Induction of Apoptosis by Hybrid Liposomes without Antitumor Drugs toward Human Breast Tumor Cells

Nagami, Hideaki; Yamamoto, Keiichi; Ichihara, Hideaki; Matsumoto, Yōko; Ueoka, Ryuichi

doi:10.1252/jcej.36.1127

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…In our study, we clearly demonstrated that hybrid liposomes composed of 90 mol% DMPC and 10 mol% C 12 (EO) 10 24 and difference in the effect of hybrid liposomes on nontumor (normal line) cells vs. tumor (hepatoma) cells has been recently obtained. 25 We are now establishing a novel approach to cancer chemotherapy using hybrid liposomes composed of 90 mol% DMPC and 10 mol% C 12 (EO) 10 .…”

Section: Resultssupporting

confidence: 52%

“…The present study also explains the novel mechanism of apoptosis induced by hybrid liposomes without antitumor agents. Inhibitory effects of hybrid liposomes on several tumor cell lines [brain (U251), stomach (GT3-TKB) and breast tumor cells (MDA-MB-453)] 24 and difference in the effect of hybrid liposomes on nontumor (normal line) cells vs. tumor (hepatoma) cells has been recently obtained. 25 We are now establishing a novel approach to cancer chemotherapy using hybrid liposomes composed of 90 mol% DMPC and 10 mol% C 12 (EO) 10 .…”

Section: Resultsmentioning

confidence: 99%

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Novel mechanism of hybrid liposomes‐induced apoptosis in human tumor cells

Matsumoto

Iwamoto

Matsushita

et al. 2005

Intl Journal of Cancer

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Hybrid liposomes can be prepared by simply ultrasonicating a mixture of vesicular and micellar molecules in a buffer solution. The physical properties of these liposomes, such as size, membrane fluidity, phase transition temperature and hydrophobicity can be controlled by changing the composition. Hybrid liposomes composed of dimyristoylphosphatidylcholine and polyoxyethylene (10) dodecyl ether were found to inhibit the growth of human promyelocytic leukemia (HL-60) cells without using any drugs. Induction of apoptosis by hybrid liposomes in HL-60 cells was verified on the basis of fluorescence microscopy and flow cytometry analysis, after fusion and accumulation of hybrid liposomes, which was revealed on the basis of microphysiometer. We elucidated the pathways of apoptosis induced by the hybrid liposomes. That is, hybrid liposomes fused and accumulated in tumor cell membranes, and the apoptosis signal first passed through mitochondria, caspase-9 and caspase-3, second through Fas, caspase-8, caspase-3 and then reached the nucleus. Hybrid liposomes themselves can induce apoptosis in human tumor cells along with high inhibitory effects on the growth of tumor cells. ' 2005 Wiley-Liss, Inc.

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