HT-29, human colon cancer cells [68]. In HT-29 colon carcinoma cells, piperine reduces the levels of cyclins (D1 and D3), cyclin-dependent kinases (CDK-4 and 6), and upregulates p21/WAF1 and p27/KIP1 expression [69].…”

Section: Cancers Of the Gastrointestinal Tractmentioning

confidence: 99%

Piperine-A Major Principle of Black Pepper: A Review of Its Bioactivity and Studies

et al. 2019

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Piperine is the main compound present in black pepper, and is the carrier of its specific pungent taste, which is responsible for centuries of human dietary utilization and worldwide popularity as a food ingredient. Along with the application as a food ingredient and food preservative, it is used in traditional medicine for many purposes, which has in most cases been justified by modern scientific studies on its biological effects. It has been confirmed that piperine has many bioactive effects, such as antimicrobial action, as well as many physiological effects that can contribute to general human health, including immunomodulatory, hepatoprotective, antioxidant, antimetastatic, antitumor, and many other activities. Clinical studies demonstrated remarkable antioxidant, antitumor, and drug availability-enhancing characteristics of this compound, together with immunomodulatory potential. All these facts point to the therapeutic potential of piperine and the need to incorporate this compound into general health-enhancing medical formulations, as well as into those that would be used as adjunctive therapy in order to enhance the bioavailability of various (chemo)therapeutic drugs.

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“…Inside the pepper containing piperine. As reported by Kim et al (2010) and Yaffe et al (2015), Piperine is effective to inhibit the growth of colon cancer cells in laboratory work [9,10]. To know how potential piperine able to be an alternative compound for colon cancer treatment, besides a laboratory experiment that had been conducted by Kim et al and Yaffe et al, the affinity of piperine toward responsible protein targeting for colon cancer is also necessary to be evaluated.…”

Section: Resultsmentioning

confidence: 99%

“…The biological activity of piperine has been reported by many researchers, such as increases in the absorption of nutrients in the body, anti-inflammatory, analgesic, anticarcinogenic, antimutagenic, and antitumor [6][7][8]. Piperine also reported inhibiting the growth of colon cancer cells in laboratory work [9,10]. Ranged 35-77% of cases of colon cancer were found to be an excessive expression of epidermal growth factor receptor (EGFR) because of carcinogenic stimulation.…”

Section: Introductionmentioning

confidence: 99%

Molecular Docking for Evaluation of Piperine Affinity to the Colon Cancer Receptor

Harimurti

Widada

Arsito

et al. 2022

Kme

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Piperine is an alkaloid found in the plants of the Piperaceae family, such as Piper nigrum and Piper retrofractum. These two plants are commonly used as flavoring agents in daily meals. The piperine extract is reported to be effective in inhibiting the growth of colon cancer cells in laboratory experiments. Between 35-77% of colon cancer cases are caused by excessive expression of the epidermal growth factor receptor. In developing a new compound for commercial drugs, a study of the compound affinity to the receptor is necessary. Hence, the purpose of this study was to comprehensively evaluate the affinity of piperine to the colon cancer receptor using molecular docking software. The AutoDock software was used as a tool for the analysis. Three compounds were analyzed, i.e., the tested ligand (piperine), a native ligand (N-acetyl-D-glucosamine), and a positive control ligand (Gefitinib). Further, the binding score of piperine to the receptor was compared to the binding score of native ligands and positive control ligands. The binding score of the three compounds was found to be -3.82 kcal/mole (piperine), -4.16 kcal/mole (Gefitinib), and -3.75 kcal/mole (N-acetyl-D-glucosamine). It can therefore be concluded that there is a similar affinity between piperine and the control ligand. Therefore, piperine can be recommended and developed as a new drug for colon cancer treatment. Keywords: piperine, in silico, colon cancer, molecular docking

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Induction of Apoptosis in HT-29 Human Colon Cancer Cells by the Pepper Component Piperine

Cited by 6 publications

References 28 publications

Antitumor and Apoptosis-inducing Effects of Piperine on Human Melanoma Cells

Antitumor and Apoptosis-inducing Effects of Piperine on Human Melanoma Cells

Piperine-A Major Principle of Black Pepper: A Review of Its Bioactivity and Studies

Molecular Docking for Evaluation of Piperine Affinity to the Colon Cancer Receptor

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