Induction of apoptosis by l-carnitine through regulation of two main pathways in Hepa1c1c 7 cells

Fan, Jiang Ping; Kim, Han Seop; Han, Gi Dong

doi:10.1007/s00726-008-0093-y

Cited by 21 publications

(17 citation statements)

References 27 publications

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“…Cell viability assay was carried out using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich Inc., St. Louis, MO) assay as described previously (Fan et al 2009). Briefly, cells were plated on 96-well plates at an initial density of 1 Â 10 5 cells per well, followed by the addition of LF extract (aliquots were 50, 100, 200, and 500 mg/mL of lyophilised powder dissolved in medium and filter-sterilised) with or without 100 mM EtOH for 24 h. Cell viability was determined based on the formazan production by MTT assay using DMSO:EtOH (1:1 v/v) solution at an absorbance of 540 nm using a microplate reader (TECAN, Vienna, Austria).…”

Section: Cell Viability Assaymentioning

confidence: 99%

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Ligularia fischeriextract attenuates liver damage induced by chronic alcohol intake

Kim

Lee

et al. 2016

Pharmaceutical Biology

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Context Ligularia fischeri (Ledebour) Turcz. (Compositae) has been used as a leafy vegetable and in traditional medicine to treat hepatic disorder in East Asia. Objective The present study explores the antioxidant activity of LF aqueous extract on EtOHinduced oxidative stress accompanied by hepatotoxicity both in vitro and in vivo. Materials and methods In vitro study using the mouse liver NCTC-1469 cell line was conducted to estimate the cytotoxicity as well as the inhibitory effect of LF extract against alcohol-treated cell damage. In vivo study used an alcohol-fed Wister rat model orally administered EtOH (3.95 g/kg of body weight/d) with or without LF extract (100 or 200 mg/kg body weight) for 6 weeks. Serum and liver tissue were collected to evaluate hepatic injury and antioxidant-related enzyme activity. Results The EC 50 value for the DPPH radical scavenging capacity of LF extract was 451.5 mg/mL, whereas the IC 50 value of LF extract in terms of EtOH-induced reactive oxygen species (ROS) generation was 98.3 mg/mL without cell cytotoxicity. LF extract (200 mg/kg body weight) significantly reduced the triglyceride content of serum (33%) as well as hepatic lipid peroxidation (36%), whereas SOD activity was elevated three-fold. LF extract suppressed expression of CYP2E1 and TNF-a, and attenuated alcohol-induced abnormal morphological changes. Discussion and conclusion LF extract attenuated liver damage induced by alcoholic oxidative stress through inhibition of ROS generation, down-regulation of CYP2E1, and activation of hepatic antioxidative enzymes. Homeostasis of the antioxidative defence system in the liver by LF extract mitigated hepatic disorder following chronic alcohol intake. ARTICLE HISTORY

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Section: Cell Viability Assaymentioning

confidence: 99%

“…Semi-quantitative RT-PCR of total RNA in the liver was performed according to a previously described method (Fan et al 2009). Five micrograms of total RNA was used for first-strand cDNA synthesis.…”

Section: Reverse Transcriptase-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Ligularia fischeriextract attenuates liver damage induced by chronic alcohol intake

Kim

Lee

et al. 2016

Pharmaceutical Biology

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“…The samples were then dissolved in distilled water and treated at a final concentration of 1 mg/mL. Cell viability Cell viability was examined using the MTT assay (14). 3T3-L1 preadipocytes were placed into each well of a 96-well plate, followed by incubation at 37 o C in a 5% CO 2 incubator for 24 h. SEs diluted with DMEM to concentrations of 0-1,000 μg/mL were added to the wells, and the plate was incubated for an additional 24 h. MTT (final concentration of 0.25 μg/mL in phosphate-buffered saline (PBS)) was added for 4 h, after which formazan crystals were dissolved in dimethyl sulfoxide and absorbance was measured at 540 nm using a microplate reader (Sunrise, Tecan, Vienna, Austria).…”

