Induction of apoptosis by hydroalcoholic and methanolic extract of Deracocephalum kotschyi on Trichomonas vaginalis

Dehkordi, Bahareh Chelgerdi; Karimi-Dehkordi, Maryam; Khamesipour, Faham

doi:10.1007/s12639-022-01482-6

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…Aromatic plants have long been used to manage several ailments and for various pharmacological uses. Their medical importance is mainly due to their essential oil fractions (Chelgerdi Dehkordi et al., 2022 ; Rajesh & Howard, 2003 ). Essential oils are natural products abundant in nature and have been considered a potential source of antibacterial and antifungal molecules presenting few side effects.…”

Section: Introductionmentioning

confidence: 99%

Induction of apoptosis by essential oil of Dracocephalum kotschyi on Trichomonas vaginalis

Dehkordi

Esmaeilifallah

Kalantari

et al. 2022

Veterinary Medicine & Sci

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Background Trichomonas vaginalis is a protist parasite that causes trichomoniasis, a sexually transmitted disease. Metronidazole is the current treatment for trichomoniasis. However, this drug can provoke severe side effects, and some strains present resistance, making the development of alternative treatments for trichomoniasis urgent. Objectives We investigate the use of essential oil obtained from Dracocephalum kotschyi on T. vaginalis. D. kotschyi has antispasmodic and analgesic properties and is well known in Iran. Methods The essential oil was obtained by hydrodistillation from 1000 g of the powdered plant. Gas chromatography‐mass spectrometry analysis was used for the chemical composition of the essential oil, and 11 substances were identified, corresponding to 91.5% of the oil. Copaene (22.15%), Methyl geranate (16.31%), Geranial (13.78%) and Carvone (11.34%) were the main substances. A cell viability test was used to determine the percentage of growth inhibition (GI%) and the half‐maximal inhibitory concentration (IC50) on T. vaginalis after incubation with the prepared essential oil. Results The oil induced an IC50 of 84.07 µg/ml after 24 h contact with trophozoites. Cytotoxicity was determined by MTT assay on the J774.A1 haematopoietic cell line. In addition, the initial stage of apoptosis was assayed using the fluorescein isothiocyanate Annexin V Apoptosis Detection Kit. Evaluation of the in vitro anti‐trichomonal properties of D. kotschyi essential oils showed that it effectively induces apoptosis on T. vaginalis between 100 and 700 µg/ml after 48 h without toxicity on haematopoietic cells, suggesting that D. kotschyi essential oil can induce programmed death in T. vaginalis. Conclusions The anti‐trichomonal properties of D. kotschyi essential oil indicate that they could be suitable for new pharmacologic studies after new tests with human vaginal epithelial cells.

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Section: Introductionmentioning

confidence: 99%

Induction of apoptosis by essential oil of Dracocephalum kotschyi on Trichomonas vaginalis

Dehkordi

Esmaeilifallah

Kalantari

et al. 2022

Veterinary Medicine & Sci

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Dracocephalum kotschyi Boiss. In Vitro Efficacy on Growth and Apoptosis Induction in Leishmania major Promastigotes

Kosari

Khamesipour

2022

BioMed Research International

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Dracocephalum kotschyi Boiss. is a plant generally used in modern medicine to treat many human illnesses. It is also used to prevent tumor cell proliferation throughout the world. This study’s objective was to evaluate this plant’s in vitro efficacy on growth and apoptosis induction in Leishmania major promastigotes. To do this, the essential oil is extracted for the test following the collection and identification of D. kotschyi. The essential oil was analyzed using a GC-MS analyzer. Promastigotes of L. major were cultured in RPMI-1640 media, and the MTT assay and a flow cytometry analysis were carried out on promastigotes that had entered the log phase. To differentiate between viable, necrotic, and apoptotic treated or untreated promastigotes, the flow cytometry method of double staining with annexin V-FLUOS and propidium iodide (PI) was used. Given the results obtained, 11 phytochemicals were identified in the essential oil of this plant. Copaene (22.15%), methyl geranate (16.31%), geranial (13.78%), and carvone (11.34%) were the main substances. The essential oil of D. kotschyi inhibits the proliferation of L. major promastigotes at 921 μg/mL, 252 μg/mL, and 416 μg/mL, respectively, after 24 h, 48 h, and 78 h. The cells were divided into four quadrates based on cell phases using the flow cytometry approach by double staining with annexin V-FLUOS and propidium iodide (PI): necrosis (Q1), late apoptosis (Q2), early apoptosis (Q3), and viable (Q4) quadrates. Overall, it is apparent that the different concentrations induced cell apoptosis in promastigotes. Observation under the light microscope at ×100 magnification showed that the different doses of D. kotschyi essential oil caused apparent alterations in the treated promastigotes. In this work, D. kotschyi essential oils induce programmed death on L. major promastigotes. This study opens many research perspectives, such as investigating the mechanisms of action and the production of a phytomedicine based on this plant.

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Induction of apoptosis by hydroalcoholic and methanolic extract of Deracocephalum kotschyi on Trichomonas vaginalis

Cited by 2 publications

References 26 publications

Induction of apoptosis by essential oil of Dracocephalum kotschyi on Trichomonas vaginalis

Induction of apoptosis by essential oil of Dracocephalum kotschyi on Trichomonas vaginalis

Dracocephalum kotschyi Boiss. In Vitro Efficacy on Growth and Apoptosis Induction in Leishmania major Promastigotes

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