Section: Methodsmentioning

confidence: 99%

“…RT-PCR of total RNA from 3T3-L1 adipocytes was performed according to a previously described method (14). Total RNA (5 μg) was used for first-strand cDNA synthesis.…”

Section: Rna Isolation and Semi-quantitative Reverse Transcription (Rmentioning

confidence: 99%

Sigumjang (fermented barley bran) water-soluble extracts inhibit the expression of adipogenic and lipogenic regulators in 3T3-L1 adipocytes

Jeong

Lee

Kim

et al. 2016

Food Sci Biotechnol

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prepared from fermented barley bran is a traditional fermented food found only in the Gyeongsang-do area of South Korea. There have been no studies reported to date despite the potential bioactivities of . In this study, the anti-obesity activities of extracts (SEs) during 3T3-L1 differentiation into adipocytes were investigated. SEs inhibited adipocyte differentiation by suppressing the CCAAT/enhancer binding protein-β and sterol regulatory element binding protein-1c expression in the early stage of differentiation, followed by the suppression of the peroxisome proliferator-activated receptor-γ, CCAAT/enhancer binding protein-α, and adiponectin. These changes in adipogenic markers induced inhibition of lipogenesis via down-regulation of mainly fatty acid synthase, acetyl-CoA carboxylase, fatty acid binding protein 4, and perilipin. These results were more significant in the extract of fermented with isolated MFST compared to naturally fermented group. SEs can be considered as a useful material for developing food with health benefits and anti-obesity properties.

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“…13 Preadipocytes (1 × 10 4 ) were placed into each well of a 96-well plate, after which they were incubated at 37°C in a 5% CO 2 incubator for 24 hours. MTT (final concentration 0.4 µg/mL in phosphate-buffered saline) was added after 24 hours.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Bioavailability of nanoemulsified conjugated linoleic acid for an antiobesity effect

Kim¹,

Park²,

Kweon³

et al. 2013

IJN

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Background:The aim of this study was to enhance the bioavailability of conjugated linoleic acid (CLA), which has low water solubility, using nanoemulsion technology and to evaluate the effects of its improved bioavailability as an antiobesity agent. Methods: The antiobesity effect of nanoemulsified water-soluble conjugated linoleic acid (N-CLA) was evaluated using in vitro and in vivo studies. Differentiated 3T3-L1 adipocytes were treated with CLA and N-CLA to assess their lipolytic effect. Further, to confirm the antiobesity effect of N-CLA, male Sprague-Dawley rats were randomly separated into four groups, ie, a group fed a normal diet, a group fed a high-fat diet (obesity rat model), a CLA-treated group, and an N-CLA-treated group. Results: N-CLA showed a greater lipolytic effect on differentiated 3T3-L1 adipocytes compared with normal CLA. N-CLA enhanced the release of glycerol from triglycerides, which accumulated in differentiated 3T3-L1 adipocytes. Further, N-CLA enhanced leptin secretion to an extent similar to that of orlistat, an antiobesity agent. In an animal obesity model fed a high-fat diet, N-CLA attenuated accumulation of triglycerides, total cholesterol, and low-density lipoprotein cholesterol in serum, and also significantly decreased the volume of triglycerides and cholesterol in liver tissue. Conclusion: These results indicate that N-CLA has a greater antiobesity effect than CLA as a result of its improved bioavailability.

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Induction of apoptosis by l-carnitine through regulation of two main pathways in Hepa1c1c 7 cells

Cited by 21 publications

References 27 publications

Ligularia fischeriextract attenuates liver damage induced by chronic alcohol intake

Ligularia fischeriextract attenuates liver damage induced by chronic alcohol intake

Sigumjang (fermented barley bran) water-soluble extracts inhibit the expression of adipogenic and lipogenic regulators in 3T3-L1 adipocytes

Bioavailability of nanoemulsified conjugated linoleic acid for an antiobesity effect

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